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In\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    , a complex composed of SpoIIIE, SpoIIIM and PbpG maintains compartmentalization at a septal pore during hydrolysis of septal peptidoglycan (PG), by coordinating two critical functions: chromosome translocation from the mother cell to the forespore through the septal pore and preservation of septal pore stability through SpoIIIE-PG interactions and PG synthesis. Disruption of this mechanism leads to cytoplasmic leakage, failed chromosome transfer, and reduced sporulation. Here, we identify CprV (YteV) as a safeguarding factor that maintains compartmentalization in response to septal pore stability defects. Cells lacking CprV show mild defects in compartmentalization and chromosome translocation; however, in mutants experiencing septal pore instability, loss of CprV significantly worsens these defects. Consistent with this role, CprV accumulates at the septal pore in a SpoIIIE-dependent manner when chromosome translocation is impaired. Computational analyses indicate that CprV exhibits structural similarity to Alba proteins found in Eukaryotes and Archaea and is primarily conserved in the\n                    <jats:italic>Bacillales<\/jats:italic>\n                    . Collectively, our data support a model whereby CprV monitors septal pore stability to safeguard compartmentalization during sporulation, providing insight into how cells monitor compartmental integrity during cellular remodeling.\n                  <\/jats:p>","DOI":"10.1038\/s42003-026-10705-y","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:46:34Z","timestamp":1784130394000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis"],"prefix":"10.1038","volume":"9","author":[{"given":"Behzad","family":"Dehghani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4271-6731","authenticated-orcid":false,"given":"Chris L. 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