{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:44:11Z","timestamp":1771634651053,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T00:00:00Z","timestamp":1234224000000},"content-version":"vor","delay-in-days":4242,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>ATP has previously been shown to act as a sympathetic cotransmitter in the rat kidney. The present study analyses the question of whether postganglionic sympathetic nerve endings in the kidney possess P<jats:sub>2<\/jats:sub>\u2010receptors which modulate noradrenaline release. Rat kidneys were perfused with Krebs\u2010Henseleit solution containing the noradrenaline uptake blockers cocaine and corticosterone and the \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor antagonist rauwolscine. The renal nerves were electrically stimulated, in most experiments by 30 pulses applied at 1 Hz. The outflow of endogenous noradrenaline (or, in some experiments, of ATP and lactate dehydrogenase) as well as the perfusion pressure were measured simultaneously.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The P<jats:sub>2<\/jats:sub>\u2010receptor agonist adenosine\u20105\u2032\u2010O\u2010(3\u2010thiotriphosphate) (ATP\u03b3S, 3\u201330 \u03bc<jats:sc>M<\/jats:sc>) reduced the renal nerve stimulation (RNS)\u2010induced outflow of noradrenaline (estimated EC<jats:sub>50<\/jats:sub>=8 \u03bc<jats:sc>M<\/jats:sc>). The P<jats:sub>2<\/jats:sub>\u2010receptor antagonist cibacron blue 3GA (30 \u03bc<jats:sc>M<\/jats:sc>) shifted the concentration\u2010inhibition curve for ATP\u03b3S to the right (apparent p<jats:italic>K<\/jats:italic><jats:sub>B<\/jats:sub> value 4.7).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cibacron blue 3GA (3\u201330 \u03bc<jats:sc>M<\/jats:sc>) and its isomer reactive blue 2 (3\u201330 \u03bc<jats:sc>M<\/jats:sc>) significantly increased RNS\u2010induced outflow of noradrenaline in the presence of the P<jats:sub>1<\/jats:sub>\u2010receptor antagonist 8\u2010(<jats:italic>p<\/jats:italic>\u2010sulphophenyl)theophylline (8\u2010SPT, 100 \u03bc<jats:sc>M<\/jats:sc>) by about 70% and 90%, respectively. The P<jats:sub>2<\/jats:sub>\u2010receptor antagonist suramin (30\u2013300 \u03bc<jats:sc>M<\/jats:sc>) only tended to enhance RNS\u2010induced outflow of noradrenaline. When the nerves were stimulated by short pulse trains consisting of 6 pulses applied at 100 Hz (conditions under which autoinhibition is inoperative), reactive blue 2 did not affect the RNS\u2010induced outflow of noradrenaline.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>RNS (120 pulses applied at 4 Hz) induced the outflow of ATP but not of the cytoplasmatic enzyme lactate dehydrogenase.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ATP\u03b3S (3\u201330 \u03bc<jats:sc>M<\/jats:sc>) concentration\u2010dependently reduced pressor responses to RNS at 1 Hz. Cibacron blue 3GA, reactive blue 2 as well as suramin also reduced pressor responses to RNS (maximally by 50 to 70%).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>This study in rat isolated kidney, in which the release of endogenous noradrenaline was measured, demonstrates that renal sympathetic nerves possess prejunctional P<jats:sub>2<\/jats:sub>\u2010receptors that mediate inhibition of transmitter release. These prejunctional P<jats:sub>2<\/jats:sub>\u2010receptors are activated by endogenous ligands, most likely ATP, released upon nerve activity. Both, P<jats:sub>2<\/jats:sub>\u2010receptor agonists and P<jats:sub>2<\/jats:sub>\u2010receptor antagonists reduced pressor responses to RNS either by inhibiting transmitter release or by blocking postjunctional vasoconstrictor P<jats:sub>2<\/jats:sub>\u2010receptors.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1038\/sj.bjp.0701259","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T17:12:11Z","timestamp":1156525931000},"page":"1255-1262","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["P<sub>2<\/sub>\u2010receptor modulation of noradrenergic neurotransmission in rat kidney"],"prefix":"10.1111","volume":"121","author":[{"given":"Christine","family":"Bohmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivar","family":"von K\u00fcgelgen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Christian Rump","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00169185"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.25.6.1224"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90785-4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13472.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00167445"},{"key":"e_1_2_5_7_1","first-page":"A2119","article-title":"Extracellular ATP in the human kidney","volume":"7","author":"Bohmann C.","year":"1996","journal-title":"J. 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