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Dynein's functional diversity depends on co-factors that regulate its subcellular localization, interaction with cargo, and motor activity. The ubiquitous co-factor nuclear distribution gene E (NudE) is implicated in many of dynein's functions, and mutations in NudE cause the brain developmental disease microcephaly. To identify genetic interactors of the <jats:italic>Caenorhabditis elegans<\/jats:italic> NudE homolog <jats:italic>nud-2<\/jats:italic>, we performed a genome-wide RNAi screen with the null allele <jats:italic>nud-2(ok949)<\/jats:italic>, which compromises dynein function but leaves animals viable and fertile. Using bacterial feeding to deliver dsRNAs in a 96-well liquid format and a semi-automated fluorescence microscopy approach for counting parents and progeny, we screened 19762 bacterial clones and identified 38 genes whose inhibition caused enhanced lethality in <jats:italic>nud-2(ok949)<\/jats:italic> relative to the <jats:italic>nud-2(+)<\/jats:italic> control. Further study of these genes, many of which participate in cell division, promises to provide insight into the function and regulation of dynein.<\/jats:p>","DOI":"10.1038\/sdata.2018.47","type":"journal-article","created":{"date-parts":[[2018,3,20]],"date-time":"2018-03-20T14:19:40Z","timestamp":1521555580000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A genome-scale RNAi screen for genetic interactors of the dynein co-factor nud-2 in Caenorhabditis elegans"],"prefix":"10.1038","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1062-2498","authenticated-orcid":false,"given":"Helder","family":"Rocha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 F.","family":"Maia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reto","family":"Gassmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,3,20]]},"reference":[{"key":"BFsdata201847_CR1","doi-asserted-by":"publisher","first-page":"2319","DOI":"10.1242\/jcs.115030","volume":"126","author":"MAM Franker","year":"2013","unstructured":"Franker, M. 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