{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:49:52Z","timestamp":1789487392838,"version":"build-2803163510"},"reference-count":66,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["CRG\/2022\/005151"],"award-info":[{"award-number":["CRG\/2022\/005151"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["ANRF\/ARG\/2025\/003980\/LS"],"award-info":[{"award-number":["ANRF\/ARG\/2025\/003980\/LS"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001852","name":"Indo-French Centre for the Promotion of Advanced Research","doi-asserted-by":"publisher","award":["7103-4"],"award-info":[{"award-number":["7103-4"]}],"id":[{"id":"10.13039\/501100001852","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institute of Eminence funds","award":["IE\/REDA-23-2061,IE\/REDA-24-2061,IE\/REDA-25-2061"],"award-info":[{"award-number":["IE\/REDA-23-2061,IE\/REDA-24-2061,IE\/REDA-25-2061"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Animal cells assemble gigadalton-scale molecular complexes composed of ~200 proteins to build a functional centrosome during mitosis, ensuring faithful chromosome segregation. Although the molecular principles underlying centrosome assembly have been extensively studied, the minimal components required to reconstitute centrosome-like activity in living cells remain unknown. Here, we develop an optogenetic strategy that exploits Aurora A kinase clustering to assemble functional centrosome-like structures in human cells. Remarkably, these light-induced assemblies activate Aurora A, nucleate microtubules, and bypass the requirement for the canonical Aurora A scaffold Cep192. Mechanistically, we identify the evolutionarily conserved Aurora A partner TPX2 as an essential factor for Aurora A cluster-dependent microtubule nucleation. Furthermore, optogenetic Aurora A clustering significantly rescues spindle assembly defects associated with centrosome ablation. We term this approach Light-Induced Spindle Assembly (LISA). By directly coupling Aurora A activity to spatial clustering, LISA defines a minimal and tunable module capable of driving spindle assembly. More broadly, LISA provides a synthetic biology platform for dissecting the fundamental principles of Aurora A-dependent spindle assembly, a prominent feature of mitotic and meiotic spindle formation.<\/jats:p>","DOI":"10.1038\/s44319-026-00928-9","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:53:05Z","timestamp":1789476785000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Light-induced spindle assembly through Aurora A clustering"],"prefix":"10.1038","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2449-7176","authenticated-orcid":false,"given":"Vignesh","family":"Olakkal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3646-2015","authenticated-orcid":false,"given":"Madhumitha","family":"Balakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0219-7970","authenticated-orcid":false,"given":"Sachin","family":"Kotak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"928_CR1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/S1535-6108(02)00235-0","volume":"3","author":"S Anand","year":"2003","unstructured":"Anand S, Penrhyn-Lowe S, Venkitaraman AR (2003) AURORA-A amplification overrides the mitotic spindle assembly checkpoint, inducing resistance to Taxol. Cancer Cell 3:51\u201362","journal-title":"Cancer Cell"},{"key":"928_CR2","doi-asserted-by":"publisher","first-page":"2987","DOI":"10.1242\/jcs.013136","volume":"120","author":"AR Barr","year":"2007","unstructured":"Barr AR, Gergely F (2007) Aurora-A: the maker and breaker of spindle poles. J Cell Sci 120:2987\u20132996","journal-title":"J Cell Sci"},{"key":"928_CR3","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1016\/S1097-2765(03)00392-7","volume":"12","author":"R Bayliss","year":"2003","unstructured":"Bayliss R, Sardon T, Vernos I, Conti E (2003) Structural basis of Aurora-A activation