{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:37:20Z","timestamp":1792229840762,"version":"4.4.0"},"reference-count":152,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Aging"],"DOI":"10.1038\/s43587-024-00632-0","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T06:02:27Z","timestamp":1715752947000},"page":"791-813","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["The killifish germline regulates longevity and somatic repair in a sex-specific manner"],"prefix":"10.1038","volume":"4","author":[{"given":"Eitan","family":"Moses","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6836-7659","authenticated-orcid":false,"given":"Tehila","family":"Atlan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7971-4051","authenticated-orcid":false,"given":"Xue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Fran\u011bk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atif","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgi K.","family":"Marinov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4071-5361","authenticated-orcid":false,"given":"Sagiv","family":"Shifman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Zucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adi","family":"Oron-Gottesman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Greenleaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5552-7086","authenticated-orcid":false,"given":"Ehud","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oren","family":"Ram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9749-8279","authenticated-orcid":false,"given":"Itamar","family":"Harel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"632_CR1","doi-asserted-by":"crossref","unstructured":"Ricklefs, R. E. Life-history connections to rates of aging in terrestrial vertebrates. Proc. Natl Acad. Sci. USA 107, 10314\u201310319 (2010).","DOI":"10.1073\/pnas.1005862107"},{"key":"632_CR2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1093\/gerona\/62.2.149","volume":"62","author":"JP de Magalh\u00e3es","year":"2007","unstructured":"de Magalh\u00e3es, J. P., Costa, J. & Church, G. M. An analysis of the relationship between metabolism, developmental schedules, and longevity using phylogenetic independent contrasts. J. Gerontol. A Biol. Sci. Med. Sci. 62, 149\u2013160 (2007).","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"632_CR3","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1093\/emph\/eoy033","volume":"2018","author":"SN Austad","year":"2018","unstructured":"Austad, S. N. & Hoffman, J. M. Is antagonistic pleiotropy ubiquitous in aging biology? Evol. Med. Public Health 2018, 287\u2013294 (2018).","journal-title":"Evol. Med. Public Health"},{"key":"632_CR4","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/270301a0","volume":"270","author":"TBL Kirkwood","year":"1977","unstructured":"Kirkwood, T. B. L. Evolution of ageing. Nature 270, 301\u2013304 (1977).","journal-title":"Nature"},{"key":"632_CR5","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/35041682","volume":"408","author":"TBL Kirkwood","year":"2000","unstructured":"Kirkwood, T. B. L. & Austad, S. N. Why do we age? Nature 408, 233\u2013238 (2000).","journal-title":"Nature"},{"key":"632_CR6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.tig.2007.11.004","volume":"24","author":"B Schumacher","year":"2008","unstructured":"Schumacher, B., Garinis, G. A. & Hoeijmakers, J. H. J. Age to survive: DNA damage and aging. Trends Genet. 24, 77\u201385 (2008).","journal-title":"Trends Genet."},{"key":"632_CR7","doi-asserted-by":"crossref","unstructured":"Chen, H. Y., Jolly, C., Bublys, K., Marcu, D. & Immler, S. Trade-off between somatic and germline repair in a vertebrate supports the expensive germ line hypothesis. Proc. Natl Acad. Sci. USA 117, 8973\u20138979 (2020).","DOI":"10.1073\/pnas.1918205117"},{"key":"632_CR8","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/20694","volume":"399","author":"H Hsin","year":"1999","unstructured":"Hsin, H. & Kenyon, C. Signals from the reproductive system regulate the lifespan of C. elegans. Nature 399, 362\u2013366 (1999).","journal-title":"Nature"},{"key":"632_CR9","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3389\/fmolb.2015.00003","volume":"2","author":"A Khodakarami","year":"2015","unstructured":"Khodakarami, A., Saez, I., Mels, J. & Vilchez, D. Mediation of organismal aging and somatic proteostasis by the germline. Front. Mol. Biosci. 2, 3 (2015).","journal-title":"Front. Mol. Biosci."},{"key":"632_CR10","doi-asserted-by":"crossref","unstructured":"Hamilton, J. B. & Mestler, G. E. Mortality and survival: comparison of eunuchs with intact men and women in a mentally retarded population. J. Gerontol. 24, 395\u2013411 (1969).","DOI":"10.1093\/geronj\/24.4.395"},{"key":"632_CR11","doi-asserted-by":"crossref","first-page":"R792","DOI":"10.1016\/j.cub.2012.06.036","volume":"22","author":"KJ Min","year":"2012","unstructured":"Min, K. J., Lee, C. K. & Park, H. N. The lifespan of Korean eunuchs. Curr. Biol. 22, R792\u2013R793 (2012).","journal-title":"Curr. Biol."},{"key":"632_CR12","doi-asserted-by":"crossref","first-page":"10801","DOI":"10.18632\/oncotarget.2984","volume":"6","author":"V Benedusi","year":"2015","unstructured":"Benedusi, V. et al. Ovariectomy shortens the life span of female mice. Oncotarget 6, 10801 (2015).","journal-title":"Oncotarget"},{"key":"632_CR13","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1530\/jrf.0.0140113","volume":"14","author":"SA Asdell","year":"1967","unstructured":"Asdell, S. A., Doornenbal, H., Joshi, S. R. & Sperling, G. A. The effects of sex steroid hormones upon longevity in rats. Reproduction 14, 113\u2013120 (1967).","journal-title":"Reproduction"},{"key":"632_CR14","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1046\/j.1474-9728.2003.00049.x","volume":"2","author":"SL Cargill","year":"2003","unstructured":"Cargill, S. L., Carey, J. R., M\u00fcller, H. G. & Anderson, G. Age of ovary determines remaining life expectancy in old ovariectomized mice. Aging Cell 2, 185\u2013190 (2003).","journal-title":"Aging Cell"},{"key":"632_CR15","doi-asserted-by":"crossref","first-page":"e61082","DOI":"10.1371\/journal.pone.0061082","volume":"8","author":"JM Hoffman","year":"2013","unstructured":"Hoffman, J. M., Creevy, K. E. & Promislow, D. E. L. Reproductive capability is associated with lifespan and cause of death in companion dogs. PLoS ONE 8, e61082 (2013).","journal-title":"PLoS ONE"},{"key":"632_CR16","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1093\/geronj\/20.1.96","volume":"20","author":"JB Hamilton","year":"1965","unstructured":"Hamilton, J. B. Relationship of castration, spaying, and sex to survival and duration of life in domestic cats. J. Gerontol. 