{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T19:51:12Z","timestamp":1721591472981},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T00:00:00Z","timestamp":1233792000000},"content-version":"vor","delay-in-days":4175,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Block by philanthotoxin\u2010343 (PhTX\u2010343), a neurotoxin from wasps, of ionic currents mediated through neuronal nicotinic acetylcholine (ACh) receptor\/channels was characterized in rat phaeochromocytoma PC12 cells, by use of whole cell voltage\u2010clamp techniques.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the cells held at \u221260\u2003mV, PhTX\u2010343 at 0.1 and 1\u2003\u03bc<jats:sc>M<\/jats:sc> inhibited an inward current activated by 100\u2003\u03bc<jats:sc>M<\/jats:sc> ACh. The current inhibition was relieved by depolarizing steps, and augmented at negative potentials, suggesting that PhTX\u2010343 blocks the channel in a voltage\u2010dependent manner. Joro spider toxin\u20103 (JSTX\u20103) also exerted voltage\u2010dependent inhibition of ACh\u2010activated currents in a similar concentration range, but argiotoxin636 did not affect the currents.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Analysis of the current decay during hyperpolarizing steps indicated that the current inhibition by 100\u2003n<jats:sc>M<\/jats:sc> PhTX\u2010343 develops in an order of several hundreds of milliseconds. On the other hand, the recovery from the current inhibition during depolarizing steps developed in an order of about 100\u2003ms.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results suggest that PhTX\u2010343 blocks neuronal nicotinic receptor channels in PC12 cells at concentrations lower than those required for channel block in non\u2010mammalian cells, and the block exhibits clear voltage\u2010dependence. Estimated from the voltage\u2010dependence, the binding site of PhTX\u2010343 may be located near the outer mouth of the channel.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1997) <jats:bold>122<\/jats:bold>, 379\u2013385; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701373\">10.1038\/sj.bjp.0701373<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701373","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:14:01Z","timestamp":1106939641000},"page":"379-385","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Potent and voltage\u2010dependent block by philanthotoxin\u2010343 of neuronal nicotinic receptor\/channels in PC12 cells"],"prefix":"10.1111","volume":"122","author":[{"given":"Min","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Nakazawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhide","family":"Inou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014714"},{"key":"e_1_2_5_3_1","first-page":"764","article-title":"Structure\u2010activity relationship of philanthotoxin analogs and polyamines on N\u2010methyl\u2010D\u2010aspartate and nicotinic acetylcholine receptors","volume":"254","author":"Anis N.","year":"1990","journal-title":"J. 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