{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:32:44Z","timestamp":1762144364701},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T00:00:00Z","timestamp":1234396800000},"content-version":"vor","delay-in-days":3970,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The \u03b1<jats:sub>1<\/jats:sub>\u2010adrenoceptor population mediating contractile responses to noradrenaline (NA) in smooth muscles of the bladder neck from rabbit (RBN) has been characterized by use of quantitative receptor pharmacology.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Experiments with several \u2018key\u2019 \u03b1<jats:sub>1<\/jats:sub>\u2010adrenoceptor antagonists of varying subtype selectivities (RS\u201017053, BMY 7378, indoramin, 5\u2010methylurapidil, prazosin, REC 15\/2739, SNAP 5089, terazosin, WB 4101, tamsulosin, (+)\u2010cyclazosin and RS\u2010100329) were conducted. Schild regression analyses yielded affinity (mean p<jats:italic>K<\/jats:italic><jats:sub>b<\/jats:sub>) estimates of 7.1, 6.2, 8.6, 8.6, 8.4, 9.3, 7.0, 7.4, 8.9, 10.0, 7.1 and 9.3, respectively, although deviations from unit Schild regression slope question the robustness of data for RS\u201017053 and SNAP 5089.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The nature of antagonism by these agents and the profile of affinity determinations generated together suggest that a single \u03b1<jats:sub>1<\/jats:sub>\u2010adrenoceptor subtype mediates contractile responses of RBN to NA. Additional studies with phenylephrine indicated also an agonist\u2010independence of this profile. Pharmacologically, this profile was reminiscent of that described as \u2018\u03b1<jats:sub>1L<\/jats:sub>\u2019 \u2010adrenoceptor, which has been shown to mediate contractions of several tissues including lower urinary tract (LUT) tissues of man. Furthermore, a similarity was noticed between the \u2018\u03b1<jats:sub>1L<\/jats:sub>\u2019 \u2010adrenoceptor described here in RBN and the rabbit and human cloned \u03b1<jats:sub>1a<\/jats:sub>\u2010adrenoceptor (based on data from both whole cell radioligand binding at 37\u00b0C and [<jats:sup>3<\/jats:sup>H]\u2010inositol phosphates accumulation assays), characterizations of which have been published elsewhere.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, the RBN appears to provide a predictive pharmacological assay for the study of NA\u2010induced smooth muscle contraction in LUT tissues of man.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>123<\/jats:bold>, 1359\u20131366; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701748\">10.1038\/sj.bjp.0701748<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701748","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:26:01Z","timestamp":1106940361000},"page":"1359-1366","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["\u03b1<sub>1L<\/sub>\u2010Adrenoceptor mediation of smooth muscle contraction in rabbit bladder neck: a model for lower urinary tract tissues of man"],"prefix":"10.1111","volume":"123","author":[{"given":"M.","family":"Shannon Kava","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"David R.","family":"Blue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel L.","family":"Vimont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony P. D. W.","family":"Ford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,12]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"1768P","article-title":"In vitro and in vivo pharmacology of Rs\u2010100975, a novel alpha1A\/1L\u2010adrenoceptor antagonist for benign prostatic hyperplasia (BPH)","volume":"22","author":"BLUE D.R.","year":"1996","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_5_3_1","first-page":"360P","article-title":"Functional pharmacological characteristics of human, rat and rabbit cloned \u03b11A\u2010adrenoceptors expressed in chinese hamster ovary (CHO\u2010K1) cells","volume":"119","author":"DANIELS D.V.","year":"1996","journal-title":"Br. J. 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