{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:01:29Z","timestamp":1773421289225,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":4416,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Increasing the medium osmolality, with a non\u2010ionic osmoticant, from control (289 mOsm) to 319 mOsm or 344 mOsm in the lumbrical muscle cell of the mouse, resulted in a depolarization of the membrane potential (V<jats:sub>m<\/jats:sub>) of 5.9 mV and 10.9 mV, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In control medium, the blockers of chloride related cotransport bumetanide and furosemide, induced a hyperpolarization of \u22123.6 and \u22123.0 mV and prevented the depolarization due to hypertonicity. When bumetanide was added in hypertonic media V<jats:sub>m<\/jats:sub> fully repolarized to control values.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In a medium of 266 mOsm, the hyperpolarization by bumetanide was absent.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>At 344 mOsm the half\u2010maximal effective concentration (IC<jats:sub>50<\/jats:sub>) was 0.5 \u03bc<jats:sc>m<\/jats:sc> for bumetanide and 21 \u03bc<jats:sc>m<\/jats:sc> for furosemide.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In solutions containing 1.25 m<jats:sc>m<\/jats:sc> sodium the depolarization by hypertonicity was reduced to 2.3 mV.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Reducing chloride permeability, by anthracene 9 carboxylic acid (9\u2010AC) in 289 mOsm, induced a small but significant hyperpolarization of \u22122.6 mV. Increasing medium osmolality to 344 mOsm enlarged this hyperpolarization significantly to \u22127.6 mV.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In a solution of 344 mOsm containing 100 \u03bc<jats:sc>m<\/jats:sc> ouabain, the bumetanide\u2010induced hyperpolarization of V<jats:sub>m<\/jats:sub> was absent.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results indicate that a Na\u2010K\u20102Cl cotransporter is present in mouse lumbrical muscle fibre and that its contribution to V<jats:sub>m<\/jats:sub> is dependent on medium osmolality.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (1997) <jats:bold>120<\/jats:bold>, 39\u201344; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0700887\">10.1038\/sj.bjp.0700887<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0700887","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T16:03:05Z","timestamp":1156521785000},"page":"39-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["The influence of bumetanide on the membrane potential of mouse skeletal muscle cells in isotonic and hypertonic media"],"prefix":"10.1111","volume":"120","author":[{"given":"H G J","family":"Van Mil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R J","family":"Geukes Foppen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Siegenbeek van Heukelom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9685-8_7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017582"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01868010"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870526"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1965.sp007574"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019536"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017728"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.260.1.C122"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012220"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8047888"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.55.2.254"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1985.65.3.760"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.4.C869"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-77124-8_2"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1994.267.3.E343"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.2.315"},{"key":"e_1_2_6_18_1","first-page":"18","article-title":"Cell volume: a second message in regulation of cellular function","volume":"10","author":"Lang F.","year":"1995","journal-title":"News Physiol. 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