by TPX2 at the mitotic spindle. Mol Cell 12:851\u2013862","journal-title":"Mol Cell"},{"key":"928_CR4","doi-asserted-by":"publisher","first-page":"e63230","DOI":"10.7554\/eLife.63230","volume":"9","author":"L Benedetti","year":"2020","unstructured":"Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, De Camilli P (2020) Optimized vivid-derived magnets photodimerizers for subcellular optogenetics in mammalian cells. Elife 9:e63230","journal-title":"Elife"},{"key":"928_CR5","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1083\/jcb.200802005","volume":"182","author":"AW Bird","year":"2008","unstructured":"Bird AW, Hyman AA (2008) Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. J Cell Biol 182:289\u2013300","journal-title":"J Cell Biol"},{"key":"928_CR6","doi-asserted-by":"publisher","first-page":"5318","DOI":"10.1091\/mbc.e04-05-0385","volume":"15","author":"S Brunet","year":"2004","unstructured":"Brunet S, Sardon T, Zimmerman T, Wittmann T, Pepperkok R, Karsenti E, Vernos I (2004) Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts. Mol Biol Cell 15:5318\u20135328","journal-title":"Mol Biol Cell"},{"key":"928_CR7","doi-asserted-by":"publisher","first-page":"160089","DOI":"10.1098\/rsob.160089","volume":"6","author":"SG Burgess","year":"2016","unstructured":"Burgess SG, Oleksy A, Cavazza T, Richards MW, Vernos I, Matthews D, Bayliss R (2016) Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domain. Open Biol 6:160089","journal-title":"Open Biol"},{"key":"928_CR8","doi-asserted-by":"publisher","first-page":"7506","DOI":"10.1074\/jbc.M112.439935","volume":"288","author":"Y Chen","year":"2013","unstructured":"Chen Y, Sawada O, Kohno H, Le YZ, Subauste C, Maeda T, Maeda A (2013) Autophagy protects the retina from light-induced degeneration. J Biol Chem 288:7506\u20137518","journal-title":"J Biol Chem"},{"key":"928_CR9","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019102378","volume":"39","author":"T Chinen","year":"2020","unstructured":"Chinen T, Yamamoto S, Takeda Y, Watanabe K, Kuroki K, Hashimoto K, Takao D, Kitagawa D (2020) NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells. EMBO J 39:e102378","journal-title":"EMBO J"},{"key":"928_CR10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202006085","volume":"220","author":"T Chinen","year":"2021","unstructured":"Chinen T, Yamazaki K, Hashimoto K, Fujii K, Watanabe K, Takeda Y, Yamamoto S, Nozaki Y, Tsuchiya Y, Takao D et al (2021) Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly. J Cell Biol 220:e202006085","journal-title":"J Cell Biol"},{"key":"928_CR11","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1016\/j.devcel.2014.02.013","volume":"28","author":"PT Conduit","year":"2014","unstructured":"Conduit PT, Feng Z, Richens JH, Baumbach J, Wainman A, Bakshi SD, Dobbelaere J, Johnson S, Lea SM, Raff JW (2014) The centrosome-specific phosphorylation of Cnn by Polo\/Plk1 drives Cnn scaffold assembly and centrosome maturation. Dev Cell 28:659\u2013669","journal-title":"Dev Cell"},{"key":"928_CR12","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1038\/nrm4062","volume":"16","author":"PT Conduit","year":"2015","unstructured":"Conduit PT, Wainman A, Raff JW (2015) Centrosome function and assembly in animal cells. Nat Rev Mol Cell Biol 16:611\u2013624","journal-title":"Nat Rev Mol Cell Biol"},{"key":"928_CR13","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.1074\/jbc.M111.312090","volume":"287","author":"CA Dodson","year":"2012","unstructured":"Dodson CA, Bayliss R (2012) Activation of Aurora-A kinase by protein partner binding and phosphorylation are independent and synergistic. J Biol Chem 287:1150\u20131157","journal-title":"J Biol Chem"},{"key":"928_CR14","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1016\/j.chembiol.2015.04.014","volume":"22","author":"L Duan","year":"2015","unstructured":"Duan L, Che D, Zhang K, Ong Q, Guo S, Cui B (2015) Optogenetic control of molecular motors and organelle distributions in cells. Chem Biol 22:671\u2013682","journal-title":"Chem Biol"},{"key":"928_CR15","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1016\/S0960-9822(03)00166-0","volume":"13","author":"PA Eyers","year":"2003","unstructured":"Eyers PA, Erikson E, Chen LG, Maller JL (2003) A novel mechanism for activation of the protein kinase Aurora A. Curr Biol 13:691\u2013697","journal-title":"Curr Biol"},{"key":"928_CR16","doi-asserted-by":"publisher","first-page":"1960","DOI":"10.1016\/j.cub.2007.10.019","volume":"17","author":"MA Gomez-Ferreria","year":"2007","unstructured":"Gomez-Ferreria MA, Rath U, Buster DW, Chanda SK, Caldwell JS, Rines DR, Sharp DJ (2007) Human Cep192 is required for mitotic centrosome and spindle assembly. Curr Biol 17:1960\u20131966","journal-title":"Curr Biol"},{"key":"928_CR17","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1126\/science.aay9959","volume":"367","author":"A Hadzipasic","year":"2020","unstructured":"Hadzipasic A, Wilson C, Nguyen V, Kern N, Kim C, Pitsawong W, Villali J, Zheng Y, Kern D (2020) Ancient origins of allosteric activation in a Ser-Thr kinase. Science 367:912\u2013917","journal-title":"Science"},{"key":"928_CR18","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/S1534-5807(02)00327-1","volume":"3","author":"DR Hamill","year":"2002","unstructured":"Hamill DR, Severson AF, Carter JC, Bowerman B (2002) Centrosome maturation and mitotic spindle assembly in C. elegans require SPD-5, a protein with multiple coiled-coil domains. Dev Cell 3:673\u2013684","journal-title":"Dev Cell"},{"key":"928_CR19","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1083\/jcb.200108051","volume":"155","author":"E Hannak","year":"2001","unstructured":"Hannak E, Kirkham M, Hyman AA, Oegema K (2001) Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. J Cell Biol 155:1109\u20131116","journal-title":"J Cell Biol"},{"key":"928_CR20","doi-asserted-by":"publisher","first-page":"e5976","DOI":"10.1371\/journal.pone.0005976","volume":"4","author":"L Haren","year":"2009","unstructured":"Haren L, Stearns T, Luders J (2009) Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components. PLoS ONE 4:e5976","journal-title":"PLoS ONE"},{"key":"928_CR21","doi-asserted-by":"publisher","first-page":"5381","DOI":"10.1038\/s44318-024-00240-z","volume":"43","author":"J Holder","year":"2024","unstructured":"Holder J, Miles JA, Batchelor M, Popple H, Walko M, Yeung W, Kannan N, Wilson AJ, Bayliss R, Gergely F (2024) CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2. EMBO J 43:5381\u20135420","journal-title":"EMBO J"},{"key":"928_CR22","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aar4195","volume":"11","author":"V Joukov","year":"2018","unstructured":"Joukov V, De Nicolo A (2018) Aurora-PLK1 cascades as key signaling modules in the regulation of mitosis. Sci Signal 11:eaar4195","journal-title":"Sci Signal"},{"key":"928_CR23","doi-asserted-by":"publisher","first-page":"21022","DOI":"10.1073\/pnas.1014664107","volume":"107","author":"V Joukov","year":"2010","unstructured":"Joukov V, De Nicolo A, Rodriguez A, Walter JC, Livingston DM (2010) Centrosomal protein of 192 kDa (Cep192) promotes centrosome-driven spindle assembly by engaging in organelle-specific Aurora A activation. Proc Natl Acad Sci USA 107:21022\u201321027","journal-title":"Proc Natl Acad Sci USA"},{"key":"928_CR24","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1016\/j.molcel.2014.06.016","volume":"55","author":"V Joukov","year":"2014","unstructured":"Joukov V, Walter JC, De Nicolo A (2014) The Cep192-organized Aurora A-Plk1 cascade is essential for centrosome cycle and bipolar spindle assembly. Mol Cell 55:578\u2013591","journal-title":"Mol Cell"},{"key":"928_CR25","doi-asserted-by":"crossref","unstructured":"Kapoor S, Kotak S (2019) Centrosome Aurora A regulates RhoGEF ECT-2 localisation