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Despite the double bond (C\u2009=\u2009C) position being a crucial factor in biological system, the C\u2009=\u2009C defined structures have not yet been characterized comprehensively. Here, we present an approach for C\u2009=\u2009C position-resolved untargeted lipidomics using a combination of oxygen attachment dissociation and computational mass spectrometry to increase the annotation rate. We validated the accuracy of our platform as per the authentic standards of 85 lipids and the biogenic standards of 52 molecules containing polyunsaturated fatty acids (PUFAs) from the cultured cells fed with various fatty acid-enriched media. By analyzing human and mice-derived samples, we characterized 648 unique lipids with the C\u2009=\u2009C position-resolved level encompassing 24 lipid subclasses defined by LIPIDMAPS. Our platform also illuminated the unique profiles of tissue-specific lipids containing n-3 and\/or n-6 very long-chain PUFAs (carbon\n                    <jats:inline-formula>\n                      <jats:alternatives>\n                        <jats:tex-math>$$\\ge$$<\/jats:tex-math>\n                        <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                          <mml:mo>\u2265<\/mml:mo>\n                        <\/mml:math>\n                      <\/jats:alternatives>\n                    <\/jats:inline-formula>\n                    28 and double bonds\n                    <jats:inline-formula>\n                      <jats:alternatives>\n                        <jats:tex-math>$$\\ge$$<\/jats:tex-math>\n                        <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                          <mml:mo>\u2265<\/mml:mo>\n                        <\/mml:math>\n                      <\/jats:alternatives>\n                    <\/jats:inline-formula>\n                    4) in the eye, testis, and brain of the mouse.\n                  <\/jats:p>","DOI":"10.1038\/s42004-022-00778-1","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T06:03:15Z","timestamp":1671429795000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Computational mass spectrometry accelerates C\u2009=\u2009C position-resolved untargeted lipidomics using oxygen attachment dissociation"],"prefix":"10.1038","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1204-5436","authenticated-orcid":false,"given":"Haruki","family":"Uchino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2015-3958","authenticated-orcid":false,"given":"Hiroshi","family":"Tsugawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6887-1724","authenticated-orcid":false,"given":"Hidenori","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9902-0463","authenticated-orcid":false,"given":"Makoto","family":"Arita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,12,19]]},"reference":[{"key":"778_CR1","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1038\/nrm.2017.138","volume":"19","author":"T Harayama","year":"2018","unstructured":"Harayama, T. & Riezman, H. 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