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Samples were collected from pig pen environments and analysed using shotgun metagenomic sequencing. Resistome and microbiome profiles showed stronger temporal than farm-specific variation, with several age-associated trends. Age-related trajectories diverged between microbiome and resistome, indicating that resistance dynamics are shaped by factors beyond microbial succession. The highest relative abundance of resistance determinants was observed for tetracyclines, followed by aminoglycosides, macrolide\u2013lincosamide\u2013streptogramin antibiotics, beta-lactams, and folic acid synthesis inhibitors\u2014drug classes commonly used in Swedish pig production. Resistome patterns were partially associated with phenotypic resistance profiles from previous studies, while analysis of antimicrobial usage alone could not fully explain the observed resistome. Overall, these findings suggest that additional factors beyond antimicrobial usage contribute to the persistence and dissemination of antibiotic resistance genes in pig farm environments.<\/jats:p>","DOI":"10.1038\/s44259-026-00251-2","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:24:46Z","timestamp":1784766286000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Longitudinal investigation of the resistomes in Swedish pig farms"],"prefix":"10.1038","volume":"4","author":[{"given":"Valeriia","family":"Ladyhina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Sternberg-Lewerin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Sann\u00f6","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Bongcam-Rudloff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Dicksved","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Rajala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"251_CR1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","volume":"399","author":"C Murray","year":"2022","unstructured":"Murray, C. et al. 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