20, 96\u2013104 (1965).","journal-title":"J. Gerontol."},{"key":"632_CR17","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrendo.2013.203","volume":"10","author":"S Torre","year":"2014","unstructured":"Torre, S., Della, Benedusi, V., Fontana, R. & Maggi, A. Energy metabolism and fertility\u2014a balance preserved for female health. Nat. Rev. Endocrinol. 10, 13\u201323 (2014).","journal-title":"Nat. Rev. Endocrinol."},{"key":"632_CR18","doi-asserted-by":"crossref","first-page":"688788","DOI":"10.3389\/fcell.2021.688788","volume":"9","author":"D Gems","year":"2021","unstructured":"Gems, D., Kern, C. C., Nour, J. & Ezcurra, M. Reproductive suicide: similar mechanisms of aging in C. elegans and Pacific salmon. Front. Cell Dev. Biol. 9, 688788 (2021).","journal-title":"Front. Cell Dev. Biol."},{"key":"632_CR19","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.exger.2012.09.009","volume":"48","author":"A Antebi","year":"2013","unstructured":"Antebi, A. Regulation of longevity by the reproductive system. Exp. Gerontol. 48, 596\u2013602 (2013).","journal-title":"Exp. Gerontol."},{"key":"632_CR20","doi-asserted-by":"crossref","first-page":"16010","DOI":"10.1038\/npjamd.2016.10","volume":"2","author":"M Uno","year":"2016","unstructured":"Uno, M. & Nishida, E. Lifespan-regulating genes in C. elegans. NPJ Aging Mech. Dis. 2, 16010 (2016).","journal-title":"NPJ Aging Mech. Dis."},{"key":"632_CR21","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1038\/nature12452","volume":"501","author":"MA Ermolaeva","year":"2013","unstructured":"Ermolaeva, M. A. et al. DNA damage in germ cells induces an innate immune response that triggers systemic stress resistance. Nature 501, 416\u2013420 (2013).","journal-title":"Nature"},{"key":"632_CR22","doi-asserted-by":"crossref","first-page":"eabg3012","DOI":"10.1126\/sciadv.abg3012","volume":"7","author":"G Calculli","year":"2021","unstructured":"Calculli, G. et al. Systemic regulation of mitochondria by germline proteostasis prevents protein aggregation in the soma of C. elegans. Sci. Adv. 7, eabg3012 (2021).","journal-title":"Sci. Adv."},{"key":"632_CR23","doi-asserted-by":"crossref","unstructured":"Austad, S. N. Sex differences in longevity and aging. In Handbook of the Biology of Aging 7th edn (eds Masoro, E. J. & Austad, S. N.) 479\u2013495 (2011).","DOI":"10.1016\/B978-0-12-378638-8.00023-3"},{"key":"632_CR24","doi-asserted-by":"crossref","first-page":"D1083","DOI":"10.1093\/nar\/gkx1042","volume":"46","author":"R Tacutu","year":"2018","unstructured":"Tacutu, R. et al. Human Ageing Genomic Resources: new and updated databases. Nucleic Acids Res. 46, D1083\u2013D1090 (2018).","journal-title":"Nucleic Acids Res."},{"key":"632_CR25","first-page":"181","volume":"7","author":"M Schartl","year":"2014","unstructured":"Schartl, M. Beyond the zebrafish: diverse fish species for modeling human disease. Dis. Model. Mech. 7, 181\u2013192 (2014).","journal-title":"Dis. Model. Mech."},{"key":"632_CR26","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1126\/science.aaf1703","volume":"353","author":"J Nielsen","year":"2016","unstructured":"Nielsen, J. et al. Eye lens radiocarbon reveals centuries of longevity in the Greenland shark (Somniosus microcephalus). Science 353, 702\u2013704 (2016).","journal-title":"Science"},{"key":"632_CR27","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1126\/science.abg5332","volume":"374","author":"SRR Kolora","year":"2021","unstructured":"Kolora, S. R. R. et al. Origins and evolution of extreme life span in Pacific Ocean rockfishes. Science 374, 842\u2013847 (2021).","journal-title":"Science"},{"key":"632_CR28","doi-asserted-by":"crossref","first-page":"R822","DOI":"10.1016\/j.cub.2018.06.031","volume":"28","author":"M Vrt\u00edlek","year":"2018","unstructured":"Vrt\u00edlek, M., \u017d\u00e1k, J., P\u0161eni\u010dka, M. & Reichard, M. Extremely rapid maturation of a wild African annual fish. Curr. Biol. 28, R822\u2013R824 (2018).","journal-title":"Curr. Biol."},{"key":"632_CR29","doi-asserted-by":"crossref","unstructured":"Harel, I. The turquoise killifish. Nat. Methods 19, 1150\u20131151 (2022).","DOI":"10.1038\/s41592-022-01631-y"},{"key":"632_CR30","doi-asserted-by":"crossref","unstructured":"Harel, I. et al. A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate. Cell 160, 1013\u20131026 (2015).","DOI":"10.1016\/j.cell.2015.01.038"},{"key":"632_CR31","doi-asserted-by":"crossref","unstructured":"Astre, G., Moses, E. & Harel, I. The African turquoise killifish (Nothobranchius furzeri): biology and research applications. In Laboratory Fish in Biomedical Research (eds D\u02bcAngelo, L. & de Girolamo, P.) 245\u2013287 (Academic Press, 2021).","DOI":"10.1016\/B978-0-12-821099-4.00011-0"},{"key":"632_CR32","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/j.devcel.2023.05.015","volume":"58","author":"G Astre","year":"2023","unstructured":"Astre, G. et al. Genetic perturbation of AMP biosynthesis extends lifespan and restores metabolic health in a naturally short-lived vertebrate. Dev. Cell 58, 1350\u20131364 (2023).","journal-title":"Dev. Cell"},{"key":"632_CR33","doi-asserted-by":"crossref","unstructured":"Harel, I., Valenzano, D. R. & Brunet, A. Efficient genome engineering approaches for the short-lived African turquoise killifish. Nat. Protoc. 11, 2010\u20132028 (2016).","DOI":"10.1038\/nprot.2016.103"},{"key":"632_CR34","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1016\/j.cell.2015.10.071","volume":"163","author":"K Reichwald","year":"2015","unstructured":"Reichwald, K. et al. Insights into sex chromosome evolution and aging from the genome of a short-lived fish. Cell 163, 1527\u20131538 (2015).","journal-title":"Cell"},{"key":"632_CR35","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1016\/j.cell.2015.11.008","volume":"163","author":"DR Valenzano","year":"2015","unstructured":"Valenzano, D. R. et al. The African turquoise killifish genome provides insights into evolution and genetic architecture of lifespan. Cell 163, 1539\u20131554 (2015).","journal-title":"Cell"},{"key":"632_CR36","doi-asserted-by":"crossref","unstructured":"Rozenberg, I., Moses, E. & Harel, I. CRISPR\u2013Cas9 genome editing in Nothobranchius furzeri for gene knockout and knock-in. Cold Spring Harb. Protoc. 