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Dual-comb spectroscopy, a comb-laser-based multi-heterodyne interferometric technique that enables simultaneous achievement of broadband, high-resolution, and rapid spectral acquisition, opens a new era of time-resolved spectroscopic measurements. Employing an electro-optic dual-comb spectrometer with central wavelength near 3\u2009\u00b5m coupled with a Herriott multipass absorption cell, here we demonstrate simultaneous determination of multiple species, including methanol, formaldehyde, HO<jats:sub>2<\/jats:sub> and OH radicals, and investigate the reaction kinetics. In addition to quantitative spectral analyses of high-resolution and tens of microsecond time-resolved spectra recorded upon flash photolysis of precursor mixtures, we determine a rate coefficient of the HO<jats:sub>2<\/jats:sub>\u2009+\u2009NO reaction by directly detecting both HO<jats:sub>2<\/jats:sub> and OH radicals. Our approach exhibits potential in discovering reactive intermediates and exploring complex reaction mechanisms, especially those of radical-radical reactions.<\/jats:p>","DOI":"10.1038\/s42004-020-00353-6","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T10:24:33Z","timestamp":1596104673000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Simultaneous determination of transient free radicals and reaction kinetics by high-resolution time-resolved dual-comb spectroscopy"],"prefix":"10.1038","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5914-5234","authenticated-orcid":false,"given":"Pei-Ling","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Er-Chien","family":"Horng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"353_CR1","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1063\/1.1142003","volume":"62","author":"CJ Manning","year":"1991","unstructured":"Manning, C. 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