{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:38:02Z","timestamp":1788964682953,"version":"build-2803163510"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"DOI":"10.1038\/s42003-026-09683-y","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T10:03:25Z","timestamp":1770631405000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lysine malonylation regulates human sperm motility"],"prefix":"10.1038","volume":"9","author":[{"given":"Yimin","family":"Cheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houyang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenglin","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5028-0482","authenticated-orcid":false,"given":"Zhen","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7146-6121","authenticated-orcid":false,"given":"Tao","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,9]]},"reference":[{"key":"9683_CR1","doi-asserted-by":"publisher","first-page":"1068","DOI":"10.1016\/j.tplants.2018.09.004","volume":"23","author":"LD Vu","year":"2018","unstructured":"Vu, L. D., Gevaert, K. & De Smet, I. Protein language: post-translational modifications talking to each other. Trends Plant Sci. 23, 1068\u20131080 (2018).","journal-title":"Trends Plant Sci."},{"key":"9683_CR2","doi-asserted-by":"publisher","first-page":"qzae019","DOI":"10.1093\/gpbjnl\/qzae019","volume":"22","author":"B Hao","year":"2024","unstructured":"Hao, B. et al. Substrate and functional diversity of protein lysine post-translational modifications. Genom. Proteom. Bioinform. 22, qzae019 (2024).","journal-title":"Genom. Proteom. Bioinform."},{"key":"9683_CR3","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1186\/s13046-022-02338-w","volume":"41","author":"Y Fu","year":"2022","unstructured":"Fu, Y., Yu, J., Li, F. & Ge, S. Oncometabolites drive tumorigenesis by enhancing protein acylation: from chromosomal remodelling to nonhistone modification. J. Exp. Clin. Cancer Res. 41, 144 (2022).","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"9683_CR4","doi-asserted-by":"publisher","first-page":"e261","DOI":"10.1002\/mco2.261","volume":"4","author":"Q Zhong","year":"2023","unstructured":"Zhong, Q. et al. Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications. MedComm 4, e261 (2023).","journal-title":"MedComm"},{"key":"9683_CR5","doi-asserted-by":"publisher","first-page":"M111.012658","DOI":"10.1074\/mcp.M111.012658","volume":"10","author":"C Peng","year":"2011","unstructured":"Peng, C. et al. The first identification of lysine malonylation substrates and its regulatory enzyme. Mol. Cell Proteom. 10, M111.012658 (2011).","journal-title":"Mol. Cell Proteom."},{"key":"9683_CR6","doi-asserted-by":"publisher","first-page":"3056","DOI":"10.1074\/mcp.M115.048850","volume":"14","author":"G Colak","year":"2015","unstructured":"Colak, G. et al. Proteomic and biochemical studies of lysine malonylation suggest its malonic aciduria-associated regulatory role in mitochondrial function and fatty acid oxidation. Mol. Cell Proteom. 14, 3056\u20133071 (2015).","journal-title":"Mol. Cell Proteom."},{"key":"9683_CR7","doi-asserted-by":"publisher","first-page":"S109","DOI":"10.1074\/mcp.RA117.000541","volume":"18","author":"ZQ Shen","year":"2019","unstructured":"Shen, Z. Q. et al. Characterization of the sperm proteome and reproductive outcomes with in vitro, fertilization after a reduction in male ejaculatory abstinence period. Mol. Cell Proteom. 18, S109\u2013S117 (2019).","journal-title":"Mol. Cell Proteom."},{"key":"9683_CR8","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.mce.2007.11.015","volume":"282","author":"Y Gur","year":"2008","unstructured":"Gur, Y. & Breitbart, H. Protein synthesis in sperm: dialog between mitochondria and cytoplasm. Mol. Cell Endocrinol. 282, 45\u201355 (2008).","journal-title":"Mol. Cell Endocrinol."},{"key":"9683_CR9","doi-asserted-by":"publisher","first-page":"2597","DOI":"10.1002\/pmic.201600233","volume":"16","author":"Y Wang","year":"2016","unstructured":"Wang, Y., Wan, J., Ling, X., Liu, M. & Zhou, T. The human sperm proteome 2.0: an integrated resource for studying sperm functions at the level of posttranslational modification. Proteomics 16, 2597\u20132601 (2016).","journal-title":"Proteomics"},{"key":"9683_CR10","doi-asserted-by":"publisher","first-page":"659","DOI":"10.3390\/biology11050659","volume":"11","author":"R Serrano","year":"2022","unstructured":"Serrano, R., Garcia-Marin, L. J. & Bragado, M. J. Sperm phosphoproteome: unraveling male infertility. Biology 11, 659 (2022).","journal-title":"Biology"},{"key":"9683_CR11","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.jprot.2014.07.002","volume":"109","author":"G Sun","year":"2014","unstructured":"Sun, G. et al. Insights into the lysine acetylproteome of human sperm. J. Proteom. 