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Here we report on a large-scale proteomic assessment of the venom of <jats:italic>Loxosceles intermedia<\/jats:italic>, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10\u2009kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4\u2009h), trypsin (18\u2009h), pepsin (18\u2009h), and chymotrypsin (18\u2009h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently <jats:italic>de novo<\/jats:italic> sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. 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