{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:54:50Z","timestamp":1782878090697,"version":"3.54.5"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100000057","name":"U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35-GM126974"],"award-info":[{"award-number":["R35-GM126974"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32-AR007612"],"award-info":[{"award-number":["T32-AR007612"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01-HL158795"],"award-info":[{"award-number":["R01-HL158795"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01-HL162927"],"award-info":[{"award-number":["R01-HL162927"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["PRE1019410"],"award-info":[{"award-number":["PRE1019410"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"DOI":"10.1038\/s42003-025-08362-8","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T18:51:21Z","timestamp":1750272681000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Estradiol pauses microtubule growth without increased incidence of catastrophe events"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-3968","authenticated-orcid":false,"given":"Allison N.","family":"Ogren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7460-7429","authenticated-orcid":false,"given":"Noah J.","family":"Zimmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Stark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6636-5238","authenticated-orcid":false,"given":"Mark","family":"McClellan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel J.","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7926-3342","authenticated-orcid":false,"given":"Lynn M.","family":"Hartweck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0364-6742","authenticated-orcid":false,"given":"Kurt W.","family":"Prins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-7363","authenticated-orcid":false,"given":"Melissa K.","family":"Gardner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"8362_CR1","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1161\/CIRCULATIONAHA.119.043930","volume":"141","author":"MA Caporizzo","year":"2020","unstructured":"Caporizzo, M. A., Chen, C. Y., Bedi, K., Margulies, K. B. & Prosser, B. L. Microtubules increase diastolic stiffness in failing human cardiomyocytes and myocardium. Circulation 141, 902\u2013915 (2020).","journal-title":"Circulation"},{"key":"8362_CR2","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1177\/1535370219868960","volume":"244","author":"MA Caporizzo","year":"2019","unstructured":"Caporizzo, M. A., Chen, C. Y. & Prosser, B. L. Cardiac microtubules in health and heart disease. Exp. Biol. Med. (Maywood) 244, 1255\u20131272 (2019).","journal-title":"Exp. Biol. Med. (Maywood)"},{"key":"8362_CR3","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1038\/s41569-022-00692-y","volume":"19","author":"MA Caporizzo","year":"2022","unstructured":"Caporizzo, M. A. & Prosser, B. L. The microtubule cytoskeleton in cardiac mechanics and heart failure. Nat. Rev. Cardiol. 19, 364\u2013378 (2022).","journal-title":"Nat. Rev. Cardiol."},{"key":"8362_CR4","doi-asserted-by":"publisher","first-page":"2252","DOI":"10.1016\/j.devcel.2021.07.004","volume":"56","author":"RR Goldblum","year":"2021","unstructured":"Goldblum, R. R. et al. Oxidative stress pathogenically remodels the cardiac myocyte cytoskeleton via structural alterations to the microtubule lattice. Dev. Cell 56, 2252\u20132266.e2256 (2021).","journal-title":"Dev. Cell"},{"key":"8362_CR5","doi-asserted-by":"crossref","unstructured":"Prins, K. W. et al. Colchicine depolymerizes microtubules, increases junctophilin-2, and improves right ventricular function in experimental pulmonary arterial hypertension. J. Am. Heart Assoc. 