{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:05:04Z","timestamp":1760547904069},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T00:00:00Z","timestamp":1234396800000},"content-version":"vor","delay-in-days":3970,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effect of the NSAIDs indomethacin, indoprofen, diclofenac and acetylsalicylic acid on the increase in guanosine 3\u2032:5\u2032\u2010cyclic monophosphate (cyclic GMP) induced by nitric oxide\u2010donor agents was tested in human whole platelets and in platelet crude homogenate.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In whole platelets, indomethacin reduced the increase in cyclic GMP induced by the nitric oxide\u2010donors (NO\u2010donors) sodium nitroprusside (NaNP) and S\u2010nitroso\u2010N\u2010acetylpenicillamine (SNAP) in a dose\u2010dependent way, its IC<jats:sub>50<\/jats:sub> being 13.7 \u03bc<jats:sc>m<\/jats:sc> and 15.8 \u03bc<jats:sc>m<\/jats:sc>, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Of the other cyclooxygenase inhibitors tested, only indoprofen reduced the increase in cyclic GMP induced by both NO\u2010donors in a dose\u2010dependent way (IC<jats:sub>50<\/jats:sub>=32.7 \u03bc<jats:sc>m<\/jats:sc>, NaNP and 25.0 \u03bc<jats:sc>m<\/jats:sc>, SNAP), while acetylsalicylic acid (up to 1000 \u03bc<jats:sc>m<\/jats:sc>) and diclofenac (up to 100 \u03bc<jats:sc>m<\/jats:sc>) were ineffective.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>However, in platelet crude homogenate neither indomethacin nor indoprofen reduced the cyclic GMP production.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Indomethacin (10 \u03bc<jats:sc>m<\/jats:sc>), indoprofen (30 \u03bc<jats:sc>m<\/jats:sc>), diclofenac (100 \u03bc<jats:sc>m<\/jats:sc>) and acetylsalicylic acid (1000 \u03bc<jats:sc>m<\/jats:sc>) showed a comparable efficacy in inhibiting platelet thromboxane B<jats:sub>2<\/jats:sub> (TXB<jats:sub>2<\/jats:sub>) production, suggesting that the inhibitory effect of indomethacin and indoprofen on the increase in cyclic GMP induced by both NO\u2010donors was not mediated by inhibition of cyclooxygenase.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:italic>In vitro<\/jats:italic>, the NSAIDs analysed did not interfere with nitrite production of SNAP.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The unhomogeneous behaviour of NSAIDs on the increase in cyclic GMP induced by NO\u2010donors in whole platelets may contribute to the different pharmacological and toxicological characteristics of the drugs, providing new knowledge on the effect of indomethacin and indoprofen.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>123<\/jats:bold>, 1457\u20131463; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701745\">10.1038\/sj.bjp.0701745<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701745","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:26:01Z","timestamp":1106940361000},"page":"1457-1463","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of some cyclooxygenase inhibitors on the increase in guanosine 3\u2032:5\u2032\u2010cyclic monophosphate induced by NO\u2010donors in human whole platelets"],"prefix":"10.1111","volume":"123","author":[{"given":"Paola","family":"Failli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrica","family":"Cecchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Tosti\u2010Guerra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Mugelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giacomo","family":"Laffi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucilla","family":"Zilletti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Giotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(91)90155-X"},{"key":"e_1_2_5_3_1","first-page":"H308","article-title":"Modulation of platelet function by reactive oxygen metabolites","volume":"267","author":"AMBROSIO G.","year":"1994","journal-title":"Am. J. Physiol."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)90509-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(76)90402-5"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb04766.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19276.x"},{"key":"e_1_2_5_8_1","first-page":"497","article-title":"Inhibition of platelet aggregation by carbon monoxide is mediated by activation of guanylate cyclase","volume":"32","author":"BR\u00dcNE B.","year":"1987","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00213a002"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1986.tb10225.x"},{"key":"e_1_2_5_11_1","first-page":"674","volume-title":"Goodman and Oilman's The Pharmacological Basis of Therapeutics","author":"FLOWER R.J.","year":"1985"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198111123052002"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(96)01017-6"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1016\/0016-5085(88)90688-9","article-title":"Effects of Oky 046, a thromboxane\u2010synthase inhibitor, on renal function in nonazo\u2010temic cirrhotic patients with ascites","volume":"94","author":"GENTILLIN P.","year":"1988","journal-title":"Gastroenterology"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/S0021-9258(17)40652-1","article-title":"Stimulation of human platelet guanylate cyclase by fatty acids","volume":"252","author":"GLASS D.B.","year":"1977","journal-title":"J. Biol. Chem."},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.25.14855"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108718"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.9.3657"},{"key":"e_1_2_5_19_1","first-page":"739","article-title":"Mechanism of vascular smooth muscle relaxation by organic nitrites, nitrites, nitroprusside and nitric oxide: evidence for the involvement of S\u2010nitrosothiols as active intermediates","volume":"218","author":"IGNARRO L.J.","year":"1981","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1836"},{"key":"e_1_2_5_21_1","first-page":"617","volume-title":"Goodman and Gilman's The Pharmacological Basis of Therapeutics","author":"INSEL P.A.","year":"1996"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8052854"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.59.6.612"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7961"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.11122"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V57.5.946.946"},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"24929","DOI":"10.1016\/S0021-9258(19)73985-4","article-title":"Nitric oxide\u2010induced S\u2010nitrosylation of glyceraldehyde\u20103\u2010phosphate dehydrogenase inhibits enzymatic activity and increases endogenous Adp\u2010ribosylation","volume":"267","author":"MOLINA Y.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2580447"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.1638"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.12.1381691"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(57)03489-8"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.23.11543"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118704"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1989.tb12572.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.15.7240"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117185"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb13330.x"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80910-9"},{"key":"e_1_2_5_39_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1182\/blood.V55.4.649.649","article-title":"Inhibition of platelet function by organic nitrate vasodilators","volume":"55","author":"SHAFER A.J.","year":"1980","journal-title":"Blood"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.1.308"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1400"},{"key":"e_1_2_5_42_1","first-page":"163","article-title":"Cyclic Gmp synthesis and function","volume":"39","author":"WALDMAN S.A.","year":"1987","journal-title":"Pharmacol. Rev."}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0701745","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0701745","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0701745","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T00:52:13Z","timestamp":1698454333000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0701745"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1998,4]]}},"alternative-id":["10.1038\/sj.bjp.0701745"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0701745","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4]]},"assertion":[{"value":"2009-02-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}