{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:24:01Z","timestamp":1758817441733},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T00:00:00Z","timestamp":1234224000000},"content-version":"vor","delay-in-days":4089,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The aim of this study was first, to characterize \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor subtypes in human and rat pregnant myometrium and second, to investigate the possibility of a differential expression of the putative subtypes according to the stage of pregnancy.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In both species, specific [<jats:sup>3<\/jats:sup>H]\u2010rauwolscine binding was inhibited by five different compounds with an order of affinity characteristic of the one described for \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptors (yohimbineclonidine&gt;noradrenaline&gt;phenylephrine&gt;propranolol). Binding affinities (p<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>) for the compounds tested were, in human and rat, respectively: 7.63 and 8.93 for yohimbine, 6.91 and 8.71 for clonidine, 6.23 and 6.09 for noradrenaline, 5.37 and 5.73 for phenylephrine, 4.64 and 4.72 for propranolol.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>By use of non\u2010linear iterative curve fitting procedures and by fitting the data to a two\u2010site model, analysis of [<jats:sup>3<\/jats:sup>H]\u2010rauwolscine inhibition binding curves performed in the presence of oxymetazoline (\u03b1<jats:sub>2A<\/jats:sub>\u2010selective), ARC239, prazosin or chlorpromazine (\u03b1<jats:sub>2B<\/jats:sub>\u2010 and \u03b1<jats:sub>2C<\/jats:sub>\u2010selective) indicated that pregnant human and rat myometrium contain at least two pharmacologically distinct \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor subtypes (\u03b1<jats:sub>2A<\/jats:sub>, \u03b1<jats:sub>2B<\/jats:sub> and\/or \u03b1<jats:sub>2C<\/jats:sub>). RNA blot analysis with probes specific for each cloned human and rat \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor subtype demonstrated that \u03b1<jats:sub>2A<\/jats:sub>\u2010 and \u03b1<jats:sub>2B<\/jats:sub>\u2010subtypes were present in both species but \u03b1<jats:sub>2C<\/jats:sub> seems to be expressed only in human tissues.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the pregnant rat myometrium, subtype selective compounds competition curves revealed a predominant expression of \u03b1<jats:sub>2A<\/jats:sub>\u2010adrenoceptors at mid\u2010pregnancy whereas, at term, \u03b1<jats:sub>2A<\/jats:sub>\u2010 and \u03b1<jats:sub>2B<\/jats:sub>\u2010subtypes density reached approximately the same level (\u03b1<jats:sub>2A<\/jats:sub>\u2003:\u2003\u03b1<jats:sub>2B<\/jats:sub> ratio=73\u2003:\u200327 at mid\u2010pregnancy and=43\u2003:\u200357 at term). In addition, quantification of \u03b1<jats:sub>2A<\/jats:sub>\u2010 and \u03b1<jats:sub>2B<\/jats:sub>\u2010transcripts by densitometry, following data normalization with an oligo(dT)<jats:sub>12\u201318<\/jats:sub> probe, showed a pattern of expression comparable to the one characterized by pharmacological studies.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, these data demonstrate heterogeneity of \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptors in pregnant human and rat myometria and an alteration of the \u03b1<jats:sub>2A<\/jats:sub>\u2010\/\u03b1<jats:sub>2B<\/jats:sub>\u2010subtypes expression pattern during rat pregnancy. Such observations lead us to suggest a multiple role for \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptors in regulating specific functions of myometrium throughout the time course of pregnancy.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1038\/sj.bjp.0701555","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:18:29Z","timestamp":1106939909000},"page":"1732-1738","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Heterogeneity of <i>\u03b1<\/i><sub>2<\/sub>\u2010adrenoceptors in human and rat myometrium and differential expression during pregnancy"],"prefix":"10.1111","volume":"122","author":[{"given":"Rachel","family":"Bouet\u2010Alard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakina","family":"Mhaouty\u2010Kodja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Limon\u2010Boulez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelline","family":"Coudouel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Paul","family":"Maltier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Legrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1990.9.293"},{"key":"e_1_2_6_3_1","first-page":"176","article-title":"Cloning and expression of the alpha\u20102C\u2010adrenergic receptor from the Ok cell line","volume":"45","author":"BLAXALL H.S.","year":"1994","journal-title":"Mol. 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