{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:48:54Z","timestamp":1782323334589,"version":"3.54.5"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Med"],"DOI":"10.1038\/s43856-026-01713-4","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:06:36Z","timestamp":1782320796000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Capacity building for genomic surveillance of mpox and other emerging diseases in resource-limited settings within the African Great Lakes region"],"prefix":"10.1038","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1721-5879","authenticated-orcid":false,"given":"Leonard","family":"Schuele","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0971-2245","authenticated-orcid":false,"given":"N\u00e9h\u00e9mie","family":"Nzoyikorera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8370-7113","authenticated-orcid":false,"given":"Jean Claude","family":"Udahemuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley","family":"Cassidy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0688-5688","authenticated-orcid":false,"given":"Leandre","family":"Murhula Masirika","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1310-5031","authenticated-orcid":false,"given":"David F.","family":"Nieuwenhuijse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassien","family":"Nduwimana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9004-3850","authenticated-orcid":false,"given":"Richard","family":"Molenkamp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Nyandwi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Boter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jules","family":"Minega Ndoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7997-493X","authenticated-orcid":false,"given":"Luca M.","family":"Zaeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Pierre","family":"Musabyimana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2817-0127","authenticated-orcid":false,"given":"Rory D.","family":"de Vries","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin Bengehya","family":"Mbiribindi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freddy Belesi","family":"Siangoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saria","family":"Otani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7116-2723","authenticated-orcid":false,"given":"Frank M.","family":"Aarestrup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5204-2312","authenticated-orcid":false,"given":"Marion","family":"Koopmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pacifique","family":"Ndishimye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bas B.","family":"Oude Munnink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"1713_CR1","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1038\/nature16996","volume":"530","author":"J Quick","year":"2016","unstructured":"Quick, J. et al. Real-time, portable genome sequencing for Ebola surveillance. Nature 530, 228\u2013232 (2016). This study demonstrated the feasibility of mobile, on-site nanopore sequencing for genomic surveillance in LMIC settings.","journal-title":"Nature"},{"key":"1713_CR2","doi-asserted-by":"publisher","first-page":"1518","DOI":"10.1038\/s41591-021-01472-w","volume":"27","author":"BB Oude Munnink","year":"2021","unstructured":"Oude Munnink, B. B. et al. The next phase of SARS-CoV-2 surveillance: real-time molecular epidemiology. Nature medicine 27, 1518\u20131524 (2021).","journal-title":"Nature medicine"},{"key":"1713_CR3","doi-asserted-by":"publisher","first-page":"2480","DOI":"10.1038\/s41591-025-03720-9","volume":"31","author":"H Onywera","year":"2025","unstructured":"Onywera, H., Mulder, N., Kebede, Y. & Tessema, S. K. How to sustain a public-health genomics and bioinformatics workforce in Africa. Nature Medicine 31, 2480\u20132484 (2025).","journal-title":"Nature Medicine"},{"key":"1713_CR4","unstructured":"Africa CDC. Pathogen Genomic Surveillance Policy Framework. Addis Ababa: Africa CDC: 2025. Available from: https:\/\/africacdc.org\/download\/pathogen-genomic-surveillance-policy-framework\/ Policy framework for guidance of African Union Member States for the development of sustainable genomic surveillance strategies"},{"key":"1713_CR5","doi-asserted-by":"crossref","unstructured":"Goodfellow, I., Reusken, C. & Koopmans Dvm, M. Laboratory support during and after the Ebola virus endgame: towards a sustained laboratory infrastructure. Eurosurveillance 20 (2015).","DOI":"10.2807\/1560-7917.ES2015.20.12.21074"},{"key":"1713_CR6","doi-asserted-by":"publisher","unstructured":"Horizon Europe. Linking Infectious disease Front-liners\u2019 control Efforts with central public health authorities in The African Great Lakes Region. Brussels; 2023; Available from: https:\/\/doi.org\/10.3030\/101103059. Available from: https:\/\/www.globalsurveillance.eu\/projects\/great-life GREAT LIFE (Linking infectious disease front-liners\u2019 control efforts with central public health authorities in The African Great Lakes Region) framework with protocols, participating institutions and publications","DOI":"10.3030\/101103059"},{"key":"1713_CR7","doi-asserted-by":"publisher","unstructured":"Horizon Europe. Joint Understanding and Analysis of clade I monkeypox epidemiology, evolution and immunology in South Kivu. Brussels; 2024. Available from: https:\/\/doi.org\/10.3030\/101195116. https:\/\/juakivu.org","DOI":"10.3030\/101195116"},{"key":"1713_CR8","doi-asserted-by":"publisher","first-page":"2400106","DOI":"10.2807\/1560-7917.ES.2024.29.11.2400106","volume":"29","author":"LM Masirika","year":"2024","unstructured":"Masirika, L. M. et al. Ongoing mpox outbreak in Kamituga, South Kivu province, associated with monkeypox virus of a novel Clade I sub-lineage, Democratic Republic of the Congo, 2024. Eurosurveillance 29, 2400106 (2024).","journal-title":"Eurosurveillance"},{"key":"1713_CR9","doi-asserted-by":"publisher","first-page":"2065","DOI":"10.3201\/eid2910.230824","volume":"29","author":"CK Yinda","year":"2023","unstructured":"Yinda, C. K. et al. Stability of monkeypox virus in body fluids and wastewater. Emerging infectious diseases 29, 2065 (2023).","journal-title":"Emerging infectious diseases"},{"key":"1713_CR10","doi-asserted-by":"publisher","first-page":"3021","DOI":"10.1128\/JCM.00514-11","volume":"49","author":"A-L Page","year":"2011","unstructured":"Page, A.