{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:32:44Z","timestamp":1762144364178},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":4051,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>5\u2010Hydroxytryptamine (5\u2010HT; 1\u2003n<jats:sc>M<\/jats:sc>\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>) concentration\u2010dependently inhibited the amplitude and frequency of spontaneous contractions in longitudinal and circular muscles of the porcine myometrium. The circular muscle (EC<jats:sub>50<\/jats:sub>; 68\u201384\u2003n<jats:sc>M<\/jats:sc>) was more sensitive than the longitudinal muscle (EC<jats:sub>50<\/jats:sub>; 1.3\u20131.44\u2003\u03bc<jats:sc>M<\/jats:sc>) to 5\u2010HT. To characterize the 5\u2010HT receptor subtype responsible for inhibition of myometrial contractility, the effects of 5\u2010HT receptor agonists on spontaneous contractions and of 5\u2010HT receptor antagonists on inhibition by 5\u2010HT were examined in circular muscle preparations.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pretreatment with tetrodotoxin (1\u2003\u03bc<jats:sc>M<\/jats:sc>), propranolol (1\u2003\u03bc<jats:sc>M<\/jats:sc>), atropine (1\u2003\u03bc<jats:sc>M<\/jats:sc>), guanethidine (10\u2003\u03bc<jats:sc>M<\/jats:sc>) or <jats:sc>L<\/jats:sc>\u2010NAME (100\u2003\u03bc<jats:sc>M<\/jats:sc>) failed to change the inhibition by 5\u2010HT, indicating that the inhibition was due to a direct action of 5\u2010HT on the smooth muscle cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>5\u2010CT, 5\u2010MeOT and 8\u2010OH\u2010DPAT mimicked the inhibitory response of 5\u2010HT, and the rank order of the potency was 5\u2010CT&gt;5\u2010HT&gt;5\u2010MeOT&gt;8\u2010OH\u2010DPAT. On the other hand, oxymethazoline, \u03b1\u2010methyl\u20105\u2010HT, 2\u2010methyl\u20105\u2010HT, cisapride, BIMU\u20101, BIMU\u20108, ergotamine and dihydroergotamine had almost no effect on spontaneous contractions, even at 10\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Inhibition by 5\u2010HT was not decreased by either pindolol (1\u2003\u03bc<jats:sc>M<\/jats:sc>), ketanserin (1\u2003\u03bc<jats:sc>M<\/jats:sc>), tropisetron (10\u2003\u03bc<jats:sc>M<\/jats:sc>), MDL72222 (1\u2003\u03bc<jats:sc>M<\/jats:sc>) or GR113808 (10\u2003\u03bc<jats:sc>M<\/jats:sc>), but was antagonized by the following compounds in a competitive manner (with pA<jats:sub>2<\/jats:sub> values in parentheses): methiothepin (8.05), methysergide (7.92), metergoline (7.4), mianserin (7.08), clozapine (7.06) and spiperone (6.86).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Ro 20\u20101724 (20\u2003\u03bc<jats:sc>M<\/jats:sc>) and rolipram (10\u2003\u03bc<jats:sc>M<\/jats:sc>) significantly enhanced the inhibitory response of 5\u2010HT, but neither zaprinast (10\u2003\u03bc<jats:sc>M<\/jats:sc>) nor dipyridamole (10\u2003\u03bc<jats:sc>M<\/jats:sc>) altered the response of 5\u2010HT.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>5\u2010HT (1\u2003n<jats:sc>M<\/jats:sc>\u20131\u2003\u03bc<jats:sc>M<\/jats:sc>) caused a concentration\u2010dependent accumulation of intracellular cyclic AMP in the circular muscle.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>From the present results, the 5\u2010HT receptor, which is functionally correlated with the 5\u2010HT<jats:sub>7<\/jats:sub> receptor, mediates the inhibitory effect of 5\u2010HT on porcine myometrial contractility. This inhibitory response is probably due to an increase in intracellular cyclic AMP through the activation of adenylate cyclase that is positively coupled to 5\u2010HT<jats:sub>7<\/jats:sub> receptors.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>123<\/jats:bold>, 173\u2013182; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701583\">10.1038\/sj.bjp.0701583<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701583","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:16:45Z","timestamp":1106939805000},"page":"173-182","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Involvement of 5\u2010hydroxytryptamine<sub>7<\/sub> receptors in inhibition of porcine myometrial contractility by 5\u2010hydroxytryptamine"],"prefix":"10.1111","volume":"123","author":[{"given":"Takio","family":"Kitazawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Kubo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masami","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Taneike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"895","article-title":"Effect of forskolin on cytosolic Ca2+ level and contraction in vascular smooth muscle","volume":"249","author":"Abe A.","year":"1989","journal-title":"J. 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