{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:38:56Z","timestamp":1762270736078},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T00:00:00Z","timestamp":1233792000000},"content-version":"vor","delay-in-days":4114,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Glutamate receptor activation has been previously shown to result in mitochondrial depolarization and activation of the mitochondrial permeability transition pore in cultured neurones. In this study, we characterized the effects of two putative permeability transition inhibitors, namely trifluoperazine and dibucaine, on mitochondrial depolarization in rat intact, cultured forebrain neurones.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Permeability transition was monitored by following mitochondrial depolarization in neurones loaded with the mitochondrial membrane potential\u2010sensitive fluorescent indicator, JC\u20101. Trifluoperazine (10\u201320\u2003\u03bc<jats:sc>M<\/jats:sc>) and dibucaine (50\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>) inhibited or delayed the onset of glutamate\u2010induced permeability transition.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We also investigated the effects of trifluoperazine and dibucaine on neuronal recovery from glutamate\u2010induced Ca<jats:sup>2+<\/jats:sup> loads. Trifluoperazine affected Ca<jats:sup>2+<\/jats:sup> recovery in a manner similar to the mitochondrial Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchange inhibitor, CGP\u201037157, while dibucaine had no apparent effect on Ca<jats:sup>2+<\/jats:sup> recovery. Therefore, inhibition of permeability transition does not appear to be involved in Ca<jats:sup>2+<\/jats:sup> recovery from glutamate\u2010induced Ca<jats:sup>2+<\/jats:sup> loads.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Trifluoperazine and dibucaine did not inhibit [<jats:sup>3<\/jats:sup>H]\u2010dizocilpine binding at the concentrations that prevented mitochondrial depolarization.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These studies suggest that trifluoperazine and dibucaine inhibit permeability transition in intact neurones. Trifluoperazine also appears to inhibit mitochondrial Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchange. These drugs should prove to be valuable tools in the further study of the role of mitochondrial permeability transition in glutamate\u2010induced neuronal death.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1997) <jats:bold>122<\/jats:bold>, 803\u2013808; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701442\">10.1038\/sj.bjp.0701442<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701442","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:15:39Z","timestamp":1106939739000},"page":"803-808","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Trifluoperazine and dibucaine\u2010induced inhibition of glutamate\u2010induced mitochondrial depolarization in rat cultured forebrain neurones"],"prefix":"10.1111","volume":"122","author":[{"given":"Kari R.","family":"Hoyt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terre A.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian J.","family":"Reynolds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,5]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00959-3"},{"key":"e_1_2_4_3_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/BF00762735","article-title":"Recent progress on regulation of the mitochondrial permeability transition pore; a cyclosporin\u2010sensitive pore in the inner mitochondrial membrane","volume":"26","author":"Bernardi P.","year":"1994","journal-title":"J. Bioenerg. Biomemb."},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-04-01324.1996"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90084-5"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2006.01830.x"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00050a027"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89700-9"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90162-6"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199304000-00013"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.9808"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.14.6368"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-10-06377.1995"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3070093"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.2.C313"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-05-01993.1993"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68051836.x"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80316-1"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01139-8"},{"key":"e_1_2_4_20_1","first-page":"140","article-title":"Glutamate\u2010induced destabilization of intracellular calcium concentration homeostasis in cultured cerebellar granule cells: role of mitochondria in calcium buffering","volume":"47","author":"Kiedrowski L.","year":"1995","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_4_21_1","first-page":"454","article-title":"Selective binding of antipsychotics and other psychoactive agents to the calcium\u2010dependent activator of cyclic nucleotide phosphodiesterase","volume":"208","author":"Levin R.M.","year":"1979","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90124-H"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-01-00283.1990"},{"key":"e_1_2_4_24_1","first-page":"993","article-title":"Cyclosporin A delays mitochondrial depolarization induced by N\u2010methyl\u2010d\u2010Aspartate in cortical neurons \u2013 evidence of the mitochondrial permeability transition","volume":"75","author":"Nieminen A.L.","year":"1996","journal-title":"Neuroscience"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3070099"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00232a015"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-05-03318.1995"},{"key":"e_1_2_4_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1988.tb16552.x"},{"key":"e_1_2_4_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7744"},{"key":"e_1_2_4_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1991.tb02074.x"},{"key":"e_1_2_4_31_1","first-page":"311","volume-title":"Goodman and Gilman's The Pharmacological Basis of Therapeutics","author":"Ritchie J.","year":"1990"},{"key":"e_1_2_4_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(87)90347-2"},{"key":"e_1_2_4_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1483"},{"key":"e_1_2_4_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00004.2005"},{"key":"e_1_2_4_35_1","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1152\/jn.1994.72.6.2563","article-title":"Glutamate\u2010induced intracellular acidification of cultured hippocampal neurons demonstrates altered energy metabolism resulting from Ca2+ loads","volume":"11","author":"Wang G.J.","year":"1994","journal-title":"J. Neurophysiol."},{"key":"e_1_2_4_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.3.1611"},{"key":"e_1_2_4_37_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021839"},{"key":"e_1_2_4_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-02-01318.1995"},{"key":"e_1_2_4_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-18-05688.1996"},{"key":"e_1_2_4_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(95)00003-A"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0701442","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0701442","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0701442","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T15:27:57Z","timestamp":1698420477000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0701442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1997,11]]}},"alternative-id":["10.1038\/sj.bjp.0701442"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0701442","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11]]},"assertion":[{"value":"2009-02-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}