{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:40:57Z","timestamp":1781624457977,"version":"3.54.5"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Data"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNAs (miRNAs) regulate virus replication through multiple mechanisms. Poliovirus causes a highly debilitating disease and though global efforts to eradicate polio have sharply decreased polio incidence, unfortunately three countries (Afghanistan, Nigeria and Pakistan) remain polio-endemic. We hypothesize that understanding the host factors involved in polio replication will identify novel prophylactic and therapeutic targets against polio and related viruses. In this data set, employing genome wide screens of miRNA mimics and inhibitors, we identified miRNAs which significantly suppressed polio replication. Specifically, miR-134 regulates poliovirus replication via modulation of ras-related nuclear protein (RAN), an important component of the nuclear transport system. MiR-134 also inhibited other Picornaviridae viruses including EV71, a growing concern and a high priority for vaccination in Asian countries like China. These findings demonstrate a novel mechanism for miRNA regulation of poliovirus and other Picornaviridae viruses in host cells, and thereby may provide a novel approach in combating infection and a potential approach for the development of anti-Picornaviridae strategies.<\/jats:p>","DOI":"10.1038\/sdata.2017.23","type":"journal-article","created":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T18:34:30Z","timestamp":1488393270000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["MicroRNA screening identifies miR-134 as a regulator of poliovirus and enterovirus 71 infection"],"prefix":"10.1038","volume":"4","author":[{"given":"Nichole Lynn","family":"Orr-Burks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byoung-Shik","family":"Shim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhijeet A.","family":"Bakre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon","family":"Karpilow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph A.","family":"Tripp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,3,1]]},"reference":[{"key":"BFsdata201723_CR1","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1016\/j.vaccine.2015.01.018","volume":"33","author":"J Guo","year":"2015","unstructured":"Guo, J., Bolivar-Wagers, S., Srinivas, N., Holubar, M. & Maldonado, Y. Immunodeficiency-related vaccine-derived poliovirus (iVDPV) cases: a systematic review and implications for polio eradication. Vaccine 33, 1235\u20131242 (2015).","journal-title":"Vaccine"},{"key":"BFsdata201723_CR2","doi-asserted-by":"publisher","first-page":"2649","DOI":"10.1016\/j.vaccine.2009.02.071","volume":"27","author":"P Minor","year":"2009","unstructured":"Minor, P. Vaccine-derived poliovirus (VDPV): Impact on poliomyelitis eradication. Vaccine 27, 2649\u20132652 (2009).","journal-title":"Vaccine"},{"key":"BFsdata201723_CR3","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1017\/S0950268809991555","volume":"138","author":"SS Wong","year":"2010","unstructured":"Wong, S. S., Yip, C. C., Lau, S. K. & Yuen, K. Y. Human enterovirus 71 and hand, foot and mouth disease. Epidemiol Infect 138, 1071\u20131089 (2010).","journal-title":"Epidemiol Infect"},{"key":"BFsdata201723_CR4","doi-asserted-by":"publisher","first-page":"e28","DOI":"10.1038\/cti.2014.24","volume":"3","author":"Z Liang","year":"2014","unstructured":"Liang, Z. & Wang, J. EV71 vaccine, an invaluable gift for children. Clin Transl Immunology 3, e28 (2014).","journal-title":"Clin Transl Immunology"},{"key":"BFsdata201723_CR5","first-page":"281","volume":"116","author":"DP Bartel","year":"2004","unstructured":"Bartel, D. P. MicroRNAs: Genomics, Biogenesis, Mechanism, and Function. Cell Press 116, 281\u2013297 (2004).","journal-title":"Cell Press"},{"key":"BFsdata201723_CR6","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1038\/nrg2290","volume":"9","author":"W