109, 199\u2013211 (2014).","journal-title":"J. Proteom."},{"key":"9683_CR12","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1074\/mcp.M114.041384","volume":"14","author":"H Yu","year":"2015","unstructured":"Yu, H. et al. Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Mol. Cell Proteom. 14, 1009\u20131023 (2015).","journal-title":"Mol. Cell Proteom."},{"key":"9683_CR13","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.1093\/humrep\/dez068","volume":"34","author":"YM Cheng","year":"2019","unstructured":"Cheng, Y. M. et al. Lysine glutarylation in human sperm is associated with progressive motility. Hum. Reprod. 34, 1186\u20131194 (2019).","journal-title":"Hum. Reprod."},{"key":"9683_CR14","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1093\/humrep\/dez296","volume":"35","author":"YM Cheng","year":"2020","unstructured":"Cheng, Y. M. et al. Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility. Hum. Reprod. 35, 494\u2013503 (2020).","journal-title":"Hum. Reprod."},{"key":"9683_CR15","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.theriogenology.2020.09.023","volume":"158","author":"F Wang","year":"2020","unstructured":"Wang, F. et al. Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model. Theriogenology 158, 233\u2013238 (2020).","journal-title":"Theriogenology"},{"key":"9683_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2024.105213","volume":"303","author":"Y Tian","year":"2024","unstructured":"Tian, Y. et al. Global proteomic analyses of lysine acetylation, malonylation, succinylation, and crotonylation in human sperm reveal their involvement in male fertility. J. Proteom. 303, 105213 (2024).","journal-title":"J. Proteom."},{"key":"9683_CR17","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1093\/humupd\/dmi053","volume":"12","author":"WC Ford","year":"2006","unstructured":"Ford, W. C. Glycolysis and sperm motility: does a spoonful of sugar help the flagellum go round? Hum. Reprod. Update 12, 269\u2013274 (2006).","journal-title":"Hum. Reprod. Update"},{"key":"9683_CR18","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.896558","volume":"13","author":"Y Li","year":"2022","unstructured":"Li, Y. et al. Lysophosphatidic acid improves human sperm motility by enhancing glycolysis and activating L-type calcium channels. Front. Endocrinol. 13, 896558 (2022).","journal-title":"Front. Endocrinol."},{"key":"9683_CR19","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1093\/biolre\/ioad064","volume":"109","author":"WM Skinner","year":"2023","unstructured":"Skinner, W. M. et al. Mitochondrial uncouplers impair human sperm motility without altering ATP content. Biol. Reprod. 109, 192\u2013203 (2023).","journal-title":"Biol. Reprod."},{"key":"9683_CR20","doi-asserted-by":"publisher","first-page":"2610","DOI":"10.1016\/j.bbadis.2014.07.013","volume":"1842","author":"MG Buffone","year":"2014","unstructured":"Buffone, M. G., Wertheimer, E. V., Visconti, P. E. & Krapf, D. Central role of soluble adenylyl cyclase and cAMP in sperm physiology. Biochim. Biophys. Acta 1842, 2610\u20132620 (2014).","journal-title":"Biochim. Biophys. Acta"},{"key":"9683_CR21","doi-asserted-by":"publisher","first-page":"110953","DOI":"10.1016\/j.mce.2020.110953","volume":"516","author":"M Finkelstein","year":"2020","unstructured":"Finkelstein, M., Etkovitz, N. & Breitbart, H. Ca2+ signaling in mammalian spermatozoa. Mol. Cell Endocrinol. 516, 110953 (2020).","journal-title":"Mol. Cell Endocrinol."},{"key":"9683_CR22","doi-asserted-by":"publisher","first-page":"e1569","DOI":"10.1002\/wsbm.1569","volume":"14","author":"A Amaral","year":"2022","unstructured":"Amaral, A. Energy metabolism in mammalian sperm motility. WIREs Mech. Dis. 14, e1569 (2022).","journal-title":"WIREs Mech. Dis."},{"key":"9683_CR23","doi-asserted-by":"publisher","first-page":"102356","DOI":"10.1016\/j.ceca.2021.102356","volume":"94","author":"WM Rosencrans","year":"2021","unstructured":"Rosencrans, W. M., Rajendran, M., Bezrukov, S. M. & Rostovtseva, T. K. VDAC regulation of mitochondrial calcium flux: from channel biophysics to disease. Cell Calcium 94, 102356 (2021).","journal-title":"Cell Calcium"},{"key":"9683_CR24","doi-asserted-by":"publisher","first-page":"39206","DOI":"10.1074\/jbc.M104724200","volume":"276","author":"MJ Sampson","year":"2001","unstructured":"Sampson, M. J. et al. Immotile sperm and infertility in mice lacking mitochondrial voltage-dependent anion channel type 3. J. Biol. Chem. 276, 39206\u201339212 (2001).","journal-title":"J. Biol. Chem."