{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T19:53:11Z","timestamp":1794513191762,"version":"4.9.1"},"reference-count":65,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Cancer"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ovarian cancer (OC) is an aggressive gynecological tumor usually diagnosed with widespread metastases and ascites. Here, we depicted a single-cell landscape of the OC ecosystem with five tumor-relevant sites, including omentum metastasis and malignant ascites. Our data reveal the potential roles of ascites-enriched memory T cells as a pool for tumor-infiltrating exhausted CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and T helper 1-like cells. Moreover, tumor-enriched macrophages exhibited a preference for monocyte-derived ontogeny, whereas macrophages in ascites were more of embryonic origin. Furthermore, we characterized MAIT and dendritic cells in malignant ascites, as well as two endothelial subsets in primary tumors as predictive biomarkers for platinum-based chemotherapy response. Taken together, our study provides a global view of the female malignant ascites ecosystem and offers valuable insights for its connection with tumor tissues and paves the way for potential markers of efficacy evaluation and therapy resistance in OC.\n                  <\/jats:p>","DOI":"10.1038\/s43018-023-00599-8","type":"journal-article","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T12:01:51Z","timestamp":1690200111000},"page":"1138-1156","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":176,"title":["Single-cell analyses implicate ascites in remodeling the ecosystems of primary and metastatic tumors in ovarian cancer"],"prefix":"10.1038","volume":"4","author":[{"given":"Xiaocui","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoduan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Ginhoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0947-9137","authenticated-orcid":false,"given":"Ziyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3789-6536","authenticated-orcid":false,"given":"Zemin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8393-3719","authenticated-orcid":false,"given":"Xipeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,7,24]]},"reference":[{"key":"599_CR1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3322\/caac.21708","volume":"72","author":"RL Siegel","year":"2022","unstructured":"Siegel, R. L., Miller, K. D., Fuchs, H. E. & Jemal, A. Cancer statistics, 2022. CA Cancer J. Clin. 72, 7\u201333 (2022).","journal-title":"CA Cancer J. Clin."},{"key":"599_CR2","doi-asserted-by":"crossref","first-page":"280","DOI":"10.3322\/caac.21559","volume":"69","author":"S Lheureux","year":"2019","unstructured":"Lheureux, S., Braunstein, M. & Oza, A. M. Epithelial ovarian cancer: evolution of management in the era of precision medicine. CA Cancer J. Clin. 69, 280\u2013304 (2019).","journal-title":"CA Cancer J. Clin."},{"key":"599_CR3","doi-asserted-by":"publisher","first-page":"4335","DOI":"10.1158\/0008-5472.CAN-20-0521","volume":"80","author":"L Geistlinger","year":"2020","unstructured":"Geistlinger, L. et al. Multiomic analysis of subtype evolution and heterogeneity in high-grade serous ovarian carcinoma. Cancer Res. 80, 4335\u20134345 (2020).","journal-title":"Cancer Res."},{"key":"599_CR4","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.1016\/S0140-6736(18)32552-2","volume":"393","author":"S Lheureux","year":"2019","unstructured":"Lheureux, S., Gourley, C., Vergote, I. & Oza, A. M. Epithelial ovarian cancer. Lancet 393, 1240\u20131253 (2019).","journal-title":"Lancet"},{"key":"599_CR5","doi-asserted-by":"publisher","first-page":"256","DOI":"10.3389\/fonc.2013.00256","volume":"3","author":"N Ahmed","year":"2013","unstructured":"Ahmed, N. & Stenvers, K. L. Getting to know ovarian cancer ascites: opportunities for targeted therapy-based translational research. Front. Oncol. 3, 256 (2013).","journal-title":"Front. Oncol."