{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T03:45:34Z","timestamp":1771472734593,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T00:00:00Z","timestamp":1234224000000},"content-version":"vor","delay-in-days":4423,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Cantharidin, an inhibitor of protein phosphatase types 1 (PP1) and 2A (PP2A), increased basal tone of bovine isolated coronary artery rings (CARs) with and without endothelium in a time\u2010and concentration\u2010dependent manner with pEC<jats:sub>50<\/jats:sub> values of about 5.1 and 5.2, respectively, for both preparations.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>\u03b2\u2010Adrenoceptor stimulation with isoprenaline (Iso; 0.03\u2013100 \u03bcm) or inhibition of phosphodiesterase activity by 3\u2010isobutyl\u2010l\u2010methylxanthine (IBMX; 10\u20131000 \u03bcm), respectively, relaxed CARs precontracted with KC1 (75 mm). CARs with and without endothelium showed no difference in the relaxing response to Iso and IBMX, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cantharidin (3 \u03bcm) attenuated vasorelaxation induced by Iso (0.03\u2013100 \u03bcm) in CARs with and without endothelium in a time\u2010dependent manner, whereas vasorelaxation induced by IBMX (10\u20131000 \u03bcm) was not attenuated by 3 \u03bcM cantharidin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cantharidin (3 \u03bcm) did not affect cyclic AMP content in bovine cultured vascular cells, i.e. coronary artery smooth muscle cells (BCs), aortic endothelial cells (BAECs) and aortic smooth muscle cells (BASMCs), either under basal conditions, after \u03b2\u2010adrenoceptor stimulation (Iso) or inhibition of phosphodiesterase activity (IBMX), respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cantharidin inhibited protein phosphatase activity in homogenates from bovine coronary artery rings with a pIC<jats:sub>50<\/jats:sub> of about 6.0. In homogenates of bovine cultured vascular cells pIC<jats:sub>50<\/jats:sub> values of cantharidin amounted to about 6.5 for BCs, 6.7 for BAECs and 6.7 for BASMCs, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>It was concluded that cantharidin differently affects vasorelaxation due to stimulation of [3\u2010adrenoceptors (Iso) or inhibition of phosphodiesterase activity (IBMX), respectively. The attenuation of \u03b2\u2010adrenoceptor\u2010mediated vasorelaxation by phosphatase inhibition is not due to diminished adenosine 3\u2032: 5\u2032\u2010cyclic monophosphate (cyclic AMP) generation but could be evidence for different subcellular compartments of cyclic AMP.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1038\/sj.bjp.0700929","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T16:07:44Z","timestamp":1156522064000},"page":"421-428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The effect of the protein phosphatases inhibitor cantharidin on \u03b2\u2010adrenoceptor\u2010mediated vasorelaxation"],"prefix":"10.1111","volume":"120","author":[{"given":"J\u00f6rg","family":"Knapp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Bokn\u00edk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Linck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hartmut","family":"Luss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank U.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter Nacke Joachim","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ute","family":"Vahlensieck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelm","family":"Schmitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb17508.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)90768-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/27.2.312"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44447-4"},{"key":"e_1_2_6_7_1","first-page":"692","article-title":"5\u2010Hydroxytryptamine1\u2010like receptors linked to increases in intracellular calcium concentration and inhibition of cyclic AMP accumulation in cultured vascular smooth muscle cells derived from bovine basilar artery","volume":"266","author":"Ebersole B.J.","year":"1993","journal-title":"J. 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