and ensures a single PAR-2 polarity axis in C. elegans embryos. Development 146:dev174565","DOI":"10.1242\/dev.174565"},{"key":"928_CR26","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1083\/jcb.153.1.237","volume":"153","author":"A Khodjakov","year":"2001","unstructured":"Khodjakov A, Rieder CL (2001) Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. J Cell Biol 153:237\u2013242","journal-title":"J Cell Biol"},{"key":"928_CR27","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1083\/jcb.201209107","volume":"201","author":"E Kress","year":"2013","unstructured":"Kress E, Schwager F, Holtackers R, Seiler J, Prodon F, Zanin E, Eiteneuer A, Toya M, Sugimoto A, Meyer H et al (2013) The UBXN-2\/p37\/p47 adaptors of CDC-48\/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. J Cell Biol 201:559\u2013575","journal-title":"J Cell Biol"},{"key":"928_CR28","doi-asserted-by":"publisher","first-page":"R648","DOI":"10.1016\/j.cub.2015.05.060","volume":"25","author":"T Laos","year":"2015","unstructured":"Laos T, Cabral G, Dammermann A (2015) Isotropic incorporation of SPD-5 underlies centrosome assembly in C. elegans. Curr Biol 25:R648\u2013R649","journal-title":"Curr Biol"},{"key":"928_CR29","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1038\/nmeth.2940","volume":"11","author":"S Lee","year":"2014","unstructured":"Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD (2014) Reversible protein inactivation by optogenetic trapping in cells. Nat Methods 11:633\u2013636","journal-title":"Nat Methods"},{"key":"928_CR30","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202404025","volume":"224","author":"Z Liang","year":"2025","unstructured":"Liang Z, Huang J, Wang Y, Hua S, Jiang K (2025) Diverse microtubule-binding repeats regulate TPX2 activities at distinct locations within the spindle. J Cell Biol 224:e202404025","journal-title":"J Cell Biol"},{"key":"928_CR31","doi-asserted-by":"publisher","first-page":"302","DOI":"10.15252\/embj.201592929","volume":"35","author":"J Lipka","year":"2016","unstructured":"Lipka J, Kapitein LC, Jaworski J, Hoogenraad CC (2016) Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites. EMBO J 35:302\u2013318","journal-title":"EMBO J"},{"key":"928_CR32","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1126\/science.1163927","volume":"322","author":"H Liu","year":"2008","unstructured":"Liu H, Yu X, Li K, Klejnot J, Yang H, Lisiero D, Lin C (2008) Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis. Science 322:1535\u20131539","journal-title":"Science"},{"key":"928_CR34","doi-asserted-by":"publisher","DOI":"10.3390\/biom9010028","volume":"9","author":"L Magnaghi-Jaulin","year":"2019","unstructured":"Magnaghi-Jaulin L, Eot-Houllier G, Gallaud E, Giet R (2019) Aurora A protein kinase: to the centrosome and beyond. Biomolecules 9:biom9010028","journal-title":"Biomolecules"},{"key":"928_CR35","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aed5283","volume":"12","author":"JL Meaders","year":"2026","unstructured":"Meaders JL, Rodriguez AA, Variyar S, Park S, Cirulli AE, Oegema K, Corbett KD, Desai A (2026) A chromatin-associated pool of Aurora A controls kinetochore-microtubule attachments to ensure chromosome biorientation. Sci Adv 12:eaed5283","journal-title":"Sci Adv"},{"key":"928_CR36","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1083\/jcb.201604081","volume":"214","author":"F Meitinger","year":"2016","unstructured":"Meitinger F, Anzola JV, Kaulich M, Richardson A, Stender JD, Benner C, Glass CK, Dowdy SF, Desai A, Shiau AK et al (2016) 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol 214:155\u2013166","journal-title":"J Cell Biol"},{"key":"928_CR37","doi-asserted-by":"publisher","first-page":"3917","DOI":"10.1038\/s41388-021-01766-w","volume":"40","author":"FD Naso","year":"2021","unstructured":"Naso FD, Boi D, Ascanelli C, Pamfil G, Lindon C, Paiardini A, Guarguaglini G (2021) Nuclear localisation of Aurora-A: its regulation and significance for Aurora-A functions in cancer. Oncogene 40:3917\u20133928","journal-title":"Oncogene"},{"key":"928_CR38","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1038\/nchembio.2064","volume":"12","author":"MK Nguyen","year":"2016","unstructured":"Nguyen MK, Kim CY, Kim JM, Park BO, Lee S, Park H, Heo WD (2016) Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking. Nat Chem Biol 12:431\u2013436","journal-title":"Nat Chem Biol"},{"key":"928_CR40","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202009083","volume":"220","author":"M Ohta","year":"2021","unstructured":"Ohta M, Zhao Z, Wu D, Wang S, Harrison JL, Gomez-Cavazos JS, Desai A, Oegema KF (2021) Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome. J Cell Biol 220:e202009083","journal-title":"J Cell Biol"},{"key":"928_CR41","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/S0070-2153(99)49021-0","volume":"49","author":"RE Palazzo","year":"2000","unstructured":"Palazzo RE, Vogel JM, Schnackenberg BJ, Hull DR, Wu X (2000) Centrosome maturation. Curr Top Dev Biol 49:449\u2013470","journal-title":"Curr Top Dev Biol"},{"key":"928_CR42","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf8582","volume":"9","author":"JG Park","year":"2023","unstructured":"Park JG, Jeon H, Shin S, Song C, Lee H, Kim NK, Kim EE, Hwang KY, Lee BJ, Lee IG (2023) Structural basis for CEP192-mediated regulation of centrosomal AURKA. Sci Adv 9:eadf8582","journal-title":"Sci Adv"},{"key":"928_CR43","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.ceb.2021.02.002","volume":"71","author":"JB Passmore","year":"2021","unstructured":"Passmore JB, Nijenhuis W, Kapitein LC (2021) From observing to controlling: Inducible control of organelle dynamics and interactions. Curr Opin Cell Biol 71:69\u201376","journal-title":"Curr Opin Cell Biol"},{"key":"928_CR44","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1016\/j.cell.2012.12.044","volume":"152","author":"S Petry","year":"2013","unstructured":"Petry S, Groen AC, Ishihara K, Mitchison TJ, Vale RD (2013) Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2. Cell 152:768\u2013777","journal-title":"Cell"},{"key":"928_CR45","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1038\/35007077","volume":"404","author":"RD Phair","year":"2000","unstructured":"Phair RD, Misteli T (2000) High mobility of proteins in the mammalian cell nucleus. Nature 404:604\u2013609","journal-title":"Nature"},{"key":"928_CR46","doi-asserted-by":"publisher","first-page":"2741","DOI":"10.1038\/s44318-025-00421-4","volume":"44","author":"R Raja","year":"2025","unstructured":"Raja R, Biswas B, Abraham R, Wang Y, Chang CY, Hendriks IA, Buch-Larsen SC, Liu H, Yang X, Wang C et al (2025) Interferon-induced PARP14-mediated ADP-ribosylation in p62 bodies requires the ubiquitin-proteasome system. EMBO J 44:2741\u20132773","journal-title":"EMBO J"},{"key":"928_CR47","doi-asserted-by":"publisher","first-page":"6797","DOI":"10.1038\/s44318-025-00564-4","volume":"44","author":"A Rajeevan","year":"2025","unstructured":"Rajeevan A, Olakkal V, Balakrishnan M, Chakrabarty D, Charon F, Noordermeer D, Kotak S (2025) Aurora A regulates the material property of spindle poles to orchestrate nuclear organization at mitotic exit. EMBO J 44:6797\u20136831","journal-title":"EMBO J"},{"key":"928_CR48","doi-asserted-by":"publisher","first-page":"1422","DOI":"10.1038\/ncb3241","volume":"17","author":"J Roostalu","year":"2015","unstructured":"Roostalu J, Cade NI, Surrey T (2015) Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module. Nat Cell Biol 17:1422\u20131434","journal-title":"Nat Cell Biol"},{"key":"928_CR49","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/j.cell.2005.10.029","volume":"123","author":"OS Rosenberg","year":"2005","unstructured":"Rosenberg OS, Deindl S, Sung RJ, Nairn AC, Kuriyan J (2005) Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme. Cell 123:849\u2013860","journal-title":"Cell"},{"key":"928_CR50","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1126\/science.1157425","volume":"320","author":"A Seki","year":"2008","unstructured":"Seki A, Coppinger JA, Jang CY, Yates JR, Fang G (2008) Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry. Science 320:1655\u20131658","journal-title":"Science"},{"key":"928_CR51","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1021\/acssynbio.7b00248","volume":"7","author":"F Shi","year":"2018","unstructured":"Shi F, Kawano F, Park SE, Komazaki S, Hirabayashi Y, Polleux F, Yazawa M (2018) Optogenetic control of endoplasmic reticulum-mitochondria tethering. ACS Synth Biol 7:2\u20139","journal-title":"ACS Synth Biol"},{"key":"928_CR52","doi-asserted-by":"publisher","first-page":"3819","DOI":"10.1091\/mbc.e13-02-0108","volume":"24","author":"VC Silva","year":"2013","unstructured":"Silva VC, Cassimeris L (2013) Stathmin and microtubules regulate mitotic entry in HeLa cells by controlling activation of both Aurora kinase A and Plk1. Mol Biol Cell 24:3819\u20133831","journal-title":"Mol Biol Cell"},{"key":"928_CR53","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1083\/jcb.201309038","volume":"203","author":"JH Sir","year":"2013","unstructured":"Sir JH, Putz M, Daly O, Morrison CG, Dunning M, Kilmartin JV, Gergely F (2013) Loss of centrioles causes chromosomal instability in vertebrate somatic cells. J Cell Biol 203:747\u2013756","journal-title":"J Cell Biol"},{"key":"928_CR54","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj3944","volume":"375","author":"C So","year":"2022","unstructured":"So C, Menelaou K, Uraji J, Harasimov K, Steyer AM, Seres KB, Bucevicius J, Lukinavicius G, Mobius W, Sibold C et al (2022) Mechanism of spindle pole organization and instability in human oocytes. Science 375:eabj3944","journal-title":"Science"},{"key":"928_CR55","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202106128","volume":"220","author":"N Tavernier","year":"2021","unstructured":"Tavernier N, Sicheri F, Pintard L (2021) Aurora A kinase activation: different means to different ends. J Cell Biol 220:e202106128","journal-title":"J Cell Biol"},{"key":"928_CR56","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1038\/ncb2242","volume":"13","author":"M Toya","year":"2011","unstructured":"Toya M, Terasawa M, Nagata K, Iida Y, Sugimoto A (2011) A kinase-independent role for Aurora A in the assembly of mitotic spindle microtubules in Caenorhabditis elegans embryos. Nat Cell Biol 13:708\u2013714","journal-title":"Nat Cell Biol"},{"key":"928_CR57","doi-asserted-by":"publisher","first-page":"2156","DOI":"10.1016\/j.cub.2005.10.054","volume":"15","author":"MY Tsai","year":"2005","unstructured":"Tsai MY, Zheng Y (2005) Aurora A kinase-coated beads function as microtubule-organizing centers and enhance RanGTP-induced spindle assembly. Curr Biol 15:2156\u20132163","journal-title":"Curr Biol"},{"key":"928_CR58","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1038\/nature14128","volume":"518","author":"P van Bergeijk","year":"2015","unstructured":"van Bergeijk P, Adrian M, Hoogenraad CC, Kapitein LC (2015) Optogenetic control of organelle transport and positioning. Nature 518:111\u2013114","journal-title":"Nature"},{"key":"928_CR59","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202006010","volume":"219","author":"S Watanabe","year":"2020","unstructured":"Watanabe S, Meitinger F, Shiau AK, Oegema K, Desai A (2020) Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix. J Cell Biol 219:e202006010","journal-title":"J Cell Biol"},{"key":"928_CR60","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/ncb3188","volume":"17","author":"M Wieczorek","year":"2015","unstructured":"Wieczorek M, Bechstedt S, Chaaban S, Brouhard GJ (2015) Microtubule-associated proteins control the kinetics of microtubule