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While mutations in the kinesin-like motor protein\n                    <jats:italic>KIF23<\/jats:italic>\n                    gene have been recently linked to microcephaly in humans, the underlying mechanisms remain elusive. Here, we explore the pivotal role of KIF23 in embryonic cortical development. We characterize the dynamic expression of KIF23 in the cortical NSPCs of mice, ferrets, and humans during embryonic neurogenesis. Knockdown of\n                    <jats:italic>Kif23<\/jats:italic>\n                    in mice results in precocious neurogenesis and neuronal apoptosis, attributed to an accelerated cell cycle exit, likely resulting from disrupted mitotic spindle orientation and impaired cytokinesis. Additionally, KIF23 depletion perturbs the apical surface structure of NSPCs by affecting the localization of apical junction proteins. We further demonstrate that the phenotypes induced by\n                    <jats:italic>Kif23<\/jats:italic>\n                    knockdown are rescued by introducing wild-type human\n                    <jats:italic>KIF23<\/jats:italic>\n                    , but not by a microcephaly-associated variant. Our findings unveil a previously unexplored role of KIF23 in neural stem and progenitor cell maintenance via regulating spindle orientation and apical structure in addition to cytokinesis, shedding light on microcephaly pathogenesis.\n                  <\/jats:p>","DOI":"10.1038\/s44318-024-00327-7","type":"journal-article","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T05:02:24Z","timestamp":1733288544000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Kinesin-like motor protein KIF23 maintains neural stem\u00a0and progenitor cell pools in the developing cortex"],"prefix":"10.1038","volume":"44","author":[{"given":"Sharmin","family":"Naher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0829-5299","authenticated-orcid":false,"given":"Kenji","family":"Iemura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Miyashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9526-3473","authenticated-orcid":false,"given":"Mikio","family":"Hoshino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6086-2858","authenticated-orcid":false,"given":"Kozo","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8367-9228","authenticated-orcid":false,"given":"Shinsuke","family":"Niwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0135-759X","authenticated-orcid":false,"given":"Jin-Wu","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6643-8289","authenticated-orcid":false,"given":"Takako","family":"Kikkawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0548-6452","authenticated-orcid":false,"given":"Noriko","family":"Osumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,4]]},"reference":[{"key":"327_CR1","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1101\/gad.12.10.1483","volume":"12","author":"RR Adams","year":"1998","unstructured":"Adams RR, Tavares AA, Salzberg A, Bellen HJ, Glover DM (1998) pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis. 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