2023, 90\u201399 (2023).","DOI":"10.1101\/pdb.prot107742"},{"key":"632_CR37","doi-asserted-by":"crossref","first-page":"eaaz3090","DOI":"10.1126\/science.aaz3090","volume":"369","author":"W Wang","year":"2020","unstructured":"Wang, W. et al. Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates. Science 369, eaaz3090 (2020).","journal-title":"Science"},{"key":"632_CR38","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.85960","volume":"12","author":"E Moses","year":"2023","unstructured":"Moses, E., Franek, R. & Harel, I. A scalable and tunable platform for functional interrogation of peptide hormones in fish. eLife 12, e85960 (2023).","journal-title":"eLife"},{"key":"632_CR39","doi-asserted-by":"crossref","unstructured":"Slanchev, K., Stebler, J., de la Cueva-Mendez, G. & Raz, E. Development without germ cells: the role of the germ line in zebrafish sex differentiation. Proc. Natl Acad. Sci. USA 102, 4074\u20134079 (2005).","DOI":"10.1073\/pnas.0407475102"},{"key":"632_CR40","doi-asserted-by":"crossref","first-page":"16958","DOI":"10.1073\/pnas.0609932104","volume":"104","author":"H Kurokawa","year":"2007","unstructured":"Kurokawa, H. et al. Germ cells are essential for sexual dimorphism in the medaka gonad. Proc. Natl Acad. Sci. USA 104, 16958\u201316963 (2007).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"632_CR41","doi-asserted-by":"crossref","unstructured":"Kossack, M. E. & Draper, B. W. Genetic regulation of sex determination and maintenance in zebrafish (Danio rerio). In Current Topics in Developmental Biology (ed Capel, B.) 134, 119\u2013149 (Elsevier, 2019).","DOI":"10.1016\/bs.ctdb.2019.02.004"},{"key":"632_CR42","volume":"11","author":"Y Liu","year":"2022","unstructured":"Liu, Y. et al. Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary. eLife 11, e76014 (2022).","journal-title":"eLife"},{"key":"632_CR43","doi-asserted-by":"crossref","first-page":"851719","DOI":"10.3389\/fgene.2022.851719","volume":"13","author":"P Qian","year":"2022","unstructured":"Qian, P., Kang, J., Liu, D. & Xie, G. Single cell transcriptome sequencing of zebrafish testis revealed novel spermatogenesis marker genes and stronger Leydig-germ cell paracrine interactions. Front. Genet. 13, 851719 (2022).","journal-title":"Front. Genet."},{"key":"632_CR44","doi-asserted-by":"crossref","first-page":"100794","DOI":"10.1016\/j.xpro.2021.100794","volume":"2","author":"X Sun","year":"2021","unstructured":"Sun, X. et al. CloneSeq\u2014single-cell clonal 3D culture and analysis protocol. STAR Protoc. 2, 100794 (2021).","journal-title":"STAR Protoc."},{"key":"632_CR45","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.cell.2015.04.044","volume":"161","author":"AM Klein","year":"2015","unstructured":"Klein, A. M. et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 161, 1187\u20131201 (2015).","journal-title":"Cell"},{"key":"632_CR46","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1038\/s41592-019-0619-0","volume":"16","author":"I Korsunsky","year":"2019","unstructured":"Korsunsky, I. et al. Fast, sensitive and accurate integration of single-cell data with Harmony. Nat. Methods 16, 1289\u20131296 (2019).","journal-title":"Nat. Methods"},{"key":"632_CR47","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1038\/s41590-020-0736-z","volume":"21","author":"X Xie","year":"2020","unstructured":"Xie, X. et al. Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection. Nat. Immunol. 21, 1119\u20131133 (2020).","journal-title":"Nat. Immunol."},{"key":"632_CR48","doi-asserted-by":"crossref","first-page":"798712","DOI":"10.3389\/fimmu.2022.798712","volume":"13","author":"JTH Chan","year":"2022","unstructured":"Chan, J. T. H., Kadri, S., K\u00f6llner, B., Rebl, A. & Koryt\u00e1\u0159, T. RNA-seq of single fish cells\u2014seeking out the leukocytes mediating immunity in teleost fishes. Front. Immunol. 13, 798712 (2022).","journal-title":"Front. Immunol."},{"key":"632_CR49","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.1016\/j.celrep.2019.02.069","volume":"26","author":"I St\u00e9vant","year":"2019","unstructured":"St\u00e9vant, I. et al. Dissecting cell lineage specification and sex fate determination in gonadal somatic cells using single-cell transcriptomics. Cell Rep. 26, 3272\u20133283 (2019).","journal-title":"Cell Rep."},{"key":"632_CR50","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.stem.2017.03.007","volume":"20","author":"L Li","year":"2017","unstructured":"Li, L. et al. Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions. Cell Stem Cell 20, 858\u2013873 (2017).","journal-title":"Cell Stem Cell"},{"key":"632_CR51","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.3389\/fcell.2020.616387","volume":"8","author":"MA Estermann","year":"2020","unstructured":"Estermann, M. A., Major, A. T. & Smith, C. A. Gonadal sex differentiation: supporting versus steroidogenic cell lineage specification in mammals and birds. Front. Cell Dev. Biol. 8, 1711 (2020).","journal-title":"Front. Cell Dev. Biol."},{"key":"632_CR52","doi-asserted-by":"crossref","first-page":"eabh2169","DOI":"10.1126\/sciadv.abh2169","volume":"7","author":"M Karlsson","year":"2021","unstructured":"Karlsson, M. et al. A single\u2013cell type transcriptomics map of human tissues. Sci. Adv. 7, eabh2169 (2021).","journal-title":"Sci. Adv."},{"key":"632_CR53","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.fsi.2013.05.007","volume":"35","author":"S-F Niu","year":"2013","unstructured":"Niu, S.-F. et al. Characterization of a novel piscidin-like antimicrobial peptide from Pseudosciaena crocea and its immune response to Cryptocaryon irritans. Fish Shellfish Immunol. 35, 513\u2013524 (2013).","journal-title":"Fish Shellfish Immunol."},{"key":"632_CR54","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.3390\/ani11051365","volume":"11","author":"T Yan","year":"2021","unstructured":"Yan, T. et al. Estradiol upregulates the expression of the TGF-\u03b2 receptors ALK5 and BMPR2 during the gonadal development of Schizothorax prenanti. Animals 11, 1365 (2021).","journal-title":"Animals"},{"key":"632_CR55","volume":"10","author":"X Fan","year":"2019","unstructured":"Fan, X. et al. Single-cell reconstruction of follicular remodeling in the human adult ovary. Nat. Commun. 