6, e006195 (2017).","DOI":"10.1161\/JAHA.117.006195"},{"key":"8362_CR6","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.1093\/carcin\/15.9.1875","volume":"15","author":"E Aizu-Yokota","year":"1994","unstructured":"Aizu-Yokota, E., Ichinoseki, K. & Sato, Y. Microtubule disruption induced by estradiol in estrogen receptor-positive and -negative human breast cancer cell lines. Carcinogenesis 15, 1875\u20131879 (1994).","journal-title":"Carcinogenesis"},{"key":"8362_CR7","first-page":"1863","volume":"55","author":"E Aizu-Yokota","year":"1995","unstructured":"Aizu-Yokota, E., Susaki, A. & Sato, Y. Natural estrogens induce modulation of microtubules in Chinese hamster V79 cells in culture. Cancer Res. 55, 1863 (1995).","journal-title":"Cancer Res."},{"key":"8362_CR8","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1159\/000070281","volume":"77","author":"JL Kipp","year":"2003","unstructured":"Kipp, J. L. & Ramirez, V. D. Estradiol and testosterone have opposite effects on microtubule polymerization. Neuroendocrinology 77, 258\u2013272 (2003).","journal-title":"Neuroendocrinology"},{"key":"8362_CR9","doi-asserted-by":"publisher","first-page":"659","DOI":"10.2174\/1381612824666180123110042","volume":"24","author":"C Angelidis","year":"2018","unstructured":"Angelidis, C. et al. Colchicine pharmacokinetics and mechanism of action. Curr. Pharm. Des. 24, 659\u2013663 (2018).","journal-title":"Curr. Pharm. Des."},{"key":"8362_CR10","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1002\/med.20097","volume":"28","author":"B Bhattacharyya","year":"2008","unstructured":"Bhattacharyya, B., Panda, D., Gupta, S. & Banerjee, M. Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin. Med. Res. Rev. 28, 155\u2013183 (2008).","journal-title":"Med. Res. Rev."},{"key":"8362_CR11","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1021\/ci9900682","volume":"40","author":"M Feher","year":"2000","unstructured":"Feher, M. & Schmidt, J. M. Multiple flexible alignment with SEAL:\u2009 a study of molecules acting on the colchicine binding site. J. Chem. Inf. Comput. Sci. 40, 495\u2013502 (2000).","journal-title":"J. Chem. Inf. Comput. Sci."},{"key":"8362_CR12","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/0163-7258(91)90067-V","volume":"51","author":"SB Hastie","year":"1991","unstructured":"Hastie, S. B. Interactions of colchicine with tubulin. Pharm. Ther. 51, 377\u2013401 (1991).","journal-title":"Pharm. Ther."},{"key":"8362_CR13","doi-asserted-by":"publisher","unstructured":"Hemmat, M., Braman, M., Escalante, D. & Odde, D. J. Poisson poisoning as the mechanism of action of the microtubule-targeting agent colchicine. Preprint at bioRxiv https:\/\/doi.org\/10.1101\/2020.03.25.007757 (2020).","DOI":"10.1101\/2020.03.25.007757"},{"key":"8362_CR14","doi-asserted-by":"publisher","first-page":"j5492","DOI":"10.1136\/bmj.j5492","volume":"360","author":"T Thenappan","year":"2018","unstructured":"Thenappan, T., Ormiston, M. L., Ryan, J. J. & Archer, S. L. Pulmonary arterial hypertension: pathogenesis and clinical management. BMJ 360, j5492 (2018).","journal-title":"BMJ"},{"key":"8362_CR15","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1016\/j.cub.2013.12.042","volume":"24","author":"SP Maurer","year":"2014","unstructured":"Maurer, S. P. et al. EB1 accelerates two conformational transitions important for microtubule maturation and dynamics. Curr. Biol. 24, 372\u2013384 (2014).","journal-title":"Curr. Biol."},{"key":"8362_CR16","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1091\/mbc.12.4.971","volume":"12","author":"NM Rusan","year":"2001","unstructured":"Rusan, N. M., Fagerstrom, C. J., Yvon, A. M. & Wadsworth, P. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin. Mol. Biol. Cell 12, 971\u2013980 (2001).","journal-title":"Mol. Biol. Cell"},{"key":"8362_CR17","doi-asserted-by":"publisher","first-page":"e00616","DOI":"10.1002\/prp2.616","volume":"8","author":"E Forkosh","year":"2020","unstructured":"Forkosh, E., Kenig, A. & Ilan, Y. Introducing variability in targeting the microtubules: review of current mechanisms and future directions in colchicine therapy. Pharm. Res. Perspect. 8, e00616 (2020).","journal-title":"Pharm. Res. Perspect."},{"key":"8362_CR18","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.ejmech.2017.12.029","volume":"144","author":"AA Ghawanmeh","year":"2018","unstructured":"Ghawanmeh, A. A. et al. Colchicine prodrugs and codrugs: chemistry and bioactivities. Eur. J. Med. Chem. 144, 229\u2013242 (2018).","journal-title":"Eur. J. Med. Chem."},{"key":"8362_CR19","doi-asserted-by":"publisher","first-page":"3230","DOI":"10.1002\/anie.200300615","volume":"43","author":"T Graening","year":"2004","unstructured":"Graening, T. & Schmalz, H. G. Total syntheses of colchicine in comparison: a journey through 50 years of synthetic organic chemistry. Angew. Chem. Int. Ed. Engl. 43, 3230\u20133256 (2004).","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"8362_CR20","doi-asserted-by":"publisher","first-page":"4121","DOI":"10.1021\/acs.jcim.4c00438","volume":"64","author":"S Kumari","year":"2024","unstructured":"Kumari, S. & Sobhia, M. E. Targeting an old foe for cancer: a molecular dynamics perspective to unravel the specific binding nature of 2-methoxy estradiol to human \u03b2-tubulin isotypes. J. Chem. Inf. Model. 64, 4121\u20134133 (2024).","journal-title":"J. Chem. Inf. Model."},{"key":"8362_CR21","doi-asserted-by":"publisher","first-page":"9406","DOI":"10.1158\/0008-5472.CAN-05-0088","volume":"65","author":"Y G\u00f6kmen-Polar","year":"2005","unstructured":"G\u00f6kmen-Polar, Y. et al. \u03b2-tubulin mutations are associated with resistance to 2-methoxyestradiol in MDA-MB-435 cancer cells. Cancer Res. 65, 9406\u20139414 (2005).","journal-title":"Cancer Res."},{"key":"8362_CR22","doi-asserted-by":"publisher","first-page":"3150","DOI":"10.1158\/1535-7163.MCT-08-0442","volume":"7","author":"TYE Liaw","year":"2008","unstructured":"Liaw, T. Y. E. et al. Class I \u03b2-tubulin mutations in 2-methoxyestradiol-resistant acute lymphoblastic leukemia cells: implications for drug-target interactions. Mol. Cancer Ther. 7, 3150\u20133159 (2008).","journal-title":"Mol. Cancer Ther."},{"key":"8362_CR23","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1021\/bi00118a015","volume":"31","author":"DA Skoufias","year":"1992","unstructured":"Skoufias, D. A. & Wilson, L. Mechanism of inhibition of microtubule polymerization by colchicine: inhibitory potencies of unliganded colchicine and tubulin\u2013colchicine complexes. Biochemistry 31, 738\u2013746 (1992).","journal-title":"Biochemistry"},{"key":"8362_CR24","doi-asserted-by":"publisher","first-page":"1342","DOI":"10.1016\/j.cub.2013.05.059","volume":"14","author":"CE Coombes","year":"2013","unstructured":"Coombes, C. E., Yamamoto, A., Kenzie, M. R., Odde, D. J. & Gardner, M. K. Evolving tip structures can explain age-dependent microtubule catastrophe. Curr. Biol. 14, 1342\u20131348 (2013).","journal-title":"Curr. Biol."},{"key":"8362_CR25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2108046119","volume":"119","author":"A Ogren","year":"2022","unstructured":"Ogren, A. et al. Kinesin-14 motors participate in a force balance at microtubule plus-ends to regulate dynamic instability. Proc. Natl Acad. Sci. USA 119, e2108046119 (2022).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"8362_CR26","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1016\/j.cub.2007.07.011","volume":"17","author":"HT Schek","year":"2007","unstructured":"Schek, H. T. et al. Microtubule assembly dynamics at the nanoscale. Curr. Biol. 17, 1445\u20131455 (2007).","journal-title":"Curr. Biol."