{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T17:26:45Z","timestamp":1751822805059},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":4416,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effect of reducing reagents on \u03c9\u2010conotoxin GVIA (\u03c9\u2010CgTX) inhibition of the release of [<jats:sup>3<\/jats:sup>H]\u2010acetylcholine ([<jats:sup>3<\/jats:sup>H]\u2010ACh) induced by tityustoxin, K<jats:sup>+<\/jats:sup> 50 m<jats:sc>m<\/jats:sc> and electrical stimulation was investigated in rat brain cortical slices.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In cortical slices the inhibition of tityustoxin or electrically\u2010stimulated [<jats:sup>3<\/jats:sup>H]\u2010ACh release by \u03c9\u2010CgTX was dramatically increased by reducing reagents ascorbate or \u03b2\u2010mercaptoethanol. Dehydroascorbic acid did not substitute for ascorbate<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Depolarization induced by K<jats:sup>+<\/jats:sup> 50 m<jats:sc>m<\/jats:sc> caused [<jats:sup>3<\/jats:sup>H]\u2010ACh release from cortical slices which was not inhibited by \u03c9\u2010CgTX, even in the presence of ascorbate.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the guinea\u2010pig myenteric plexus, \u03c9\u2010CgTX inhibition of the tityustoxin induced release of [<jats:sup>3<\/jats:sup>H]\u2010ACh was independent of ascorbate.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>It is suggested that N\u2010type\u2010like calcium channels in guinea\u2010pig myenteric plexus may have pharmacological\/biochemical diversity from similar channels of rat cerebral cortex.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (1997) <jats:bold>120<\/jats:bold>, 88\u201392; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0700873\">10.1038\/sj.bjp.0700873<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0700873","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T16:03:05Z","timestamp":1156521785000},"page":"88-92","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Different effects of reducing agents on \u03c9\u2010conotoxin GVIA inhibition of [<sup>3<\/sup>H]\u2010acetylcholine release from rat cortical slices and guinea\u2010pig myenteric plexus"],"prefix":"10.1111","volume":"120","author":[{"given":"T A A","family":"Casali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Gomez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Moraes\u2010Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Romano\u2010Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A M","family":"Prado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M V","family":"Gomez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(95)00019-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"14738","DOI":"10.1016\/S0021-9258(18)77174-3","article-title":"Subunits of purified calcium channels: \u03b12\u03b4 are encoded by the same gene","volume":"265","author":"De Jongh K.S.","year":"1990","journal-title":"J. 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