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Therefore, novel diagnostics that can detect <jats:italic>S. japonicum<\/jats:italic> are desirable. In this study, we have further developed and refined the \u2018SNAILS\u2019 DNA-based biosensor technology for the detection of schistosomes and have applied this to the specific detection of <jats:italic>S. japonicum<\/jats:italic>. Of the four new \u2018SNAILS\u2019 probe pairs developed, one of our optimised designs successfully detected and differentiated between genomic DNA isolated from 16 <jats:italic>S. japonicum<\/jats:italic> cercariae isolated from sites in the People\u2019s Republic of China and laboratory-derived <jats:italic>Schistosoma mansoni<\/jats:italic> cercariae.<\/jats:p>","DOI":"10.1038\/s42003-025-08773-7","type":"journal-article","created":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T16:17:47Z","timestamp":1756570667000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Species-specific detection of Schistosoma japonicum using the \u2018SNAILS\u2019 DNA-based biosensor"],"prefix":"10.1038","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7782-269X","authenticated-orcid":false,"given":"Alexander J.","family":"Webb","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin-Ping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Allan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0054-6893","authenticated-orcid":false,"given":"Richard J. 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