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EDC4 serves as a key scaffold for the decapping complex within P-bodies. Here, we demonstrate that N4BP1 interacts with EDC4, as well as with DCP1A, DCP2, and XRN1 - key components of 5\u2032-cap hydrolysis. Endogenous N4BP1 colocalizes with EDC4 in P-bodies, requiring both of its KH domains. Structural analysis revealed that N4BP1 contains a type-I KH fold but lacks the canonical GXXG motif required for single-stranded RNA binding. Deletion or mutation of KH domains non-canonical GXXG motifs disrupts the N4BP1-EDC4 complex. N4BP1 reduces HIV-1 transcript levels independently of its P-body localization or association with decapping components, implying the involvement of other host factors in regulating viral mRNAs. Similarly, for N4PB1-dependend negative regulation of endogenous transcripts in HaCaT keratinocytes, EDC4 is not essential. For both HIV-1 and endogenous transcripts, the reduction is associated with the activity of the NYN domain.<\/jats:p>","DOI":"10.1038\/s42003-026-10938-x","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:44:58Z","timestamp":1789667098000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["N4BP1 uses tandem KH domains to associate with EDC4 and mRNA decapping factors in P-bodies"],"prefix":"10.1038","volume":"9","author":[{"given":"Pawe\u0142","family":"Pi\u0142at","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2397-1670","authenticated-orcid":false,"given":"Ankur","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8191-3850","authenticated-orcid":false,"given":"Udo","family":"Heinemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8760-0700","authenticated-orcid":false,"given":"Mateusz","family":"Wilamowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0816-3475","authenticated-orcid":false,"given":"Jolanta","family":"Jura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"10938_CR1","doi-asserted-by":"publisher","first-page":"2897","DOI":"10.1074\/jbc.M110047200","volume":"277","author":"R Murillas","year":"2002","unstructured":"Murillas, R., Simms, K. 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