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Here, we used the\n                    <jats:italic>dmg<\/jats:italic>\n                    biosynthetic gene cluster from\n                    <jats:italic>Amycolatopsis<\/jats:italic>\n                    sp. PS_44_ISF1 as a pathway mining probe to search for alternative demiguisin A producers. A global search identified only one other soil-derived\n                    <jats:italic>Amycolatopsis<\/jats:italic>\n                    sp. NBC_01488, encoding a highly syntenic pathway. Under feather-mimicking conditions, PS_44_ISF1 demonstrated enhanced growth and metabolite production relative to NBC_01488. Despite phylogenomic distance, both strains produced shared metabolites, while NBC_01488 yielded more abundant demiguisins, enabling the isolation of five novel derivatives characterized by two Piz-moieties with different hydroxylation patterns and glycine truncation. Two congeners displayed potent activity against the avian pathogen\n                    <jats:italic>Mycobacterium intracellulare<\/jats:italic>\n                    and moderate activity against\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    . These findings show that targeted pathway mining guided the expansion of the chemical diversity of Piz-modifications and demiguisin congeners with promising anti-\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    activity.\n                  <\/jats:p>","DOI":"10.1038\/s42004-026-02250-w","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:57:06Z","timestamp":1792267026000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted pathway mining reveals novel modified hexapeptides from the demiguisin family"],"prefix":"10.1038","volume":"9","author":[{"given":"Tanya","family":"Decker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2598-7943","authenticated-orcid":false,"given":"Marie","family":"Dayras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-5486","authenticated-orcid":false,"given":"Lena","family":"Schweizer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9700-0474","authenticated-orcid":false,"given":"Sari","family":"Rasheed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6485-8317","authenticated-orcid":false,"given":"Franziska","family":"Fries","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bulisa","family":"Iova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6095-9059","authenticated-orcid":false,"given":"Kasun H.","family":"Bodawatta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1875-9504","authenticated-orcid":false,"given":"Knud Andreas","family":"J\u00f8nsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1042-5665","authenticated-orcid":false,"given":"Rolf","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9747-3423","authenticated-orcid":false,"given":"Christine","family":"Beemelmanns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,17]]},"reference":[{"key":"2250_CR1","doi-asserted-by":"publisher","first-page":"845","DOI":"10.20506\/rst.35.3.2574","volume":"35","author":"A Contreras","year":"2016","unstructured":"Contreras, A. et al. 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