},{"key":"8362_CR27","doi-asserted-by":"publisher","first-page":"2911","DOI":"10.1529\/biophysj.105.060913","volume":"89","author":"V VanBuren","year":"2005","unstructured":"VanBuren, V., Cassimeris, L. & Odde, D. J. Mechanochemical model of microtubule structure and self-assembly kinetics. Biophys. J. 89, 2911\u20132926 (2005).","journal-title":"Biophys. J."},{"key":"8362_CR28","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1007\/s12195-010-0155-6","volume":"4","author":"AO Demchouk","year":"2011","unstructured":"Demchouk, A. O., Gardner, M. K. & Odde, D. J. Microtubule tip tracking and tip structures at the nanometer scale using digital fluorescence microscopy. Cell. Mol. Bioeng. 4, 192\u2013204 (2011).","journal-title":"Cell. Mol. Bioeng."},{"key":"8362_CR29","doi-asserted-by":"publisher","first-page":"4314","DOI":"10.3390\/ijms21124314","volume":"21","author":"L Aryan","year":"2020","unstructured":"Aryan, L. et al. The role of estrogen receptors in cardiovascular disease. Int. J. Mol. Sci. 21, 4314 (2020).","journal-title":"Int. J. Mol. Sci."},{"key":"8362_CR30","doi-asserted-by":"publisher","first-page":"H1459","DOI":"10.1152\/ajpheart.00247.2020","volume":"319","author":"T-C Cheng","year":"2020","unstructured":"Cheng, T.-C. et al. Estrogen receptor-\u03b1 prevents right ventricular diastolic dysfunction and fibrosis in female rats. Am. J. Physiol.-Heart Circ. Physiol. 319, H1459\u2013H1473 (2020).","journal-title":"Am. J. Physiol.-Heart Circ. Physiol."},{"key":"8362_CR31","doi-asserted-by":"crossref","unstructured":"Ueda, K., Adachi, Y., Liu, P., Fukuma, N. & Takimoto, E. Regulatory actions of estrogen receptor signaling in the cardiovascular system. Front Endocrinol. 10, 909 (2020).","DOI":"10.3389\/fendo.2019.00909"},{"key":"8362_CR32","doi-asserted-by":"publisher","first-page":"128668","DOI":"10.1016\/j.bmcl.2022.128668","volume":"64","author":"I Ejaz","year":"2022","unstructured":"Ejaz, I. et al. Rational design, synthesis, antiproliferative activity against MCF-7, MDA-MB-231 cells, estrogen receptors binding affinity, and computational study of indenopyrimidine-2,5-dione analogs for the treatment of breast cancer. Bioorg. Med. Chem. Lett. 64, 128668 (2022).","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"8362_CR33","doi-asserted-by":"publisher","first-page":"3549","DOI":"10.1158\/1078-0432.CCR-23-3465","volume":"30","author":"SM Gough","year":"2024","unstructured":"Gough, S. M. et al. Oral estrogen receptor PROTAC vepdegestrant (ARV-471) is highly efficacious as monotherapy and in combination with CDK4\/6 or PI3K\/mTOR pathway inhibitors in preclinical ER+ breast cancer models. Clin. Cancer Res. 30, 3549\u20133563 (2024).","journal-title":"Clin. Cancer Res."},{"key":"8362_CR34","doi-asserted-by":"publisher","first-page":"5862","DOI":"10.1021\/acs.analchem.1c00188","volume":"93","author":"A Kapara","year":"2021","unstructured":"Kapara, A. et al. Detection of estrogen receptor alpha and assessment of fulvestrant activity in MCF-7 tumor spheroids using microfluidics and SERS. Anal. Chem. 93, 5862\u20135871 (2021).","journal-title":"Anal. Chem."},{"key":"8362_CR35","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1210\/mend-2-12-1157","volume":"2","author":"M Saceda","year":"1988","unstructured":"Saceda, M. et al. Regulation of the estrogen receptor in MCF-7 cells by estradiol. Mol. Endocrinol. 2, 1157\u20131162 (1988).","journal-title":"Mol. Endocrinol."