{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:15:35Z","timestamp":1758816935899},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,17]],"date-time":"2009-02-17T00:00:00Z","timestamp":1234828800000},"content-version":"vor","delay-in-days":4371,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Guanosine 3\u2032:5\u2032\u2010cyclic monophosphate (cyclic GMP) is an important second messenger mediating the effects of nitric oxide (NO) and natriuretic peptides. Cyclic GMP pathways regulate several aspects of lung pathophysiology in a number of airway cells. The regulation of this system has not been extensively studied in pulmonary epithelial tissue.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We have studied the production of cyclic GMP by suspensions of ovine tracheal epithelial cells in response to activators of soluble guanylyl cyclase (sodium nitroprusside (SNP) and S\u2010nitroso\u2010N\u2010acetyl\u2010penicillamine (SNAP) and particulate guanylyl cyclase (atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), C\u2010type natriuretic peptide (CNP) and <jats:italic>E. coli<\/jats:italic> heat stable enterotoxin (STa)).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both 10<jats:sup>\u22127<\/jats:sup>10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> and 10<jats:sup>\u22127<\/jats:sup>10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> SNAP generated a concentration\u2010dependent marked elevation in cyclic GMP production when incubated with 10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX) (both greater than 25\u00d7baseline values with highest drug concentration).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The increase in production of cyclic GMP in response to 10<jats:sup>\u22126<\/jats:sup> <jats:sc>m<\/jats:sc> SNP and 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> SNAP was markedly inhibited by both 5\u00d710<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> haemoglobin (102% and 92% inhibition) and 5\u00d710<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> methylene blue (82% and 84% inhibition).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The increase in cyclic GMP in response to 10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> SNP was measured following co\u2010incubation with the phosphodiesterase inhibitors 10<jats:sup>\u22127<\/jats:sup>10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> IBMX, 10<jats:sup>\u22127<\/jats:sup>10<jats:sup>\u22124<\/jats:sup> <jats:sc>m<\/jats:sc> milrinone and 10<jats:sup>\u22127<\/jats:sup>10<jats:sup>\u22124<\/jats:sup> <jats:sc>m<\/jats:sc> SKF 96231. Only 10<jats:sup>\u22124<\/jats:sup>10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> IBMX significantly increased cyclic GMP levels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cyclic GMP production was also significantly elevated from baseline by 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> ANP, 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> BNP, 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> CNP and 200 iu ml<jats:sup>\u22121<\/jats:sup> of <jats:italic>E. coli<\/jats:italic> STa toxin in the presence of 10<jats:sup>\u22123<\/jats:sup> <jats:sc>m<\/jats:sc> IBMX. Increases with these natriuretic peptides and STa toxin were smaller in magnitude (24 fold) than those seen with SNP and SNAP. CNP was the most potent of the natriuretic peptides studied suggesting type B membrane bound guanylate cyclase is the predominant form expressed.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that ovine tracheal epithelial cells contain active guanylyl cyclases. The more marked response to SNP and SNAP than to natriuretic peptides suggests that soluble guanylyl cyclase predominates.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1997) <jats:bold>120<\/jats:bold>, 1249\u20131254; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701040\">10.1038\/sj.bjp.0701040<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701040","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T16:13:16Z","timestamp":1156522396000},"page":"1249-1254","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Regulation of guanosine 3\u2032:5\u2032\u2010cyclic monophosphate in ovine tracheal epithelial cells"],"prefix":"10.1111","volume":"120","author":[{"given":"Simon P.","family":"Range","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine D.","family":"Holland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham P.","family":"Basten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Knox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,17]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"R57","article-title":"ANP inhibits NaCl absorption and elicits choloride secretion in porcine colon: evidence for cGMP and Ca mediation","volume":"34","author":"ARGENZIO R.A.","year":"1993","journal-title":"Am. 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