nucleation. Nat Cell Biol 17:907\u2013916","journal-title":"Nat Cell Biol"},{"key":"928_CR61","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq9549","volume":"11","author":"SS Wong","year":"2025","unstructured":"Wong SS, Monteiro JM, Chang CC, Peng M, Mohamad N, Steinacker TL, Xiao B, Saurya S, Wainman A, Raff JW (2025) Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomes. Sci Adv 11:eadq9549","journal-title":"Sci Adv"},{"key":"928_CR62","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1126\/science.aaa5111","volume":"348","author":"YL Wong","year":"2015","unstructured":"Wong YL, Anzola JV, Davis RL, Yoon M, Motamedi A, Kroll A, Seo CP, Hsia JE, Kim SK, Mitchell JW et al (2015) Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4. Science 348:1155\u20131160","journal-title":"Science"},{"key":"928_CR63","doi-asserted-by":"publisher","first-page":"4351","DOI":"10.1083\/jcb.201702188","volume":"216","author":"LA Wood","year":"2017","unstructured":"Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ (2017) New tools for \u201chot-wiring\u201d clathrin-mediated endocytosis with temporal and spatial precision. J Cell Biol 216:4351\u20134365","journal-title":"J Cell Biol"},{"key":"928_CR64","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1016\/j.cell.2017.05.028","volume":"169","author":"JB Woodruff","year":"2017","unstructured":"Woodruff JB, Ferreira Gomes B, Widlund PO, Mahamid J, Honigmann A, Hyman AA (2017) The centrosome is a selective condensate that nucleates microtubules by concentrating tubulin. Cell 169:1066\u20131077.e10","journal-title":"Cell"},{"key":"928_CR65","doi-asserted-by":"publisher","first-page":"20130459","DOI":"10.1098\/rstb.2013.0459","volume":"369","author":"JB Woodruff","year":"2014","unstructured":"Woodruff JB, Wueseke O, Hyman AA (2014) Pericentriolar material structure and dynamics. Philos Trans R Soc Lond B Biol Sci 369:20130459","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"928_CR66","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1126\/science.aaa3923","volume":"348","author":"JB Woodruff","year":"2015","unstructured":"Woodruff JB, Wueseke O, Viscardi V, Mahamid J, Ochoa SD, Bunkenborg J, Widlund PO, Pozniakovsky A, Zanin E, Bahmanyar S et al (2015) Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Science 348:808\u2013812","journal-title":"Science"},{"key":"928_CR67","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.109785","volume":"27","author":"H Zheng","year":"2024","unstructured":"Zheng H, Zhang Q, Liu X, Shi F, Yang F, Xiang S, Jiang H (2024) Aurora-A condensation mediated by BuGZ aids its mitotic centrosome functions. iScience 27:109785","journal-title":"iScience"},{"key":"928_CR68","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.02667","volume":"3","author":"A Zorba","year":"2014","unstructured":"Zorba A, Buosi V, Kutter S, Kern N, Pontiggia F, Cho YJ, Kern D (2014) Molecular mechanism of Aurora A kinase autophosphorylation and its allosteric activation by TPX2. Elife 3:e02667","journal-title":"Elife"}],"container-title":["EMBO Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1038\/s44319-026-00928-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1038\/s44319-026-00928-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1038\/s44319-026-00928-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T14:02:40Z","timestamp":1789480960000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1038\/s44319-026-00928-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":66,"alternative-id":["928"],"URL":"https:\/\/doi.org\/10.1038\/s44319-026-00928-9","relation":{},"ISSN":["1469-3178"],"issn-type":[{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"30 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 August 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 September 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosure and competing interests statement"}}]}}