{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T11:10:02Z","timestamp":1721041802453},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T00:00:00Z","timestamp":1444089600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Spinal Cord"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1038\/sc.2015.162","type":"journal-article","created":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T09:21:09Z","timestamp":1444123269000},"page":"423-430","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Construction of recombinant adenovirus containing picorna-viral 2A-peptide sequence for the co-expression of neuro-protective growth factors in human umbilical cord blood cells"],"prefix":"10.1038","volume":"54","author":[{"given":"E E","family":"Garanina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y O","family":"Mukhamedshina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I I","family":"Salafutdinov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A P","family":"Kiyasov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L M","family":"Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H J","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Palot\u00e1s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R R","family":"Islamov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A A","family":"Rizvanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,10,6]]},"reference":[{"key":"BFsc2015162_CR1","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1126\/science.1589762","volume":"256","author":"WF Anderson","year":"1992","unstructured":"Anderson WF . Human gene therapy. Science 1992; 256: 808\u2013813.","journal-title":"Science"},{"key":"BFsc2015162_CR2","doi-asserted-by":"publisher","first-page":"355","DOI":"10.2174\/1566523023347742","volume":"2","author":"P de Felipe","year":"2002","unstructured":"de Felipe P . Polycistronic viral vectors. Curr Gene Ther 2002; 2: 355\u2013378.","journal-title":"Curr Gene Ther"},{"key":"BFsc2015162_CR3","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1358\/dnp.2008.21.9.1290817","volume":"21","author":"W Pfutzner","year":"2008","unstructured":"Pfutzner W . Retroviral bicistronic vectors. Drug News Perspect 2008; 21: 473\u2013480.","journal-title":"Drug News Perspect"},{"key":"BFsc2015162_CR4","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1016\/S0958-1669(99)00010-5","volume":"10","author":"E Martinez-Salas","year":"1999","unstructured":"Martinez-Salas E . Internal ribosome entry site biology and its use in expression vectors. Curr Opin Biotechnol 1999; 10: 458\u2013464.","journal-title":"Curr Opin Biotechnol"},{"key":"BFsc2015162_CR5","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/sj.gt.3301667","volume":"9","author":"ET Wong","year":"2002","unstructured":"Wong ET, Ngoi SM, Lee CG . Improved co-expression of multiple genes in vectors containing internal ribosome entry sites (IRESes) from human genes. Gene Therapy 2002; 9: 337\u2013344.","journal-title":"Gene Therapy"},{"key":"BFsc2015162_CR6","doi-asserted-by":"publisher","first-page":"6593","DOI":"10.1093\/nar\/gki958","volume":"33","author":"M Kozak","year":"2005","unstructured":"Kozak M . A second look at cellular mRNA sequences said to function as internal ribosome entry sites. Nucleic Acids Res 2005; 33: 6593\u20136602.","journal-title":"Nucleic Acids Res"},{"key":"BFsc2015162_CR7","doi-asserted-by":"publisher","first-page":"4733","DOI":"10.1073\/pnas.0710650105","volume":"105","author":"BT Baranick","year":"2008","unstructured":"Baranick BT, Lemp NA, Nagashima J, Hiraoka K, Kasahara N, Logg CR . Splicing mediates the activity of four putative cellular internal ribosome entry sites. Proc Natl Acad Sci USA 2008; 105: 4733\u20134738.