-L. et al. Use of filter paper as a transport medium for laboratory diagnosis of cholera under field conditions. Journal of clinical microbiology 49, 3021\u20133023 (2011).","journal-title":"Journal of clinical microbiology"},{"key":"1713_CR11","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","volume":"169","author":"Y Li","year":"2010","unstructured":"Li, Y., Zhao, H., Wilkins, K., Hughes, C. & Damon, I. K. Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA. Journal of virological methods 169, 223\u2013227 (2010).","journal-title":"Journal of virological methods"},{"key":"1713_CR12","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1016\/S0140-6736(24)01825-7","volume":"404","author":"RS Dhillon","year":"2024","unstructured":"Dhillon, R. S., Karan, A., Srikrishna, D. & Mombouli, J.-V. The need for point-of-care tests for mpox. The Lancet 404, 1193\u20131194 (2024).","journal-title":"The Lancet"},{"key":"1713_CR13","doi-asserted-by":"crossref","unstructured":"Pronyk, P. M. et al. Advancing pathogen genomics in resource-limited settings. Cell Genomics 3 (2023).","DOI":"10.1016\/j.xgen.2023.100443"},{"key":"1713_CR14","doi-asserted-by":"publisher","first-page":"2400486","DOI":"10.2807\/1560-7917.ES.2024.29.32.2400486","volume":"29","author":"L Schuele","year":"2024","unstructured":"Schuele, L. et al. Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024. Eurosurveillance 29, 2400486 (2024).","journal-title":"Eurosurveillance"},{"key":"1713_CR15","unstructured":"World Health Organization (22 May 2026). Disease Outbreak News; Bundibugyo Virus Disease, Democratic Republic of the Congo. Available at: https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2026-DON603"},{"key":"1713_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47518-8","volume":"15","author":"Y Wang","year":"2024","unstructured":"Wang, Y. et al. Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device. Nature communications 15, 3279 (2024).","journal-title":"Nature communications"},{"key":"1713_CR17","doi-asserted-by":"publisher","first-page":"343295","DOI":"10.1016\/j.aca.2024.343295","volume":"1336","author":"Y Chen","year":"2025","unstructured":"Chen, Y., Zhao, R., Hu, X. & Wang, X. The current status and future prospects of CRISPR-based detection of monkeypox virus: A review. Analytica Chimica Acta 1336, 343295 (2025).","journal-title":"Analytica Chimica Acta"},{"key":"1713_CR18","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1126\/science.aau9343","volume":"363","author":"LE Kafetzopoulou","year":"2019","unstructured":"Kafetzopoulou, L. E. et al. Metagenomic sequencing at the epicenter of the Nigeria 2018 Lassa fever outbreak. Science 363, 74\u201377 (2019).","journal-title":"Science"},{"key":"1713_CR19","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1038\/nprot.2017.066","volume":"12","author":"J Quick","year":"2017","unstructured":"Quick, J. et al. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. Nature protocols 12, 1261\u20131276 (2017).","journal-title":"Nature protocols"},{"key":"1713_CR20","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1038\/s43856-025-01199-6","volume":"5","author":"N Nzoyikorera","year":"2025","unstructured":"Nzoyikorera, N. et al. Genomic monitoring and tracking of mpox virus clade Ib in Burundi between july to september 2024. Communications Medicine 5, 494 (2025).","journal-title":"Communications Medicine"},{"key":"1713_CR21","unstructured":"Kovalenko, G. A., Hosmillo, M., Quick, J., Goodfellow, I. & Kent, C. ARTIC HELP protocol for amplicon-based viral genome sequencing. (2025)."},{"key":"1713_CR22","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3390\/tropicalmed10040090","volume":"10","author":"E Agboli","year":"2025","unstructured":"Agboli, E. et al. Building pathogen genomic sequencing capacity in Africa: Centre for epidemic response and innovation fellowship. Tropical Medicine and Infectious Disease 10, 90 (2025).","journal-title":"Tropical Medicine and Infectious Disease"}],"container-title":["Communications Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s43856-026-01713-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s43856-026-01713-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s43856-026-01713-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:06:45Z","timestamp":1782320805000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s43856-026-01713-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,24]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["1713"],"URL":"https:\/\/doi.org\/10.1038\/s43856-026-01713-4","relation":{},"ISSN":["2730-664X"],"issn-type":[{"value":"2730-664X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,24]]},"assertion":[{"value":"25 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing of interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"356"}}