Filipowicz","year":"2008","unstructured":"Filipowicz, W., Bhattacharyya, S. N. & Sonenberg, N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nature reviews. Genetics 9, 102\u2013114 (2008).","journal-title":"Nature reviews. Genetics"},{"key":"BFsdata201723_CR7","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1146\/annurev.micro.112408.134243","volume":"64","author":"RL Skalsky","year":"2010","unstructured":"Skalsky, R. L. & Cullen, B. R. Viruses, microRNAs, and host interactions. Annual review of microbiology 64, 123\u2013141 (2010).","journal-title":"Annual review of microbiology"},{"key":"BFsdata201723_CR8","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1101\/gad.1793309","volume":"23","author":"JL Umbach","year":"2009","unstructured":"Umbach, J. L. & Cullen, B. R. The role of RNAi and microRNAs in animal virus replication and antiviral immunity. Genes & development 23, 1151\u20131164 (2009).","journal-title":"Genes & development"},{"key":"BFsdata201723_CR9","doi-asserted-by":"publisher","first-page":"3610","DOI":"10.1093\/bioinformatics\/bti562","volume":"21","author":"X Wang","year":"2005","unstructured":"Wang, X. et al. MicroRNA identification based on sequence and structure alignment. Bioinformatics 21, 3610\u20133614 (2005).","journal-title":"Bioinformatics"},{"key":"BFsdata201723_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.immuni.2007.07.003","volume":"27","author":"S Muller","year":"2007","unstructured":"Muller, S. & Imler, J. L. Dicing with viruses: microRNAs as antiviral factors. Immunity 27, 1\u20133 (2007).","journal-title":"Immunity"},{"key":"BFsdata201723_CR11","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1038\/nature12869","volume":"506","author":"DW Trobaugh","year":"2014","unstructured":"Trobaugh, D. W. et al. RNA viruses can hijack vertebrate microRNAs to suppress innate immunity. Nature 506, 245\u2013248 (2014).","journal-title":"Nature"},{"key":"BFsdata201723_CR12","doi-asserted-by":"publisher","first-page":"9707","DOI":"10.1128\/JVI.00802-13","volume":"87","author":"M Cheng","year":"2013","unstructured":"Cheng, M. et al. High-Throughput Profiling of Alpha Interferon- and Interleukin-28B- Regulated MicroRNAs and Identification of let-7s with Anti-Hepatitis C Virus Activity by Targeting IGF2BP1. Journal of virology 87, 9707\u20139718 (2013).","journal-title":"Journal of virology"},{"key":"BFsdata201723_CR13","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1261\/rna.048744.114","volume":"21","author":"JY Huang","year":"2015","unstructured":"Huang, J. Y. et al. MicroRNA-130a can inhibit hepatitis B virus replication via targeting PGC1alpha and PPARgamma. Rna 21, 385\u2013400 (2015).","journal-title":"Rna"},{"key":"BFsdata201723_CR14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1586\/14760584.4.2.167","volume":"4","author":"AP Ivanov","year":"2005","unstructured":"Ivanov, A. P. ELISA as a possible alternative to the neutralization test for evaluating the immune response to poliovirus vaccines. Expert Review Vaccines 4, 167\u2013172 (2005).","journal-title":"Expert Review Vaccines"},{"key":"BFsdata201723_CR15","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/0928-0197(94)90044-2","volume":"2","author":"R Nibbeling","year":"1994","unstructured":"Nibbeling, R. A poliovirus type-specific IgM antibody-capture enzyme-linked immunosorbent assay for the rapid diagnosis of poliomyelitis. Clinical and Diagnostic Virology 2, 113\u2013126 (1994).","journal-title":"Clinical and Diagnostic Virology"},{"key":"BFsdata201723_CR16","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.1128\/JVI.02143-15","volume":"90","author":"WF Tang","year":"2015","unstructured":"Tang, W. F. et al. Host MicroRNA miR-197 Plays a Negative Regulatory Role in the Enterovirus 71 Infectious Cycle by Targeting the RAN Protein. Journal of virology 90, 1424\u20131438 (2015).","journal-title":"Journal of virology"},{"key":"BFsdata201723_CR17","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1038\/nature03315","volume":"433","author":"LP Lim","year":"2005","unstructured":"Lim, L. P. et al. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 433, 769\u2013773 (2005).","journal-title":"Nature"},{"key":"BFsdata201723_CR18","doi-asserted-by":"publisher","first-page":"5645","DOI":"10.1128\/JVI.02655-12","volume":"87","author":"Z Zheng","year":"2013","unstructured":"Zheng, Z. et al. Human microRNA hsa-miR-296-5p suppresses enterovirus 71 replication by targeting the viral genome. Journal of virology 87, 5645\u20135656 (2013).","journal-title":"Journal of virology"},{"key":"BFsdata201723_CR19","doi-asserted-by":"publisher","first-page":"2518","DOI":"10.1099\/vir.0.2008\/002915-0","volume":"89","author":"M Arita","year":"2008","unstructured":"Arita, M., Wakita, T. & Shimizu, H. Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity. The Journal of general virology 89, 2518\u20132530 (2008).","journal-title":"The Journal of general virology"},{"key":"BFsdata201723_CR20","doi-asserted-by":"publisher","first-page":"e66796","DOI":"10.1371\/journal.pone.0066796","volume":"8","author":"A Bakre","year":"2013","unstructured":"Bakre, A. et al. Identification of Host Kinase Genes Required for Influenza Virus Replication and the Regulatory Role of MicroRNAs. PLoS ONE 8, e66796 (2013).","journal-title":"PLoS ONE"},{"key":"BFsdata201723_CR21","doi-asserted-by":"publisher","first-page":"3259","DOI":"10.1128\/JVI.80.7.3259-3272.2006","volume":"80","author":"CC Burns","year":"2006","unstructured":"Burns, C. C. et al. Modulation of poliovirus replicative fitness in HeLa cells by deoptimization of synonymous codon usage in the capsid region. Journal of virology 80, 3259\u20133272 (2006).","journal-title":"Journal of virology"},{"key":"BFsdata201723_CR22","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1099\/jgv.0.000372","volume":"97","author":"BS Shim","year":"2016","unstructured":"Shim, B. S. et al. MicroRNA-555 has potent antiviral properties against poliovirus. The Journal of general virology 97, 659\u2013668 (2016).","journal-title":"The Journal of general virology"},{"key":"BFsdata201723_CR23","doi-asserted-by":"publisher","first-page":"1694","DOI":"10.1128\/JVI.01464-15","volume":"90","author":"SM van der Sanden","year":"2016","unstructured":"van der Sanden, S. M. et al. Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game. Journal of virology 90, 1694\u20131704 (2016).","journal-title":"Journal of virology"},{"key":"BFsdata201723_CR24","author":"N. Orr-Burks","year":"2017","unstructured":"Orr-Burks, N., Shim, B., Wu, W., Bakre, A., & Tripp, R. NCBI PubChem BioAssay 1224906 (2017)"},{"key":"BFsdata201723_CR25","author":"N. Orr-Burks","year":"2017","unstructured":"Orr-Burks, N., Shim, B., Wu, W., Bakre, A., & Tripp, R. NCBI PubChem BioAssay 1224851 (2017)"}],"container-title":["Scientific Data"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/sdata201723.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/sdata201723","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/sdata201723.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T02:55:01Z","timestamp":1671764101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/sdata201723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,1]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,12,19]]}},"alternative-id":["BFsdata201723"],"URL":"https:\/\/doi.org\/10.1038\/sdata.2017.23","relation":{"references":[{"id-type":"uri","id":"https:\/\/pubchem.ncbi.nlm.nih.gov\/bioassay\/1224906","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/pubchem.ncbi.nlm.nih.gov\/bioassay\/1224851","asserted-by":"subject"}]},"ISSN":["2052-4463"],"issn-type":[{"value":"2052-4463","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,1]]},"assertion":[{"value":"11 August 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 December 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 March 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"170023"}}