},{"key":"599_CR6","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.ccr.2014.05.002","volume":"26","author":"S Pradeep","year":"2014","unstructured":"Pradeep, S. et al. Hematogenous metastasis of ovarian cancer: rethinking mode of spread. Cancer Cell 26, 77\u201391 (2014).","journal-title":"Cancer Cell"},{"key":"599_CR7","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1001\/jamaoncol.2018.6258","volume":"5","author":"ML Disis","year":"2019","unstructured":"Disis, M. L. et al. Efficacy and safety of avelumab for patients with recurrent or refractory ovarian cancer: phase 1b results from the JAVELIN solid tumor trial. JAMA Oncol. 5, 393\u2013401 (2019).","journal-title":"JAMA Oncol."},{"key":"599_CR8","doi-asserted-by":"publisher","first-page":"viii1","DOI":"10.1093\/annonc\/mdx444","volume":"28","author":"K Odunsi","year":"2017","unstructured":"Odunsi, K. Immunotherapy in ovarian cancer. Ann. Oncol. 28, viii1\u2013viii7 (2017).","journal-title":"Ann. Oncol."},{"key":"599_CR9","doi-asserted-by":"publisher","first-page":"e1277308","DOI":"10.1080\/2162402X.2016.1277308","volume":"6","author":"BE Howitt","year":"2017","unstructured":"Howitt, B. E. et al. Clear cell ovarian cancers with microsatellite instability: a unique subset of ovarian cancers with increased tumor-infiltrating lymphocytes and PD-1\/PD-L1 expression. Oncoimmunology 6, e1277308 (2017).","journal-title":"Oncoimmunology"},{"key":"599_CR10","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1038\/nrc.2017.58","volume":"17","author":"T Baslan","year":"2017","unstructured":"Baslan, T. & Hicks, J. Unravelling biology and shifting paradigms in cancer with single-cell sequencing. Nat. Rev. Cancer 17, 557\u2013569 (2017).","journal-title":"Nat. Rev. Cancer"},{"key":"599_CR11","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1038\/s43018-021-00292-8","volume":"3","author":"B Liu","year":"2022","unstructured":"Liu, B. et al. Temporal single-cell tracing reveals clonal revival and expansion of precursor exhausted T cells during anti-PD-1 therapy in lung cancer. Nat Cancer 3, 108\u2013121 (2022).","journal-title":"Nat Cancer"},{"key":"599_CR12","doi-asserted-by":"publisher","first-page":"abe6474","DOI":"10.1126\/science.abe6474","volume":"374","author":"L Zheng","year":"2021","unstructured":"Zheng, L. et al. Pan-cancer single-cell landscape of tumor-infiltrating T cells. Science 374, abe6474 (2021).","journal-title":"Science"},{"key":"599_CR13","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1016\/j.ccell.2021.04.004","volume":"39","author":"M Hornburg","year":"2021","unstructured":"Hornburg, M. et al. Single-cell dissection of cellular components and interactions shaping the tumor immune phenotypes in ovarian cancer. Cancer Cell 39, 928\u2013944 (2021).","journal-title":"Cancer Cell"},{"key":"599_CR14","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.ccell.2022.03.008","volume":"40","author":"CM Anadon","year":"2022","unstructured":"Anadon, C. M. et al. Ovarian cancer immunogenicity is governed by a narrow subset of progenitor tissue-resident memory T cells. Cancer Cell 40, 545\u2013557 (2022).","journal-title":"Cancer Cell"},{"key":"599_CR15","doi-asserted-by":"publisher","first-page":"1271","DOI":"10.1038\/s41591-020-0926-0","volume":"26","author":"B Izar","year":"2020","unstructured":"Izar, B. et al. A single-cell landscape of high-grade serous ovarian cancer. Nat. Med. 26, 1271\u20131279 (2020).","journal-title":"Nat. Med."},{"key":"599_CR16","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1158\/2159-8290.CD-11-0117","volume":"1","author":"HA Kenny","year":"2011","unstructured":"Kenny, H. A., Nieman, K. M., Mitra, A. K. & Lengyel, E. The first line of intra-abdominal metastatic attack: breaching the mesothelial cell layer. Cancer Discov. 1, 100\u2013102 (2011).","journal-title":"Cancer Discov."},{"key":"599_CR17","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1038\/nrc1611","volume":"5","author":"H Naora","year":"2005","unstructured":"Naora, H. & Montell, D. J. Ovarian cancer metastasis: integrating insights from disparate model organisms. Nat. Rev. Cancer 5, 355\u2013366 (2005).","journal-title":"Nat. Rev. Cancer"},{"key":"599_CR18","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1038\/s41586-018-0694-x","volume":"564","author":"L Zhang","year":"2018","unstructured":"Zhang, L. et al. Lineage tracking reveals dynamic relationships of T cells in colorectal cancer. Nature 564, 268\u2013272 (2018).","journal-title":"Nature"},{"key":"599_CR19","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1038\/nri3405","volume":"13","author":"L Chen","year":"2013","unstructured":"Chen, L. & Flies, D. B. Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat. Rev. Immunol. 13, 227\u2013242 (2013).","journal-title":"Nat. Rev. Immunol."},{"key":"599_CR20","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1158\/2159-8290.CD-21-0316","volume":"12","author":"Y Wu","year":"2022","unstructured":"Wu, Y. et al. Spatiotemporal immune landscape of colorectal cancer liver metastasis at single-cell level. Cancer Discov. 12, 134\u2013153 (2022).","journal-title":"Cancer Discov."},{"key":"599_CR21","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1016\/j.immuni.2019.09.013","volume":"51","author":"Z Chen","year":"2019","unstructured":"Chen, Z. et al. TCF-1-centered transcriptional network drives an effector versus exhausted CD8 T cell-fate decision. Immunity 51, 840\u2013855 (2019).","journal-title":"Immunity"},{"key":"599_CR22","doi-asserted-by":"publisher","first-page":"e20201329","DOI":"10.1084\/jem.20201329","volume":"218","author":"D Bhatt","year":"2021","unstructured":"Bhatt, D. et al. STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets. J. Exp. Med. 218, e20201329 (2021).","journal-title":"J. Exp. Med."},{"key":"599_CR23","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1663-x","volume":"20","author":"FA Wolf","year":"2019","unstructured":"Wolf, F. A. et al. PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Genome Biol. 20, 59 (2019).","journal-title":"Genome Biol"},{"key":"599_CR24","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1038\/s41587-019-0068-4","volume":"37","author":"M Setty","year":"2019","unstructured":"Setty, M. et al. Characterization of cell fate probabilities in single-cell data with Palantir. Nat. Biotechnol. 37, 451\u2013460 (2019).","journal-title":"Nat. Biotechnol."},{"key":"599_CR25","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1038\/s41591-018-0045-3","volume":"24","author":"X Guo","year":"2018","unstructured":"Guo, X. et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nat. Med. 24, 978\u2013985 (2018).","journal-title":"Nat. Med."},{"key":"599_CR26","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1038\/s41586-020-2134-y","volume":"580","author":"B Maier","year":"2020","unstructured":"Maier, B. et al. A conserved dendritic-cell regulatory program limits antitumour immunity. Nature 580, 257\u2013262 (2020).","journal-title":"Nature"},{"key":"599_CR27","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1016\/j.cell.2019.10.003","volume":"179","author":"Q Zhang","year":"2019","unstructured":"Zhang, Q. et al. Landscape and dynamics of single immune cells in hepatocellular carcinoma. Cell 179, 829\u2013845 (2019).","journal-title":"Cell"},{"key":"599_CR28","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.cell.2020.03.048","volume":"181","author":"L Zhang","year":"2020","unstructured":"Zhang, L. et al. Single-cell analyses inform mechanisms of myeloid-targeted therapies in colon cancer. Cell 181, 442\u2013459 (2020).","journal-title":"Cell"},{"key":"599_CR29","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1016\/j.immuni.2019.03.011","volume":"50","author":"ED Tait Wojno","year":"2019","unstructured":"Tait Wojno, E. D., Hunter, C. A. & Stumhofer, J. S. The immunobiology of the interleukin-12 family: room for discovery. Immunity 50, 851\u2013870 (2019).","journal-title":"Immunity"},{"key":"599_CR30","doi-asserted-by":"publisher","unstructured":"Kvedaraite, E. & Ginhoux, F. Human dendritic cells in cancer. Sci. Immunol. https:\/\/doi.org\/10.1126\/sciimmunol.abm9409 (2022).","DOI":"10.1126\/sciimmunol.abm9409"},{"key":"599_CR31","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1016\/j.cell.2005.12.039","volume":"124","author":"KY Helmy","year":"2006","unstructured":"Helmy, K. Y. et al. CRIg: a macrophage complement receptor required for phagocytosis of circulating pathogens. Cell 124, 915\u2013927 (2006).","journal-title":"Cell"},{"key":"599_CR32","doi-asserted-by":"publisher","first-page":"2988","DOI":"10.1016\/j.cell.2021.04.038","volume":"184","author":"A Obradovic","year":"2021","unstructured":"Obradovic, A. et al. Single-cell protein activity analysis identifies recurrence-associated renal tumor macrophages. Cell 184, 2988\u20133005 (2021).","journal-title":"Cell"},{"key":"599_CR33","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1158\/2159-8290.CD-20-0603","volume":"11","author":"A Dongre","year":"2021","unstructured":"Dongre, A. et al. Direct and indirect regulators of epithelial-mesenchymal transition-mediated immunosuppression in breast carcinomas. Cancer Discov. 