10, 3164 (2019).","journal-title":"Nat. Commun."},{"key":"632_CR56","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1210\/er.2018-00010","volume":"39","author":"E Rotgers","year":"2018","unstructured":"Rotgers, E., J\u00f8rgensen, A. & Yao, H. H.-C. At the crossroads of fate\u2014somatic cell lineage specification in the fetal gonad. Endocr. Rev. 39, 739\u2013759 (2018).","journal-title":"Endocr. Rev."},{"key":"632_CR57","doi-asserted-by":"crossref","first-page":"3157","DOI":"10.1242\/dev.124.16.3157","volume":"124","author":"C Yoon","year":"1997","unstructured":"Yoon, C., Kawakami, K. & Hopkins, N. Zebrafish vasa homologue RNA is localized to the cleavage planes of 2-and 4-cell-stage embryos and is expressed in the primordial germ cells. Development 124, 3157\u20133165 (1997).","journal-title":"Development"},{"key":"632_CR58","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1159\/000364924","volume":"8","author":"T Nishimura","year":"2014","unstructured":"Nishimura, T. & Tanaka, M. Gonadal development in fish. Sex. Dev. 8, 252\u2013261 (2014).","journal-title":"Sex. Dev."},{"key":"632_CR59","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.ygcen.2009.02.013","volume":"165","author":"RW Schulz","year":"2010","unstructured":"Schulz, R. W. et al. Spermatogenesis in fish. Gen. Comp. Endocrinol. 165, 390\u2013411 (2010).","journal-title":"Gen. Comp. Endocrinol."},{"key":"632_CR60","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ygcen.2009.05.022","volume":"165","author":"E Lubzens","year":"2010","unstructured":"Lubzens, E., Young, G., Bobe, J. & Cerd\u00e0, J. Oogenesis in teleosts: how fish eggs are formed. Gen. Comp. Endocrinol. 165, 367\u2013389 (2010).","journal-title":"Gen. Comp. Endocrinol."},{"key":"632_CR61","doi-asserted-by":"crossref","unstructured":"Li, J. & Cheng, C. H. K. Evolution of gonadotropin signaling on gonad development: insights from gene knockout studies in zebrafish. Biol. Reprod. 99, 686\u2013694 (2018).","DOI":"10.1093\/biolre\/ioy101"},{"key":"632_CR62","doi-asserted-by":"crossref","unstructured":"Liu, Y. & Lin, H. Genetic analysis of the reproductive axis in fish using genome-editing nucleases. Sci. Bull. (Beijing) 62, 302\u2013308 (2017).","DOI":"10.1016\/j.scib.2017.01.002"},{"key":"632_CR63","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/faf.12043","volume":"16","author":"RS McBride","year":"2015","unstructured":"McBride, R. S. et al. Energy acquisition and allocation to egg production in relation to fish reproductive strategies. Fish Fisheries 16, 23\u201357 (2015).","journal-title":"Fish Fisheries"},{"key":"632_CR64","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1126\/science.1181369","volume":"326","author":"E Lieberman-Aiden","year":"2009","unstructured":"Lieberman-Aiden, E. et al. Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science 326, 289\u2013293 (2009).","journal-title":"Science"},{"key":"632_CR65","doi-asserted-by":"crossref","unstructured":"Weidinger, G. et al. dead end, a novel vertebrate germ plasm component, is required for zebrafish primordial germ cell migration and survival. Curr. Biol. 13, 1429\u20131434 (2003).","DOI":"10.1016\/S0960-9822(03)00537-2"},{"key":"632_CR66","volume":"10","author":"J \u017d\u00e1k","year":"2020","unstructured":"\u017d\u00e1k, J., Dykov\u00e1, I. & Reichard, M. Good performance of turquoise killifish (Nothobranchius furzeri) on pelleted diet as a step towards husbandry standardization. Sci. Rep. 10, 8986 (2020).","journal-title":"Sci. Rep."},{"key":"632_CR67","doi-asserted-by":"crossref","first-page":"21071","DOI":"10.25225\/jvb.21074","volume":"71","author":"I Dykov\u00e1","year":"2022","unstructured":"Dykov\u00e1, I. et al. Histology of major organ systems of Nothobranchius fishes: short-lived model species. J. Vertebr. Biol. 71, 21071\u201321074 (2022).","journal-title":"J. Vertebr. Biol."},{"key":"632_CR68","first-page":"797","volume":"10","author":"JEN Minchin","year":"2017","unstructured":"Minchin, J. E. N. & Rawls, J. F. A classification system for zebrafish adipose tissues. Dis. Model. Mech. 10, 797\u2013809 (2017).","journal-title":"Dis. Model. Mech."},{"key":"632_CR69","doi-asserted-by":"crossref","first-page":"6934","DOI":"10.1038\/ncomms7934","volume":"6","author":"C Liu","year":"2015","unstructured":"Liu, C., Peng, J., Matzuk, M. M. & Yao, H. H.-C. Lineage specification of ovarian theca cells requires multicellular interactions via oocyte and granulosa cells. Nat. Commun. 6, 6934 (2015).","journal-title":"Nat. Commun."},{"key":"632_CR70","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.neures.2018.10.003","volume":"139","author":"S Koley","year":"2019","unstructured":"Koley, S., Rozenbaum, M., Fainzilber, M. & Terenzio, M. Translating regeneration: local protein synthesis in the neuronal injury response. Neurosci. Res. 139, 26\u201336 (2019).","journal-title":"Neurosci. Res."},{"key":"632_CR71","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nature18282","volume":"534","author":"S Blanco","year":"2016","unstructured":"Blanco, S. et al. Stem cell function and stress response are controlled by protein synthesis. Nature 534, 335\u2013340 (2016).","journal-title":"Nature"},{"key":"632_CR72","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1440-1746.2010.06539.x","volume":"26","author":"KJ Riehle","year":"2011","unstructured":"Riehle, K. J., Dan, Y. Y., Campbell, J. S. & Fausto, N. New concepts in liver regeneration. J. Gastroenterol. Hepatol. 26, 203\u2013212 (2011).","journal-title":"J. Gastroenterol. Hepatol."},{"key":"632_CR73","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1038\/s41594-020-00513-1","volume":"27","author":"S Wang","year":"2020","unstructured":"Wang, S., Meyer, D. H. & Schumacher, B. H3K4me2 regulates the recovery of protein biosynthesis and homeostasis following DNA damage. Nat. Struct. Mol. Biol. 27, 1165\u20131177 (2020).