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Co-funded by Plan Operativo de Empleo Juvenil"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO J"],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Meiosis is the developmental program that generates gametes. To produce healthy gametes, meiotic recombination creates reciprocal exchanges between each pair of homologous chromosomes that facilitate faithful chromosome segregation. Using fission yeast and biochemical, genetic, and cytological approaches, we have studied the role of CDK (cyclin-dependent kinase) in the control of Swi5\u2013Sfr1, a Rad51-recombinase auxiliary factor involved in homolog invasion during recombination. We show that Sfr1 is a CDK target, and its phosphorylation downregulates Swi5\u2013Sfr1 function in the meiotic prophase. Expression of a phospho-mimetic\n                    <jats:italic>sfr1-7D<\/jats:italic>\n                    mutant inhibits Rad51 binding, its robust chromosome loading, and subsequently decreases interhomolog recombination. On the other hand, the non-phosphorylatable\n                    <jats:italic>sfr1-7A<\/jats:italic>\n                    mutant alters Rad51 dynamics at late prophase, and exacerbates chromatin segregation defects and Rad51 retention observed in\n                    <jats:italic>dbl2<\/jats:italic>\n                    deletion mutants when combined with them. We propose Sfr1 phospho-inhibition as a novel cell-cycle-dependent mechanism, which ensures timely resolution of recombination intermediates and successful chromosome distribution into the gametes. Furthermore, the N-terminal disordered part of Sfr1, an evolutionarily conserved feature, serves as a regulatory platform coordinating this phospho-regulation, protein localization and stability, with several CDK sites and regulatory sequences being conserved.\n                  <\/jats:p>","DOI":"10.1038\/s44318-024-00205-2","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T09:40:27Z","timestamp":1724406027000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["CDK phosphorylation of Sfr1 downregulates Rad51 function in late-meiotic homolog invasions"],"prefix":"10.1038","volume":"43","author":[{"given":"In\u00e9s","family":"Palacios-Blanco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Bort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9092-5175","authenticated-orcid":false,"given":"Nina","family":"Mayerov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7461-9071","authenticated-orcid":false,"given":"Silvia","family":"B\u00e1ge\u013eov\u00e1 Pol\u00e1kov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1303-2517","authenticated-orcid":false,"given":"Cristina","family":"Mart\u00edn-Castellanos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"205_CR1","doi-asserted-by":"publisher","first-page":"e1001160","DOI":"10.1371\/journal.pgen.1001160","volume":"6","author":"Y Akamatsu","year":"2010","unstructured":"Akamatsu Y, Jasin M (2010) Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination. 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