},{"key":"9683_CR25","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1159\/000356657","volume":"33","author":"B Liu","year":"2014","unstructured":"Liu, B. et al. Co-incubation of human spermatozoa with anti-VDAC antibody reduced sperm motility. Cell Physiol. Biochem. 33, 142\u2013150 (2014). Epub 2014 Jan 24.","journal-title":"Cell Physiol. Biochem."},{"key":"9683_CR26","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.1093\/humrep\/der317","volume":"26","author":"TH Hereng","year":"2011","unstructured":"Hereng, T. H. et al. Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa. Hum. Reprod. 26, 3249\u20133263 (2011).","journal-title":"Hum. Reprod."},{"key":"9683_CR27","doi-asserted-by":"publisher","DOI":"10.1186\/s40246-024-00626-4","volume":"18","author":"M Hosseini","year":"2024","unstructured":"Hosseini, M. et al. Sperm epigenetics and male infertility: unraveling the molecular puzzle. Hum. Genom. 18, 57 (2024).","journal-title":"Hum. Genom."},{"key":"9683_CR28","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3758\/BF03193146","volume":"39","author":"F Faul","year":"2007","unstructured":"Faul, F., Erdfelder, E., Lang, A. G. & Buchner, A. G. Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav. Res. Methods 39, 175\u2013191 (2007).","journal-title":"Behav. Res. Methods"},{"key":"9683_CR29","doi-asserted-by":"publisher","DOI":"10.1038\/srep21336","volume":"6","author":"B Yang","year":"2016","unstructured":"Yang, B. et al. Histone acetyltransferease p300 modulates TIM4 expression in dendritic cells. Sci. Rep. 6, 21336 (2016).","journal-title":"Sci. Rep."},{"key":"9683_CR30","doi-asserted-by":"publisher","first-page":"101563","DOI":"10.1016\/j.gendis.2025.101563","volume":"12","author":"L Liu","year":"2025","unstructured":"Liu, L. et al. SIRT5 promotes the osteo-inductive potential of BMP9 by stabilizing the HIF-1\u03b1 protein in mouse embryonic fibroblasts. Genes Dis. 12, 101563 (2025).","journal-title":"Genes Dis."},{"key":"9683_CR31","doi-asserted-by":"publisher","first-page":"3591","DOI":"10.1016\/j.apsb.2025.05.018","volume":"15","author":"X Zhang","year":"2025","unstructured":"Zhang, X. et al. Melatonin receptor 1a alleviates sleep fragmentation-aggravated testicular injury in T2DM by suppression of TAB1\/TAK1 complex through FGFR1. Acta Pharm. Sin. B 15, 3591\u20133610 (2025).","journal-title":"Acta Pharm. Sin. B"},{"key":"9683_CR32","doi-asserted-by":"publisher","first-page":"627","DOI":"10.4103\/aja2024120","volume":"27","author":"J Pang","year":"2025","unstructured":"Pang, J. et al. Targeted gene silencing in mouse testicular Sertoli and Leydig cells using adeno-associated virus vectors. Asian J. Androl. 27, 627\u2013637 (2025).","journal-title":"Asian J. Androl."},{"key":"9683_CR33","doi-asserted-by":"publisher","first-page":"131470","DOI":"10.1016\/j.jhazmat.2023.131470","volume":"454","author":"W Xu","year":"2023","unstructured":"Xu, W. et al. Oral exposure to polystyrene nanoplastics reduced male fertility and even caused male infertility by inducing testicular and sperm toxicities in mice. J. Hazard Mater. 454, 131470 (2023).","journal-title":"J. Hazard Mater."},{"key":"9683_CR34","doi-asserted-by":"publisher","first-page":"2159","DOI":"10.1007\/s10311-024-01752-0","volume":"22","author":"Y Chen","year":"2024","unstructured":"Chen, Y. et al. Occurrence, toxicity and removal of polystyrene microplastics and nanoplastics in human sperm. Environ. Chem. Lett. 22, 2159\u20132165 (2024).","journal-title":"Environ. Chem. Lett."},{"key":"9683_CR35","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1093\/humrep\/dew332","volume":"32","author":"QX Zou","year":"2017","unstructured":"Zou, Q. X. et al. Diethylstilbestrol activates CatSper and disturbs progesterone actions in human spermatozoa. Hum. Reprod. 32, 290\u2013298 (2017).","journal-title":"Hum. Reprod."}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-026-09683-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-026-09683-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-026-09683-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T10:03:26Z","timestamp":1770631406000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-026-09683-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,9]]},"references-count":35,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["9683"],"URL":"https:\/\/doi.org\/10.1038\/s42003-026-09683-y","relation":{},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,9]]},"assertion":[{"value":"7 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"178"}}