},{"key":"8362_CR36","doi-asserted-by":"publisher","first-page":"2776","DOI":"10.3390\/molecules26092776","volume":"26","author":"S Simu","year":"2021","unstructured":"Simu, S. et al. Insights into the behavior of triple-negative MDA-MB-231 breast carcinoma cells following the treatment with 17\u03b2-ethinylestradiol and levonorgestrel. Molecules 26, 2776 (2021).","journal-title":"Molecules"},{"key":"8362_CR37","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.semarthrit.2015.06.013","volume":"45","author":"YY Leung","year":"2015","unstructured":"Leung, Y. Y., Yao Hui, L. L. & Kraus, V. B. Colchicine\u2014update on mechanisms of action and therapeutic uses. Semin. Arthritis Rheum. 45, 341\u2013350 (2015).","journal-title":"Semin. Arthritis Rheum."},{"key":"8362_CR38","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1158\/1535-7163.MCT-13-0791","volume":"13","author":"E Mukhtar","year":"2014","unstructured":"Mukhtar, E., Adhami, V. M. & Mukhtar, H. Targeting microtubules by natural agents for cancer therapy. Mol. Cancer Ther. 13, 275\u2013284 (2014).","journal-title":"Mol. Cancer Ther."},{"key":"8362_CR39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2174\/1568011023354290","volume":"2","author":"MA Jordan","year":"2002","unstructured":"Jordan, M. A. Mechanism of action of antitumor drugs that interact with microtubules and tubulin. Curr. Med. Chem. Anticancer Agents 2, 1\u201317 (2002).","journal-title":"Curr. Med. Chem. Anticancer Agents"},{"key":"8362_CR40","doi-asserted-by":"crossref","unstructured":"Parmar, S. et al. Robust microtubule dynamics facilitate low-tension kinetochore detachment in metaphase. J. Cell Biol. 222, 1\u201315 (2023).","DOI":"10.1083\/jcb.202202085"},{"key":"8362_CR41","first-page":"1863","volume":"55","author":"E Aizu-Yokota","year":"1995","unstructured":"Aizu-Yokota, E., Susaki, A. & Sato, Y. Natural estrogens induce modulation of microtubules in Chinese hamster V79 cells in culture. Cancer Res. 55, 1863\u20131868 (1995).","journal-title":"Cancer Res."},{"key":"8362_CR42","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/nrc1317","volume":"4","author":"MA Jordan","year":"2004","unstructured":"Jordan, M. A. & Wilson, L. Microtubules as a target for anticancer drugs. Nat. Rev. Cancer 4, 253\u2013265 (2004).","journal-title":"Nat. Rev. Cancer"},{"key":"8362_CR43","doi-asserted-by":"publisher","first-page":"2545","DOI":"10.1161\/01.CIR.89.6.2545","volume":"89","author":"DM Gilligan","year":"1994","unstructured":"Gilligan, D. M., Quyyumi, A. A. & Cannon, R. O. Effects of physiological levels of estrogen on coronary vasomotor function in postmenopausal women. Circulation 89, 2545\u20132551 (1994).","journal-title":"Circulation"},{"key":"8362_CR44","doi-asserted-by":"publisher","first-page":"1587","DOI":"10.1007\/s11064-010-0336-7","volume":"36","author":"JA Smith","year":"2011","unstructured":"Smith, J. A., Das, A., Butler, J. T., Ray, S. K. & Banik, N. L. Estrogen or estrogen receptor agonist inhibits lipopolysaccharide induced microglial activation and death. Neurochem. Res. 36, 1587\u20131593 (2011).","journal-title":"Neurochem. Res."},{"key":"8362_CR45","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.maturitas.2007.02.025","volume":"57","author":"B Ettinger","year":"2007","unstructured":"Ettinger, B. Rationale for use of lower estrogen doses for postmenopausal hormone therapy. Maturitas 57, 81\u201384 (2007).","journal-title":"Maturitas"},{"key":"8362_CR46","doi-asserted-by":"publisher","first-page":"1795","DOI":"10.1007\/s00204-024-03723-4","volume":"98","author":"CJ Borgert","year":"2024","unstructured":"Borgert, C. J., Burgoon, L. D. & Matthews, J. C. The physiological and biochemical basis of potency thresholds modeled using human estrogen receptor alpha: implications for identifying endocrine disruptors. Arch. Toxicol. 98, 1795\u20131807 (2024).","journal-title":"Arch. Toxicol."