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Electrophysiological recordings at sub-200 \u00b5s resolution show a transient increase in host cell membrane conductance following parasite exposure. Transients always precede invasion, but parasites depleted of RON2 generate transients without invading.\u00a0Thus RON2 is not essential for transient generation. Time-series analysis developed here and applied to the 910,000 data point transient dataset reveal multiple quantal conductance changes in the parasite-induced transient, consistent with rapid insertion, then slower removal, blocking, or inactivation of potential pore components. Quantal steps for wild-type RH strain parasites have a principal mode with Gaussian mean of 0.26\u2009nS, similar in step size to the pore forming protein EXP2, part of the PTEX translocon of malaria parasites. Without RON2 the quantal mean (0.19\u2009nS) is significantly different. Because we observe no parasite invasion without poration, the term \u201cinvasion pore\u201d is proposed to describe this transient breach in host cell membrane barrier integrity during invasion.\n                  <\/jats:p>","DOI":"10.1038\/s44319-025-00565-8","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T09:03:13Z","timestamp":1758272593000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The invasion pore induced by Toxoplasma gondii"],"prefix":"10.1038","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7497-096X","authenticated-orcid":false,"given":"Yuto","family":"Kegawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances","family":"Male","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1588-9511","authenticated-orcid":false,"given":"Irene","family":"Jim\u00e9nez-Mungu\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6285-4277","authenticated-orcid":false,"given":"Paul S","family":"Blank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Mekhedov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4138-3055","authenticated-orcid":false,"given":"Gary E","family":"Ward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1707-3789","authenticated-orcid":false,"given":"Joshua","family":"Zimmerberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"565_CR1","first-page":"0137","volume":"2","author":"DL Alexander","year":"2005","unstructured":"Alexander DL, Mital J, Ward GE, Bradley P, Boothroyd JC (2005) Identification of the moving junction complesx of Toxoplasma gondii: a collaboration between distinct secretory organelles. 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