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"632_CR74","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.cell.2013.05.039","volume":"153","author":"C Lopez-Otin","year":"2013","unstructured":"Lopez-Otin, C. et al. The hallmarks of aging. Cell 153, 1194\u20131217 (2013).","journal-title":"Cell"},{"key":"632_CR75","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.cmet.2019.06.018","volume":"30","author":"A Tyshkovskiy","year":"2019","unstructured":"Tyshkovskiy, A. et al. Identification and application of gene expression signatures associated with lifespan extension. Cell Metab 30, 573\u2013593 (2019).","journal-title":"Cell Metab"},{"key":"632_CR76","doi-asserted-by":"crossref","unstructured":"Shi, C. & Murphy, C. T. Sex and death. In Current Topics in Developmental Biology (eds Jarriault, S. & Podbilewicz, B.) 144, 353\u2013375 (Elsevier, 2021).","DOI":"10.1016\/bs.ctdb.2020.08.004"},{"key":"632_CR77","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.mad.2004.07.003","volume":"125","author":"C Wang","year":"2004","unstructured":"Wang, C., Li, Q., Redden, D. T., Weindruch, R. & Allison, D. B. Statistical methods for testing effects on \u2018maximum lifespan\u2019. Mech. Ageing Dev. 125, 629\u2013632 (2004).","journal-title":"Mech. Ageing Dev."},{"key":"632_CR78","doi-asserted-by":"crossref","first-page":"56147","DOI":"10.18632\/oncotarget.11269","volume":"7","author":"SK Han","year":"2016","unstructured":"Han, S. K. et al. OASIS 2: online application for survival analysis 2 with features for the analysis of maximal lifespan and healthspan in aging research. Oncotarget 7, 56147 (2016).","journal-title":"Oncotarget"},{"key":"632_CR79","doi-asserted-by":"crossref","DOI":"10.1111\/acel.12786","volume":"17","author":"M Garratt","year":"2018","unstructured":"Garratt, M. et al. Male lifespan extension with 17\u2010\u03b1 estradiol is linked to a sex\u2010specific metabolomic response modulated by gonadal hormones in mice. Aging Cell 17, e12786 (2018).","journal-title":"Aging Cell"},{"key":"632_CR80","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/gerona\/glv309","volume":"72","author":"MB Stout","year":"2017","unstructured":"Stout, M. B. et al. 17\u03b1-Estradiol alleviates age-related metabolic and inflammatory dysfunction in male mice without inducing feminization. J. Gerontol. Ser. A Biomed. Sci. Med. Sci. 72, 3\u201315 (2017).","journal-title":"J. Gerontol. Ser. A Biomed. Sci. Med. Sci."},{"key":"632_CR81","first-page":"807","volume":"32","author":"M Blencowe","year":"2022","unstructured":"Blencowe, M. et al. Relative contributions of sex hormones, sex chromosomes, and gonads to sex differences in tissue gene regulation. Genome Res. 32, 807\u2013824 (2022).","journal-title":"Genome Res."},{"key":"632_CR82","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1126\/science.abn9886","volume":"378","author":"J Nikkanen","year":"2022","unstructured":"Nikkanen, J. et al. An evolutionary trade-off between host immunity and metabolism drives fatty liver in male mice. Science 378, 290\u2013295 (2022).","journal-title":"Science"},{"key":"632_CR83","volume":"9","author":"SN Mann","year":"2020","unstructured":"Mann, S. N. et al. Health benefits attributed to 17\u03b1-estradiol, a lifespan-extending compound, are mediated through estrogen receptor \u03b1. eLife 9, e59616 (2020).","journal-title":"eLife"},{"key":"632_CR84","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1111\/acel.12656","volume":"16","author":"M Garratt","year":"2017","unstructured":"Garratt, M., Bower, B., Garcia, G. G. & Miller, R. A. Sex differences in lifespan extension with acarbose and 17\u2010\u03b1 estradiol: gonadal hormones underlie male\u2010specific improvements in glucose tolerance and mTORC 2 signaling. Aging Cell 16, 1256\u20131266 (2017).","journal-title":"Aging Cell"},{"key":"632_CR85","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1126\/science.1065768","volume":"295","author":"N Arantes-Oliveira","year":"2002","unstructured":"Arantes-Oliveira, N., Apfeld, J., Dillin, A. & Kenyon, C. Regulation of life-span by germ-line stem cells in Caenorhabditis elegans. Science 295, 502\u2013505 (2002).","journal-title":"Science"},{"key":"632_CR86","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1038\/s43587-023-00521-y","volume":"3","author":"R Ripa","year":"2023","unstructured":"Ripa, R. et al. Refeeding-associated AMPK\u03b31 complex activity is a hallmark of health and longevity. Nat. Aging 3, 1544\u20131560 (2023).","journal-title":"Nat. Aging"},{"key":"632_CR87","doi-asserted-by":"crossref","first-page":"D445","DOI":"10.1093\/nar\/gkz862","volume":"48","author":"R Caspi","year":"2020","unstructured":"Caspi, R. et al. The MetaCyc database of metabolic pathways and enzymes\u2014a 2019 update. Nucleic Acids Res. 48, D445\u2013D453 (2020).","journal-title":"Nucleic Acids Res."},{"key":"632_CR88","doi-asserted-by":"crossref","first-page":"W388","DOI":"10.1093\/nar\/gkab382","volume":"49","author":"Z Pang","year":"2021","unstructured":"Pang, Z. et al. MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights. Nucleic Acids Res. 49, W388\u2013W396 (2021).","journal-title":"Nucleic Acids Res."},{"key":"632_CR89","doi-asserted-by":"crossref","first-page":"e4096","DOI":"10.1002\/nbm.4096","volume":"32","author":"MH Lee","year":"2019","unstructured":"Lee, M. H., Malloy, C. R., Corbin, I. R., Li, J. & Jin, E. S. Assessing the pentose phosphate pathway using [2, 3\u201013C2]glucose. NMR Biomed. 32, e4096 (2019).","journal-title":"NMR Biomed."},{"key":"632_CR90","doi-asserted-by":"crossref","first-page":"e1006695","DOI":"10.1371\/journal.pgen.1006695","volume":"13","author":"CF Bennett","year":"2017","unstructured":"Bennett, C. F. et al. Transaldolase inhibition impairs mitochondrial respiration and induces a starvation-like longevity response in Caenorhabditis elegans. PLoS Genet. 13, e1006695 (2017).","journal-title":"PLoS Genet."},{"key":"632_CR91","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.tree.2006.10.008","volume":"22","author":"LG Harshman","year":"2007","unstructured":"Harshman, L. G. & Zera, A. J. The cost of reproduction: the devil in the details. Trends Ecol. Evol. 22, 80\u201386 (2007).","journal-title":"Trends Ecol. Evol."},{"key":"632_CR92","doi-asserted-by":"crossref","first-page":"20191604","DOI":"10.1098\/rspb.2019.1604","volume":"286","author":"AA Maklakov","year":"2019","unstructured":"Maklakov, A. A. & Chapman, T. Evolution of ageing as a tangle of trade-offs: energy versus function. Proc. R. Soc. B 286, 20191604 (2019).","journal-title":"Proc. R. Soc. B"},{"key":"632_CR93","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/S0169-5347(00)02032-2","volume":"16","author":"AM Leroi","year":"2001","unstructured":"Leroi, A. M. Molecular signals versus the Loi de Balancement. Trends Ecol. Evol. 16, 24\u201329 (2001).","journal-title":"Trends Ecol. Evol."