},{"key":"8362_CR47","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1161\/CIRCULATIONAHA.114.009029","volume":"130","author":"M Sanghavi","year":"2014","unstructured":"Sanghavi, M. & Rutherford, J. D. Cardiovascular physiology of pregnancy. Circulation 130, 1003\u20131008 (2014).","journal-title":"Circulation"},{"key":"8362_CR48","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/S0091-679X(10)95013-9","volume":"95","author":"C Gell","year":"2010","unstructured":"Gell, C. et al. Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. Methods Cell Biol. 95, 221\u2013245 (2010).","journal-title":"Methods Cell Biol."},{"key":"8362_CR49","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/S1046-5928(03)00218-3","volume":"32","author":"M Castoldi","year":"2003","unstructured":"Castoldi, M. & Popov, A. V. Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer. Protein Expr. Purif. 32, 83\u201388 (2003).","journal-title":"Protein Expr. Purif."},{"key":"8362_CR50","first-page":"1245","volume":"6","author":"TA Reid","year":"2017","unstructured":"Reid, T. A., Coombes, C. & Gardner, M. K. Manipulation and quantification of microtubule lattice integrity. Biol. Open 6, 1245\u20131256 (2017).","journal-title":"Biol. Open"},{"key":"8362_CR51","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.1073\/pnas.0308205100","volume":"101","author":"M Piehl","year":"2004","unstructured":"Piehl, M., Tulu, U. S., Wadsworth, P. & Cassimeris, L. Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1. Proc. Natl Acad. Sci. USA 101, 1584\u20131588 (2004).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"8362_CR52","unstructured":"Institute, A. (2024, 2025). Allen Institute for Cell Science. (2024). https:\/\/www.allencell.org\/."},{"key":"8362_CR53","doi-asserted-by":"publisher","first-page":"bqae092","DOI":"10.1210\/endocr\/bqae092","volume":"165","author":"CH Diep","year":"2024","unstructured":"Diep, C. H. et al. Progesterone receptor signaling promotes cancer associated fibroblast mediated tumorigenicity in ER+ breast cancer. Endocrinology 165, bqae092 (2024).","journal-title":"Endocrinology"},{"key":"8362_CR54","doi-asserted-by":"publisher","first-page":"6035","DOI":"10.1073\/pnas.092504999","volume":"99","author":"V VanBuren","year":"2002","unstructured":"VanBuren, V., Odde, D. J. & Cassimeris, L. Estimates of lateral and longitudinal bond energies within the microtubule lattice. Proc. Natl Acad. Sci. USA 99, 6035\u20136040 (2002).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"8362_CR55","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1016\/j.cell.2011.06.053","volume":"146","author":"MK Gardner","year":"2011","unstructured":"Gardner, M. K. et al. Rapid microtubule self-assembly kinetics. Cell 146, 582\u2013592 (2011).","journal-title":"Cell"},{"key":"8362_CR56","doi-asserted-by":"publisher","unstructured":"Ogren, A. N. et al. Estradiol pauses microtubule growth without increased incidence of catastrophe events. Primary Datasets. https:\/\/doi.org\/10.6084\/m9.figshare.29161676.v1 (2025).","DOI":"10.6084\/m9.figshare.29161676.v1"}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08362-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08362-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08362-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T20:01:46Z","timestamp":1750276906000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-025-08362-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,18]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["8362"],"URL":"https:\/\/doi.org\/10.1038\/s42003-025-08362-8","relation":{},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,18]]},"assertion":[{"value":"19 February 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 June 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"938"}}