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsc2015162_CR8","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1016\/j.neurol.2014.09.004","volume":"170","author":"C Serguera","year":"2014","unstructured":"Serguera C, Bemelmans AP . Gene therapy of the central nervous system: general considerations on viral vectors for gene transfer into the brain. Rev Neurol (Paris) 2014; 170: 727\u2013738.","journal-title":"Rev Neurol (Paris)"},{"key":"BFsc2015162_CR9","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1161\/01.CIR.0000061911.47710.8A","volume":"107","author":"TD Henry","year":"2003","unstructured":"Henry TD, Annex BH, McKendall GR, Azrin MA, Lopez JJ, Giordano FJ et al. The VIVA trial: vascular endothelial growth factor in ischemia for vascular angiogenesis. Circulation 2003; 107: 1359\u20131365.","journal-title":"Circulation"},{"key":"BFsc2015162_CR10","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1016\/S0140-6736(02)08937-7","volume":"359","author":"RJ Lederman","year":"2002","unstructured":"Lederman RJ, Mendelsohn FO, Anderson RD, Saucedo JF, Tenaglia AN, Hermiller JB et al. Therapeutic angiogenesis with recombinant fibroblast growth factor-2 for intermittent claudication (the TRAFFIC study): a randomised trial. Lancet 2002; 359: 2053\u20132058.","journal-title":"Lancet"},{"key":"BFsc2015162_CR11","doi-asserted-by":"publisher","first-page":"3759","DOI":"10.1242\/jcs.02483","volume":"118","author":"MR Kano","year":"2005","unstructured":"Kano MR, Morishita Y, Iwata C, Iwasaka S, Watabe T, Ouchi Y et al. VEGF-A and FGF-2 synergistically promote neoangiogenesis through enhancement of endogenous PDGF-B-PDGFRbeta signaling. J Cell Sci 2005; 118: 3759\u20133768.","journal-title":"J Cell Sci"},{"key":"BFsc2015162_CR12","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1258\/ebm.2010.010172","volume":"236","author":"AA Rizvanov","year":"2011","unstructured":"Rizvanov AA, Guseva DS, Salafutdinov II, Kudryashova NV, Bashirov FV, Kiyasov AP et al. Genetically modified human umbilical cord blood cells expressing vascular endothelial growth factor and fibroblast growth factor 2 differentiate into glial cells after transplantation into amyotrophic lateral sclerosis transgenic mice. Exp Biol Med (Maywood) 2011; 236: 91\u201398.","journal-title":"Exp Biol Med (Maywood)"},{"key":"BFsc2015162_CR13","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.neuint.2008.09.011","volume":"53","author":"AA Rizvanov","year":"2008","unstructured":"Rizvanov AA, Kiyasov AP, Gaziziov IM, Yilmaz TS, Kaligin MS, Andreeva DI et al. Human umbilical cord blood cells transfected with VEGF and L(1)CAM do not differentiate into neurons but transform into vascular endothelial cells and secrete neuro-trophic factors to support neuro-genesis-a novel approach in stem cell therapy. Neurochem Int 2008; 53: 389\u2013394.","journal-title":"Neurochem Int"},{"issue":"4","key":"BFsc2015162_CR14","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1016\/j.bbrc.2014.07.132","volume":"451","author":"Daria Guseva","year":"2014","unstructured":"Guseva D, Rizvanov AA, Salafutdinov II, Kudryashova NV, Palot\u00e1s A, Islamov RR . Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo. Biochem Biophys Res Commun 451: 503\u2013509.","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"5","key":"BFsc2015162_CR15","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1089\/hum.2013.172","volume":"25","author":"Hanna Kym\u00e4l\u00e4inen","year":"2014","unstructured":"Kymalainen H, Appelt JU, Giordano FA, Davies AF, Ogilvie CM, Ahmed SG et al. Long-term episomal transgene expression from mitotically stable integration-deficient lentiviral vectors. Hum Gene Ther 25: 428\u2013442.","journal-title":"Human Gene Therapy"},{"key":"BFsc2015162_CR16","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1038\/ng0393-224","volume":"3","author":"S Akli","year":"1993","unstructured":"Akli S, Caillaud C, Vigne E, Stratford-Perricaudet LD, Poenaru L, Perricaudet M et al. Transfer of a foreign gene into the brain using adenovirus vectors. Nat Genet 1993; 3: 224\u2013228.","journal-title":"Nat Genet"},{"key":"BFsc2015162_CR17","doi-asserted-by":"publisher","first-page":"266","DOI":"10.2174\/1566523215666150126122317","volume":"15","author":"RR Islamov","year":"2015","unstructured":"Islamov RR, Rizvanov AA, Mukhamedyarov MA, Salafutdinov II, Garanina EE, Fedotova VY et al. Symptomatic improvement, increased life-span and sustained cell homing in amyotrophic lateral sclerosis after transplantation of human umbilical cord blood cells genetically modified with adeno-viral vectors expressing a neuro-protective