},{"key":"632_CR94","doi-asserted-by":"crossref","unstructured":"Valenzano, D. R., Terzibasi, E., Cattaneo, A., Domenici, L. & Cellerino, A. Temperature affects longevity and age-related locomotor and cognitive decay in the short-lived fish: Nothobranchius furzeri. Aging Cell 5, 275\u2013278 (2006).","DOI":"10.1111\/j.1474-9726.2006.00212.x"},{"key":"632_CR95","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1095-8312.2010.01406.x","volume":"100","author":"M Reichard","year":"2010","unstructured":"Reichard, M. & POLA\u010cIK, M. Reproductive isolating barriers between colour-differentiated populations of an African annual killifish, Nothobranchius korthausae (Cyprinodontiformes). Biol. J. Linn. Soc. 100, 62\u201372 (2010).","journal-title":"Biol. J. Linn. Soc."},{"key":"632_CR96","doi-asserted-by":"crossref","unstructured":"List, E. O. et al. Endocrine parameters and phenotypes of the growth hormone receptor gene disrupted (GHR\u2212\/\u2212) mouse. Endocr. Rev. 32, 356\u2013386 (2011).","DOI":"10.1210\/er.2010-0009"},{"key":"632_CR97","doi-asserted-by":"crossref","DOI":"10.1111\/acel.13656","volume":"21","author":"S Aleksic","year":"2022","unstructured":"Aleksic, S. et al. Integrity of hypothalamic\u2013pituitary\u2013testicular axis in exceptional longevity. Aging Cell 21, e13656 (2022).","journal-title":"Aging Cell"},{"key":"632_CR98","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/acel.12170","volume":"13","author":"DE Harrison","year":"2014","unstructured":"Harrison, D. E. et al. Acarbose, 17\u2010\u03b1\u2010estradiol, and nordihydroguaiaretic acid extend mouse lifespan preferentially in males. Aging Cell 13, 273\u2013282 (2014).","journal-title":"Aging Cell"},{"key":"632_CR99","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1641\/0006-3568(2005)055[0039:MUTCOE]2.0.CO;2","volume":"55","author":"TD Williams","year":"2005","unstructured":"Williams, T. D. Mechanisms underlying the costs of egg production. Bioscience 55, 39\u201348 (2005).","journal-title":"Bioscience"},{"key":"632_CR100","doi-asserted-by":"crossref","first-page":"12584","DOI":"10.1038\/s41598-017-12936-w","volume":"7","author":"L Kleppe","year":"2017","unstructured":"Kleppe, L. et al. Sex steroid production associated with puberty is absent in germ cell-free salmon. Sci. Rep. 7, 12584 (2017).","journal-title":"Sci. Rep."},{"key":"632_CR101","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.ydbio.2008.09.025","volume":"324","author":"KR Siegfried","year":"2008","unstructured":"Siegfried, K. R. & N\u00fcsslein-Volhard, C. Germ line control of female sex determination in zebrafish. Dev. Biol. 324, 277\u2013287 (2008).","journal-title":"Dev. Biol."},{"key":"632_CR102","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s11357-018-0049-4","volume":"41","author":"TL Habermehl","year":"2019","unstructured":"Habermehl, T. L. et al. Extension of longevity and reduction of inflammation is ovarian-dependent, but germ cell-independent in post-reproductive female mice. Geroscience 41, 25\u201338 (2019).","journal-title":"Geroscience"},{"key":"632_CR103","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1093\/gerona\/glp134","volume":"64","author":"JB Mason","year":"2009","unstructured":"Mason, J. B., Cargill, S. L., Anderson, G. B. & Carey, J. R. Transplantation of young ovaries to old mice increased life span in transplant recipients. J. Gerontol. A Biol. Sci. Med. Sci. 64, 1207\u20131211 (2009).","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"632_CR104","doi-asserted-by":"crossref","first-page":"3843","DOI":"10.1210\/en.2004-1616","volume":"146","author":"CD Toran-Allerand","year":"2005","unstructured":"Toran-Allerand, C. D., Tinnikov, A. A., Singh, R. J. & Nethrapalli, I. S. 17\u03b1-Estradiol: a brain-active estrogen? Endocrinology 146, 3843\u20133850 (2005).","journal-title":"Endocrinology"},{"key":"632_CR105","doi-asserted-by":"publisher","unstructured":"Lind, M. I. et al. Sex-specific growth and lifespan effects of germline removal in the dioecious nematode Caenorhabditis remanei. Preprint at bioRxiv https:\/\/doi.org\/10.1101\/2023.12.07.570570 (2023).","DOI":"10.1101\/2023.12.07.570570"},{"key":"632_CR106","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1098\/rspb.2005.3388","volume":"273","author":"AI Barnes","year":"2006","unstructured":"Barnes, A. I., Boone, J. M., Jacobson, J., Partridge, L. & Chapman, T. No extension of lifespan by ablation of germ line in Drosophila. Proc. R. Soc. B Biol. Sci. 273, 939\u2013947 (2006).","journal-title":"Proc. R. Soc. B Biol. Sci."},{"key":"632_CR107","doi-asserted-by":"crossref","first-page":"6368","DOI":"10.1073\/pnas.0709128105","volume":"105","author":"T Flatt","year":"2008","unstructured":"Flatt, T. et al. Drosophila germ-line modulation of insulin signaling and lifespan. Proc. Natl Acad. Sci. USA 105, 6368\u20136373 (2008).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"632_CR108","doi-asserted-by":"crossref","first-page":"15458","DOI":"10.1038\/ncomms15458","volume":"8","author":"Y Zhao","year":"2017","unstructured":"Zhao, Y. et al. Two forms of death in ageing Caenorhabditis elegans. Nat. Commun. 8, 15458 (2017).","journal-title":"Nat. Commun."},{"key":"632_CR109","doi-asserted-by":"crossref","first-page":"W171","DOI":"10.1093\/nar\/gkz365","volume":"47","author":"K Labun","year":"2019","unstructured":"Labun, K. et al. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Res. 47, W171\u2013W174 (2019).","journal-title":"Nucleic Acids Res."},{"key":"632_CR110","doi-asserted-by":"crossref","first-page":"13904","DOI":"10.1073\/pnas.1308335110","volume":"110","author":"LE Jao","year":"2013","unstructured":"Jao, L. E., Wente, S. R. & Chen, W. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc. Natl Acad. Sci. USA 110, 13904\u201313909 (2013).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"632_CR111","unstructured":"Astre, G., Moses, E. & Harel, I. Laboratory fish in biomedical research. In Laboratory Fish in Biomedical Research (eds D\u2019Angelo, L. & de Girolamo, P.) (Academic Press, 2021)."},{"key":"632_CR112","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/nprot.2013.046","volume":"8","author":"L Mazutis","year":"2013","unstructured":"Mazutis, L. et al. Single-cell analysis and sorting using droplet-based microfluidics. Nat. Protoc. 8, 870\u2013891 (2013).","journal-title":"Nat. Protoc."