11, 1286\u20131305 (2021).","journal-title":"Cancer Discov."},{"key":"599_CR34","doi-asserted-by":"publisher","first-page":"1317","DOI":"10.1016\/j.immuni.2019.03.009","volume":"50","author":"R Zilionis","year":"2019","unstructured":"Zilionis, R. et al. Single-cell transcriptomics of human and mouse lung cancers reveals conserved myeloid populations across individuals and species. Immunity 50, 1317\u20131334 (2019).","journal-title":"Immunity"},{"key":"599_CR35","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.1016\/j.ccell.2021.09.005","volume":"39","author":"Y Kfoury","year":"2021","unstructured":"Kfoury, Y. et al. Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment. Cancer Cell 39, 1464\u20131478 (2021).","journal-title":"Cancer Cell"},{"key":"599_CR36","doi-asserted-by":"publisher","first-page":"1764","DOI":"10.1136\/gutjnl-2018-316324","volume":"68","author":"C Lin","year":"2019","unstructured":"Lin, C. et al. Tumour-associated macrophages-derived CXCL8 determines immune evasion through autonomous PD-L1 expression in gastric cancer. Gut 68, 1764\u20131773 (2019).","journal-title":"Gut"},{"key":"599_CR37","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/nrc3893","volume":"15","author":"AR Bresnick","year":"2015","unstructured":"Bresnick, A. R., Weber, D. J. & Zimmer, D. B. S100 proteins in cancer. Nat. Rev. Cancer 15, 96\u2013109 (2015).","journal-title":"Nat. Rev. Cancer"},{"key":"599_CR38","doi-asserted-by":"publisher","first-page":"eaau0964","DOI":"10.1126\/science.aau0964","volume":"363","author":"S Chakarov","year":"2019","unstructured":"Chakarov, S. et al. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science 363, eaau0964 (2019).","journal-title":"Science"},{"key":"599_CR39","doi-asserted-by":"publisher","first-page":"e20191869","DOI":"10.1084\/jem.20191869","volume":"217","author":"A Etzerodt","year":"2020","unstructured":"Etzerodt, A. et al. Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer. J. Exp. Med. 217, e20191869 (2020).","journal-title":"J. Exp. Med."},{"key":"599_CR40","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1084\/jem.20161375","volume":"214","author":"AG N","year":"2017","unstructured":"N, A. G. et al. Phagocytosis imprints heterogeneity in tissue-resident macrophages. J. Exp. Med. 214, 1281\u20131296 (2017).","journal-title":"J. Exp. Med."},{"key":"599_CR41","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1016\/j.cell.2022.02.021","volume":"185","author":"R Nalio Ramos","year":"2022","unstructured":"Nalio Ramos, R. et al. Tissue-resident FOLR2(+) macrophages associate with CD8(+) T cell infiltration in human breast cancer. Cell 185, 1189\u20131207 (2022).","journal-title":"Cell"},{"key":"599_CR42","doi-asserted-by":"publisher","first-page":"1509","DOI":"10.1016\/j.cell.2019.08.009","volume":"178","author":"Z Liu","year":"2019","unstructured":"Liu, Z. et al. Fate mapping via Ms4a3-expression history traces monocyte-derived cells. Cell 178, 1509\u20131525 (2019).","journal-title":"Cell"},{"key":"599_CR43","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1016\/j.immuni.2016.02.024","volume":"44","author":"F Ginhoux","year":"2016","unstructured":"Ginhoux, F. & Guilliams, M. Tissue-resident macrophage ontogeny and homeostasis. Immunity 44, 439\u2013449 (2016).","journal-title":"Immunity"},{"key":"599_CR44","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1016\/j.cell.2020.07.027","volume":"182","author":"B Li","year":"2020","unstructured":"Li, B., Hao, J., Zeng, J. & Sauter, E. R. SnapShot: FABP functions. Cell 182, 1066\u20131066 (2020).","journal-title":"Cell"},{"key":"599_CR45","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.ccell.2019.06.009","volume":"36","author":"W Su","year":"2019","unstructured":"Su, W. et al. The polycomb repressor complex 1 drives double-negative prostate cancer metastasis by coordinating stemness and immune suppression. Cancer Cell 36, 139\u2013155 (2019).","journal-title":"Cancer Cell"},{"key":"599_CR46","doi-asserted-by":"publisher","first-page":"406","DOI":"10.3390\/cancers10110406","volume":"10","author":"S Dasari","year":"2018","unstructured":"Dasari, S., Fang, Y. & Mitra, A. K. Cancer associated fibroblasts: naughty neighbors that drive ovarian cancer progression. Cancers 10, 406 (2018).","journal-title":"Cancers"},{"key":"599_CR47","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1038\/s41586-020-2922-4","volume":"587","author":"KJ Travaglini","year":"2020","unstructured":"Travaglini, K. J. et al. A molecular cell atlas of the human lung from single-cell RNA sequencing. Nature 587, 619\u2013625 (2020).","journal-title":"Nature"},{"key":"599_CR48","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1158\/2159-8290.CD-16-0778","volume":"7","author":"A Patnaik","year":"2017","unstructured":"Patnaik, A. et al. Cabozantinib eradicates advanced murine prostate cancer by activating antitumor innate immunity. Cancer Discov. 7, 750\u2013765 (2017).","journal-title":"Cancer Discov."},{"key":"599_CR49","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26460-z","volume":"12","author":"Z Zheng","year":"2021","unstructured":"Zheng, Z. et al. Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3. Nat. Commun. 12, 6202 (2021).","journal-title":"Nat. Commun."},{"key":"599_CR50","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.ccell.2020.03.002","volume":"37","author":"J Goveia","year":"2020","unstructured":"Goveia, J. et al. An Integrated gene expression landscape profiling approach to identify lung tumor endothelial cell heterogeneity and angiogenic candidates. Cancer Cell 37, 421 (2020).","journal-title":"Cancer Cell"},{"key":"599_CR51","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1016\/j.cmet.2013.08.001","volume":"18","author":"K De Bock","year":"2013","unstructured":"De Bock, K., Georgiadou, M. & Carmeliet, P. Role of endothelial cell metabolism in vessel sprouting. Cell Metab. 18, 634\u2013647 (2013).","journal-title":"Cell Metab."},{"key":"599_CR52","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0381-7","volume":"15","author":"A Thiriot","year":"2017","unstructured":"Thiriot, A. et al. Differential DARC\/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues. BMC Biol. 15, 45 (2017).","journal-title":"BMC Biol."},{"key":"599_CR53","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1002\/hep.31153","volume":"72","author":"CE Niehaus","year":"2020","unstructured":"Niehaus, C. E. et al. MAIT cells are enriched and highly functional in ascites of patients with decompensated liver cirrhosis. Hepatology 72, 1378\u20131393 (2020).","journal-title":"Hepatology"},{"key":"599_CR54","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1038\/s41590-019-0444-8","volume":"20","author":"DI Godfrey","year":"2019","unstructured":"Godfrey, D. I., Koay, H. F., McCluskey, J. & Gherardin, N. A. The biology and functional importance of MAIT cells. Nat. Immunol. 20, 1110\u20131128 (2019).","journal-title":"Nat. Immunol."},{"key":"599_CR55","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21928-4","volume":"12","author":"S De Biasi","year":"2021","unstructured":"De Biasi, S. et al. Circulating mucosal-associated invariant T cells identify patients responding to anti-PD-1 therapy. Nat. Commun. 12, 1669 (2021).","journal-title":"Nat. Commun."},{"key":"599_CR56","doi-asserted-by":"publisher","first-page":"1827","DOI":"10.1038\/nm.3013","volume":"18","author":"CA Alvarez-Breckenridge","year":"2012","unstructured":"Alvarez-Breckenridge, C. A. et al. NK cells impede glioblastoma virotherapy through NKp30 and NKp46 natural cytotoxicity receptors. Nat. Med. 18, 1827\u20131834 (2012).","journal-title":"Nat. Med."