factor and a neural cell adhesion molecule. Curr Gene Ther 2015; 15: 266\u2013276.","journal-title":"Curr Gene Ther"},{"key":"BFsc2015162_CR18","doi-asserted-by":"publisher","first-page":"e76117","DOI":"10.1371\/journal.pone.0076117","volume":"8","author":"S Kirchmaier","year":"2013","unstructured":"Kirchmaier S, Lust K, Wittbrodt J . Golden GATEway cloning\u2014a combinatorial approach to generate fusion and recombination constructs. PLoS ONE 2013; 8: e76117.","journal-title":"PLoS ONE"},{"key":"BFsc2015162_CR19","first-page":"219","volume":"263","author":"TS Hawley","year":"2004","unstructured":"Hawley TS, Herbert DJ, Eaker SS, Hawley RG . Multiparameter flow cytometry of fluorescent protein reporters. Methods Mol Biol 2004; 263: 219\u2013238.","journal-title":"Methods Mol Biol"},{"key":"BFsc2015162_CR20","doi-asserted-by":"publisher","first-page":"679","DOI":"10.2174\/138920011796504464","volume":"12","author":"AA Rizvanov","year":"2011","unstructured":"Rizvanov AA, Gulluoglu S, Yalvac ME, Palot\u00e1s A, Islamov RR . RNA interference and amyotrophic lateral sclerosis. Curr Drug Metab 2011; 12: 679\u2013683.","journal-title":"Curr Drug Metab"},{"key":"BFsc2015162_CR21","doi-asserted-by":"publisher","first-page":"291730","DOI":"10.1155\/2013\/291730","volume":"2013","author":"E Minskaia","year":"2013","unstructured":"Minskaia E, Ryan MD . Protein coexpression using FMDV 2A: effect of \u2018linker\u2019 residues. Biomed Res Int 2013; 2013: 291730.","journal-title":"Biomed Res Int"},{"issue":"4","key":"BFsc2015162_CR22","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1007\/s00253-010-2625-0","volume":"87","author":"Thomas Jostock","year":"2010","unstructured":"Jostock T, Dragic Z, Fang J, Jooss K, Wilms B, Knopf HP . Combination of the 2A\/furin technology with an animal component free cell line development platform process. Appl Microbiol Biotechnol 87: 1517\u20131524.","journal-title":"Applied Microbiology and Biotechnology"},{"key":"BFsc2015162_CR23","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1111\/j.1399-3089.2011.00631.x","volume":"18","author":"N Fisicaro","year":"2011","unstructured":"Fisicaro N, Londrigan SL, Brady JL, Salvaris E, Nottle MB, O'Connell PJ et al. Versatile co-expression of graft-protective proteins using 2A-linked cassettes. Xenotransplantation 2011; 18: 121\u2013130.","journal-title":"Xenotransplantation"},{"key":"BFsc2015162_CR24","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00401-002-0630-9","volume":"105","author":"T Kawasaki","year":"2003","unstructured":"Kawasaki T, Oka N, Tachibana H, Akiguchi I, Shibasaki H . Oct6, a transcription factor controlling myelination, is a marker for active nerve regeneration in peripheral neuropathies. Acta Neuropathol. 2003; 105: 203\u2013208.","journal-title":"Acta Neuropathol."},{"key":"BFsc2015162_CR25","doi-asserted-by":"publisher","first-page":"1991","DOI":"10.1002\/glia.22414","volume":"60","author":"D Bataveljic","year":"2012","unstructured":"Bataveljic D, Nikolic L, Milosevic M, Todorovic N, Andjus PR . Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1(G93A) rat model. Glia 2012; 60: 1991\u20132003.","journal-title":"Glia"}],"container-title":["Spinal Cord"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/sc2015162.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/sc2015162","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/sc2015162.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T22:28:54Z","timestamp":1684621734000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/sc2015162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,6]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["BFsc2015162"],"URL":"https:\/\/doi.org\/10.1038\/sc.2015.162","relation":{},"ISSN":["1362-4393","1476-5624"],"issn-type":[{"value":"1362-4393","type":"print"},{"value":"1476-5624","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,6]]},"assertion":[{"value":"2 June 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 July 2015","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 July 2015","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 October 2015","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}