{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T10:44:11Z","timestamp":1698576251682},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T00:00:00Z","timestamp":1233792000000},"content-version":"vor","delay-in-days":4114,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA) on the ionic currents of rat carotid body type I cells were investigated by use of whole\u2010cell and outside\u2010out patch clamp techniques.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>NDGA (5\u201350\u2003\u03bc<jats:sc>M<\/jats:sc>) produced a concentration\u2010dependent inhibition of whole\u2010cell K<jats:sup>+<\/jats:sup> currents at all activating test potentials (holding potential \u221270\u2003mV). The time\u2010course of the inhibition was also concentration\u2010dependent and the effects of NDGA were only reversible following brief periods of exposure (&lt;2\u2003min). Another lipoxygenase inhibitor, phenidone (5\u2003\u03bc<jats:sc>M<\/jats:sc>), was without effect on whole\u2010cell K<jats:sup>+<\/jats:sup> currents in carotid body type I cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>NDGA (5\u201350\u2003\u03bc<jats:sc>M<\/jats:sc>) also inhibited whole\u2010cell Ca<jats:sup>2+<\/jats:sup> channel currents (recorded with Ba<jats:sup>2+<\/jats:sup> as charge carrier) in a concentration\u2010dependent manner.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Isolation of voltage\u2010gated K<jats:sup>+<\/jats:sup> channels by use of high [Mg<jats:sup>2+<\/jats:sup>] (6\u2003m<jats:sc>M<\/jats:sc>), low [Ca<jats:sup>2+<\/jats:sup>] (0.1\u2003m<jats:sc>M<\/jats:sc>) solutions revealed a direct inhibition of the voltage\u2010sensitive component of the whole\u2010cell K<jats:sup>+<\/jats:sup> current by NDGA (50\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In excised, outside\u2010out patches NDGA (20\u201350\u2003\u03bc<jats:sc>M<\/jats:sc>) increased large conductance, Ca<jats:sup>2+<\/jats:sup> activated K<jats:sup>+<\/jats:sup> channel activity approximately 10 fold, an effect which could be reversed by either tetraethylammonium (10\u2003m<jats:sc>M<\/jats:sc>) or charybdotoxin (30\u2003n<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>It is concluded that NDGA activates maxi\u2010K<jats:sup>+<\/jats:sup> channels in carotid body type I cells and over the same concentration range inhibits voltage\u2010sensitive K<jats:sup>+<\/jats:sup> and Ca<jats:sup>2+<\/jats:sup> channels. The inhibition of whole cell K<jats:sup>+<\/jats:sup> currents seen is most likely due to a combination of direct inhibition of the voltage\u2010sensitive K<jats:sup>+<\/jats:sup> current and indirect inhibition of maxi\u2010K<jats:sup>+<\/jats:sup> channel activity through blockade of Ca<jats:sup>2+<\/jats:sup> channels.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1997) <jats:bold>122<\/jats:bold>, 923\u2013929; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701452\">10.1038\/sj.bjp.0701452<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701452","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:15:39Z","timestamp":1106939739000},"page":"923-929","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Multiple effects of nordihydroguaiaretic acid on ionic currents in rat isolated type I carotid body cells"],"prefix":"10.1111","volume":"122","author":[{"given":"C. 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