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Here, we identify the R2R3-MYB transcription factor MYB4 as a previously unknown negative regulator of Fe acquisition. MYB4 directly interacts with FIT, competing with bHLH38\/39 to prevent the formation of transcriptionally active heterodimers, thereby repressing the expression of the key Fe-uptake genes\n                    <jats:italic>IRT1<\/jats:italic>\n                    and\n                    <jats:italic>FRO2<\/jats:italic>\n                    . We establish that the mitogen-activated protein kinase MPK3 acts upstream in this pathway. Under Fe-deficient conditions, MPK3 is activated and directly phosphorylates MYB4 at Serine 4, promoting its proteasome-dependent destabilization. This post-translational switch releases FIT to dimerize with bHLH partners, activating Fe uptake. Our work unveils the MPK3-MYB4-FIT signaling module as a previously unrecognized regulatory module that contributes to the dynamic control of Fe homeostasis in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s44319-026-00906-1","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:32:15Z","timestamp":1787661135000},"page":"5632-5658","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MPK3-mediated phosphorylation inactivates MYB4 to promote FIT-dependent iron acquisition"],"prefix":"10.1038","volume":"27","author":[{"given":"Hui","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0415-6208","authenticated-orcid":false,"given":"Guangna","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6301-8361","authenticated-orcid":false,"given":"Jiena","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8632-7972","authenticated-orcid":false,"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4937-9664","authenticated-orcid":false,"given":"Shuangyao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9977-2130","authenticated-orcid":false,"given":"Shuxun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6008-1259","authenticated-orcid":false,"given":"Yanjun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8530-4737","authenticated-orcid":false,"given":"Li","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0277-9754","authenticated-orcid":false,"given":"Xi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6693-877X","authenticated-orcid":false,"given":"Shuqing","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"906_CR1","doi-asserted-by":"publisher","first-page":"1699","DOI":"10.1080\/01904169209364432","volume":"15","author":"J Abad\u00eda","year":"1992","unstructured":"Abad\u00eda J (1992) Leaf responses to Fe deficiency: a review. 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