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Most microorganisms have either the closed tricarboxylic acid cycle or the complete Wood-Ljungdahl-pathway for acetyl-CoA oxidation, but the sulfate-reducing, naphthalene-degrading culture N47 possesses both completely. Combining <jats:sup>13<\/jats:sup>C- labeled substrates and mass-specific GC-MS analysis of amino acids and fatty acids with enzyme activity assays suggests that N47 has a chemoorganoautotrophic metabolism degrading complex organic substrates such as naphthalene. Surprisingly, however, the biomass is mainly produced from acetyl-CoA generated de novo via CO<jats:sub>2<\/jats:sub>-fixation. This metabolism probably requires both a complete Wood-Ljungdahl pathway for acetyl-CoA oxidation and a reverse tricarboxylic acid cycle for CO<jats:sub>2<\/jats:sub> fixation. 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