},{"key":"632_CR113","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1016\/j.devcel.2021.04.026","volume":"56","author":"D Bavli","year":"2021","unstructured":"Bavli, D. et al. CloneSeq: a highly sensitive analysis platform for the characterization of 3D-cultured single-cell-derived clones. Dev. Cell 56, 1804\u20131817.e7 (2021).","journal-title":"Dev. Cell"},{"key":"632_CR114","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","volume":"12","author":"B Li","year":"2011","unstructured":"Li, B. & Dewey, C. N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics 12, 323 (2011).","journal-title":"BMC Bioinformatics"},{"key":"632_CR115","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","volume":"9","author":"B Langmead","year":"2012","unstructured":"Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357\u2013359 (2012).","journal-title":"Nat. Methods"},{"key":"632_CR116","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-017-1381-1","volume":"19","author":"FA Wolf","year":"2018","unstructured":"Wolf, F. A., Angerer, P. & Theis, F. J. SCANPY: large-scale single-cell gene expression data analysis. Genome Biol. 19, 15 (2018).","journal-title":"Genome Biol."},{"key":"632_CR117","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nmeth.3971","volume":"13","author":"L Haghverdi","year":"2016","unstructured":"Haghverdi, L., B\u00fcttner, M., Wolf, F. A., Buettner, F. & Theis, F. J. Diffusion pseudotime robustly reconstructs lineage branching. Nat. Methods 13, 845\u2013848 (2016).","journal-title":"Nat. Methods"},{"key":"632_CR118","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","volume":"184","author":"Y Hao","year":"2021","unstructured":"Hao, Y. et al. Integrated analysis of multimodal single-cell data. Cell 184, 3573\u20133587 (2021).","journal-title":"Cell"},{"key":"632_CR119","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","volume":"16","author":"G Yu","year":"2012","unstructured":"Yu, G., Wang, L.-G., Han, Y. & He, Q.-Y. clusterProfiler: an R package for comparing biological themes among gene clusters. OMICS 16, 284\u2013287 (2012).","journal-title":"OMICS"},{"key":"632_CR120","doi-asserted-by":"publisher","unstructured":"Carlson, M., Falcon, S., Pages, H. & Li, N. org.Hs.eg.db: genome wide annotation for human. R package version 3.8.2. https:\/\/doi.org\/10.18129\/B9.bioc.org.Hs.eg.db (2019).","DOI":"10.18129\/B9.bioc.org.Hs.eg.db"},{"key":"632_CR121","unstructured":"Pag\u00e8s, H., Carlson, M., Falcon, S. & Li, N. AnnotationDbi: manipulation of SQLite-based annotations in Bioconductor. R package version1. 54.1. https:\/\/bioconductor.org\/packages\/devel\/bioc\/manuals\/AnnotationDbi\/man\/AnnotationDbi.pdf (2021)."},{"key":"632_CR122","unstructured":"Andrews, S. FastQC: a quality control tool for high throughput sequence data. https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/"},{"key":"632_CR123","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","volume":"32","author":"P Ewels","year":"2016","unstructured":"Ewels, P., Magnusson, M., Lundin, S. & K\u00e4ller, M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics 32, 3047\u20133048 (2016).","journal-title":"Bioinformatics"},{"key":"632_CR124","unstructured":"Krueger, F. Trim Galore. https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/trim_galore\/"},{"key":"632_CR125","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","volume":"17","author":"M Martin","year":"2011","unstructured":"Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 17, 10\u201312 (2011).","journal-title":"EMBnet J."},{"key":"632_CR126","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","volume":"29","author":"A Dobin","year":"2013","unstructured":"Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29, 15\u201321 (2013).","journal-title":"Bioinformatics"},{"key":"632_CR127","doi-asserted-by":"crossref","first-page":"4288","DOI":"10.1093\/nar\/gks042","volume":"40","author":"DJ McCarthy","year":"2012","unstructured":"McCarthy, D. J., Chen, Y. & Smyth, G. K. Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation. Nucleic Acids Res. 40, 4288\u20134297 (2012).","journal-title":"Nucleic Acids Res."},{"key":"632_CR128","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","volume":"26","author":"MD Robinson","year":"2010","unstructured":"Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26, 139\u2013140 (2010).","journal-title":"Bioinformatics"},{"key":"632_CR129","unstructured":"Leek, J. T. Surrogate variable analysis (PhD thesis, Univ. of Washington, 2007)."},{"key":"632_CR130","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1093\/bioinformatics\/btw313","volume":"32","author":"Z Gu","year":"2016","unstructured":"Gu, Z., Eils, R. & Schlesner, M. Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics 32, 2847\u20132849 (2016).","journal-title":"Bioinformatics"},{"key":"632_CR131","doi-asserted-by":"publisher","unstructured":"Carlson, M., Falcon, S., Pages, H. & Li, N. org.Mm.eg.db: genome wide annotation for mouse. R package version 3.8.2. https:\/\/doi.org\/10.18129\/B9.bioc.org.Mm.eg.db (2019).","DOI":"10.18129\/B9.bioc.org.Mm.eg.db"},{"key":"632_CR132","doi-asserted-by":"crossref","unstructured":"Dodzian, J., Kean, S., Seidel, J. & Valenzano, D. R. A protocol for laboratory housing of turquoise killifish (Nothobranchius furzeri). J. Vis. Exp. 57073 (2018).","DOI":"10.3791\/57073-v"},{"key":"632_CR133","doi-asserted-by":"crossref","first-page":"110350","DOI":"10.1016\/j.celrep.2022.110350","volume":"38","author":"H Boocholez","year":"2022","unstructured":"Boocholez, H. et al. Neuropeptide signaling and SKN-1 orchestrate differential responses of the proteostasis network to dissimilar proteotoxic insults. Cell Rep. 38, 110350 (2022).","journal-title":"Cell Rep."},{"key":"632_CR134","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1101\/gr.2505604","volume":"14","author":"J-F Rual","year":"2004","unstructured":"Rual, J.-F. et al. Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 14, 2162\u20132168 (2004).","journal-title":"Genome Res."},{"key":"632_CR135","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/nph.13852","volume":"210","author":"S Malitsky","year":"2016","unstructured":"Malitsky, S. et al. Viral infection of the marine alga Emiliania huxleyi triggers lipidome remodeling and induces the production of highly saturated triacylglycerol. New Phytol. 