},{"key":"599_CR57","doi-asserted-by":"publisher","first-page":"3987","DOI":"10.1182\/blood-2011-09-379693","volume":"119","author":"E Montaldo","year":"2012","unstructured":"Montaldo, E. et al. Human NK cells at early stages of differentiation produce CXCL8 and express CD161 molecule that functions as an activating receptor. Blood 119, 3987\u20133996 (2012).","journal-title":"Blood"},{"key":"599_CR58","doi-asserted-by":"publisher","first-page":"1179299x1986081","DOI":"10.1177\/1179299X19860815","volume":"11","author":"R Pokhriyal","year":"2019","unstructured":"Pokhriyal, R., Hariprasad, R., Kumar, L. & Hariprasad, G. Chemotherapy resistance in advanced ovarian cancer patients. Biomark. Cancer 11, 1179299x19860815 (2019).","journal-title":"Biomark. Cancer"},{"key":"599_CR59","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1016\/j.cell.2021.01.010","volume":"184","author":"S Cheng","year":"2021","unstructured":"Cheng, S. et al. A pan-cancer single-cell transcriptional atlas of tumor infiltrating myeloid cells. Cell 184, 792\u2013809 (2021).","journal-title":"Cell"},{"key":"599_CR60","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1038\/s41586-021-03651-8","volume":"595","author":"M Casanova-Acebes","year":"2021","unstructured":"Casanova-Acebes, M. et al. Tissue-resident macrophages provide a pro-tumorigenic niche to early NSCLC cells. Nature 595, 578\u2013584 (2021).","journal-title":"Nature"},{"key":"599_CR61","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1038\/s41588-021-00911-1","volume":"53","author":"SZ Wu","year":"2021","unstructured":"Wu, S. Z. et al. A single-cell and spatially resolved atlas of human breast cancers. Nat. Genet. 53, 1334\u20131347 (2021).","journal-title":"Nat. Genet."},{"key":"599_CR62","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1038\/s41587-021-01033-z","volume":"40","author":"E Dann","year":"2022","unstructured":"Dann, E., Henderson, N. C., Teichmann, S. A., Morgan, M. D. & Marioni, J. C. Differential abundance testing on single-cell data using k-nearest neighbor graphs. Nat. Biotechnol. 40, 245\u2013253 (2022).","journal-title":"Nat. Biotechnol."},{"key":"599_CR63","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1382-0","volume":"19","author":"FA Wolf","year":"2018","unstructured":"Wolf, F. A., Angerer, P. & Theis, F. J. SCANPY: large-scale single-cell gene expression data analysis. Genome Biol. 19, 15 (2018).","journal-title":"Genome Biol."},{"key":"599_CR64","doi-asserted-by":"publisher","first-page":"1484","DOI":"10.1038\/s41596-020-0292-x","volume":"15","author":"M Efremova","year":"2020","unstructured":"Efremova, M., Vento-Tormo, M., Teichmann, S. A. & Vento-Tormo, R. CellPhoneDB: inferring cell\u2013cell communication from combined expression of multi-subunit ligand\u2013receptor complexes. Nat. Protoc. 15, 1484\u20131506 (2020).","journal-title":"Nat. Protoc."},{"key":"599_CR65","doi-asserted-by":"publisher","unstructured":"Wang, X. & Zheng, X. scRNA-seq data in ovarian cancer. Mendeley Data, v.1 https:\/\/doi.org\/10.17632\/rc47y6m9mp.1 (2022).","DOI":"10.17632\/rc47y6m9mp.1"}],"container-title":["Nature Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s43018-023-00599-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s43018-023-00599-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s43018-023-00599-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,17]],"date-time":"2023-12-17T12:10:29Z","timestamp":1702815029000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s43018-023-00599-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,24]]},"references-count":65,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["599"],"URL":"https:\/\/doi.org\/10.1038\/s43018-023-00599-8","relation":{},"ISSN":["2662-1347"],"issn-type":[{"value":"2662-1347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,24]]},"assertion":[{"value":"18 March 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 June 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 July 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Z.H. is an employee of Analytical Biosciences. Z.Z. is a founder of Analytical Biosciences. All financial interests are unrelated to this study. The remaining authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}