210, 88\u201396 (2016).","journal-title":"New Phytol."},{"key":"632_CR136","doi-asserted-by":"crossref","unstructured":"Salem, M., Bernach, M., Bajdzienko, K. & Giavalisco, P. A simple fractionated extraction method for the comprehensive analysis of metabolites, lipids, and proteins from a single sample. J. Vis. Exp. 55802 (2017).","DOI":"10.3791\/55802"},{"key":"632_CR137","doi-asserted-by":"crossref","first-page":"6001","DOI":"10.1038\/ncomms7001","volume":"6","author":"L Zheng","year":"2015","unstructured":"Zheng, L. et al. Fumarate induces redox-dependent senescence by modifying glutathione metabolism. Nat. Commun. 6, 6001 (2015).","journal-title":"Nat. Commun."},{"key":"632_CR138","doi-asserted-by":"crossref","unstructured":"Harel, I. & Brunet, A. The African turquoise killifish: a model for exploring vertebrate aging and diseases in the fast lane. Cold Spring Harb. Symp. Quant. Biol. 80, 275\u2013279 (2015).","DOI":"10.1101\/sqb.2015.80.027524"},{"key":"632_CR139","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1083\/jcb.200907080","volume":"187","author":"A Van Keymeulen","year":"2009","unstructured":"Van Keymeulen, A. et al. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. J. Cell Biol. 187, 91\u2013100 (2009).","journal-title":"J. Cell Biol."},{"key":"632_CR140","doi-asserted-by":"publisher","unstructured":"Harel, I. et al. Identification of protein aggregates in the aging vertebrate brain with prion-like and phase separation properties. Preprint at bioRxiv https:\/\/doi.org\/10.1101\/2022.02.26.482115 (2022).","DOI":"10.1101\/2022.02.26.482115"},{"key":"632_CR141","doi-asserted-by":"crossref","first-page":"11211","DOI":"10.1073\/pnas.1116065109","volume":"109","author":"Y Gruenbaum-Cohen","year":"2012","unstructured":"Gruenbaum-Cohen, Y. et al. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc. Natl Acad. Sci. USA 109, 11211\u201311216 (2012).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"632_CR142","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1242\/dev.049726","volume":"137","author":"S Theis","year":"2010","unstructured":"Theis, S. et al. The occipital lateral plate mesoderm is a novel source for vertebrate neck musculature. Development 137, 2961\u20132971 (2010).","journal-title":"Development"},{"key":"632_CR143","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1101\/gr.240093.118","volume":"29","author":"BA Benayoun","year":"2019","unstructured":"Benayoun, B. A. et al. Remodeling of epigenome and transcriptome landscapes with aging in mice reveals widespread induction of inflammatory responses. Genome Res. 29, 697\u2013709 (2019).","journal-title":"Genome Res."},{"key":"632_CR144","doi-asserted-by":"crossref","first-page":"18839","DOI":"10.1073\/pnas.1208690109","volume":"109","author":"I Harel","year":"2012","unstructured":"Harel, I. et al. Pharyngeal mesoderm regulatory network controls cardiac and head muscle morphogenesis. Proc. Natl Acad. Sci. USA 109, 18839\u201318844 (2012).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"632_CR145","unstructured":"Longenecker, K. & Langston, R. The Jungle Histology Atlas of Gonad Stages in Coral-Reef Fishes (Bishop Museum, 2016)."},{"key":"632_CR146","doi-asserted-by":"crossref","first-page":"3013","DOI":"10.1002\/dvdy.22443","volume":"239","author":"D Imrie","year":"2010","unstructured":"Imrie, D. & Sadler, K. C. White adipose tissue development in zebrafish is regulated by both developmental time and fish size. Dev. Dyn. 239, 3013\u20133023 (2010).","journal-title":"Dev. Dyn."},{"key":"632_CR147","first-page":"36","volume":"11","author":"MD Abr\u00e0moff","year":"2004","unstructured":"Abr\u00e0moff, M. D., Magalh\u00e3es, P. J. & Ram, S. J. Image processing with ImageJ. Biophotonics Intern. 11, 36\u201342 (2004).","journal-title":"Biophotonics Intern."},{"key":"632_CR148","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ygcen.2017.11.022","volume":"264","author":"L Hollander-Cohen","year":"2018","unstructured":"Hollander-Cohen, L., Golan, M., Aizen, J., Shpilman, M. & Levavi-Sivan, B. Characterization of carp gonadotropins: structure, annual profile, and carp and zebrafish pituitary topographic organization. Gen. Comp. Endocrinol. 264, 28\u201338 (2018).","journal-title":"Gen. Comp. Endocrinol."},{"key":"632_CR149","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1038\/s41598-017-02460-2","volume":"7","author":"Z Liu","year":"2017","unstructured":"Liu, Z. et al. Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Sci. Rep. 7, 2193 (2017).","journal-title":"Sci. Rep."},{"key":"632_CR150","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1111\/acel.12367","volume":"14","author":"S Wendler","year":"2015","unstructured":"Wendler, S., Hartmann, N., Hoppe, B. & Englert, C. Age-dependent decline in fin regenerative capacity in the short-lived fish Nothobranchius furzeri. Aging Cell 14, 857\u2013866 (2015).","journal-title":"Aging Cell"},{"key":"632_CR151","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1016\/j.cell.2014.11.021","volume":"159","author":"SSP Rao","year":"2014","unstructured":"Rao, S. S. P. et al. A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell 159, 1665\u20131680 (2014).","journal-title":"Cell"},{"key":"632_CR152","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.cels.2016.07.002","volume":"3","author":"NC Durand","year":"2016","unstructured":"Durand, N. C. et al. Juicer provides a one-click system for analyzing loop-resolution Hi-C experiments. Cell Syst. 3, 95\u201398 (2016).","journal-title":"Cell Syst."}],"container-title":["Nature Aging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s43587-024-00632-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s43587-024-00632-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s43587-024-00632-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T10:30:33Z","timestamp":1718793033000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s43587-024-00632-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,15]]},"references-count":152,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["632"],"URL":"https:\/\/doi.org\/10.1038\/s43587-024-00632-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.12.18.572041","asserted-by":"object"}]},"ISSN":["2662-8465"],"issn-type":[{"value":"2662-8465","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,15]]},"assertion":[{"value":"11 April 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}