{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T20:31:46Z","timestamp":1759091506095},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T00:00:00Z","timestamp":1234396800000},"content-version":"vor","delay-in-days":3970,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>In this study we characterized the CGRP\u2010receptor subtype by Schild\u2010plot analysis using the C\u2010terminal fragment, human\u2010\u03b1CGRP(8\u201337), a putative competitive CGRP<jats:sub>1<\/jats:sub>\u2010receptor selective antagonist. In addition, the effect of rat\u2010\u03b1CGRP was compared with that of homologous peptides rat\u2010\u03b2CGRP, rat\u2010amylin, rat\u2010adrenomedullin and [Cys(Acm)<jats:sup>2,7<\/jats:sup>]\u2010human\u2010\u03b1CGRP, a putative selective CGRP<jats:sub>2<\/jats:sub>\u2010receptor agonist, in the left coronary arteries of 3 months old male and female Sprague Dawley rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Isolated rings from the distal, intramural part of the left anterior descending (LAD) coronary artery in both groups of rats were mounted on a double wire\u2010myograph. The arteries were then stretched to their optimal lumen diameter for active tension development and precontracted with 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> prostaglandin F<jats:sub>2\u03b1<\/jats:sub> (PGF<jats:sub>2\u03b1<\/jats:sub>), after which agonists were added to the organ bath in a cumulative manner.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Rat\u2010\u03b1CGRP induced endothelium\u2010independent relaxations in male and female Sprague\u2010Dawley rats. Rat\u2010\u03b2CGRP concentration\u2010response relations (10<jats:sup>\u221211<\/jats:sup>\u201310<jats:sup>\u22127<\/jats:sup> <jats:sc>m<\/jats:sc>) were similar to those of rat\u2010\u03b1CGRP in either sex. The maximal relaxations induced by rat\u2010amylin and rat\u2010adrenomedullin, both at 10<jats:sup>\u22126<\/jats:sup> <jats:sc>m<\/jats:sc>, were significantly (<jats:italic>P<\/jats:italic>&lt;0.05) lower than those induced by rat\u2010\u03b1\u2010 and rat\u2010\u03b2CGRP. In contrast, the selective CGRP<jats:sub>2<\/jats:sub>\u2010receptor agonist [Cys(Acm)<jats:sup>2,7<\/jats:sup>]\u2010human\u2010\u03b1CGRP failed to induce significant relaxations at the highest concentration used (10<jats:sup>\u22127<\/jats:sup> <jats:sc>m<\/jats:sc>) in the coronary arteries of male and female rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The C\u2010terminal fragment, human\u2010\u03b1CGRP(8\u201337) blocked concentration\u2010dependently (10<jats:sup>\u22127<\/jats:sup>\u201310<jats:sup>\u22126<\/jats:sup> <jats:sc>m<\/jats:sc>) the rat\u2010\u03b1CGRP\u2010induced relaxation in 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc> PGF<jats:sub>2\u03b1<\/jats:sub>\u2010precontracted coronary arteries. The slopes of the regression lines of the Schild\u2010plots for both male and female rats were not significantly (<jats:italic>P<\/jats:italic>&gt;0.05) different from unity and the pA<jats:sub>2<\/jats:sub> values for human\u2010\u03b1CGRP(8\u201337) were 6.93 and 6.98 in arteries from male and female rats, respectively. There was no significant (<jats:italic>P<\/jats:italic>&gt;0.05) difference in estimated p<jats:italic>K<\/jats:italic><jats:sub>B<\/jats:sub> values for human\u2010\u03b1CGRP(8\u201337) between male (6.99\u00b10.10, <jats:italic>n<\/jats:italic>=13) and female (6.95\u00b10.08, <jats:italic>n<\/jats:italic>=13) rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The concentration\u2010response relationships for rat\u2010\u03b1\u2010 and rat\u2010\u03b2CGRP were similar in male and female Sprague Dawley rats. The predominant CGRP receptor subtype in small intramural coronary arteries appeared to belong to the CGRP<jats:sub>1<\/jats:sub>\u2010receptor subtype in both sexes.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>123<\/jats:bold>, 1464\u20131470; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701760\">10.1038\/sj.bjp.0701760<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701760","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:26:01Z","timestamp":1106940361000},"page":"1464-1470","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Characterization of calcitonin gene\u2010related peptide(CGRP) receptors in intramural coronary arteries from male and female Sprague Dawley rats"],"prefix":"10.1111","volume":"123","author":[{"given":"Majid","family":"Sheykhzade","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels C.","family":"Berg Nyborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.19.11325"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/298240a0"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1959.tb00928.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01123504"},{"key":"e_1_2_5_6_1","first-page":"253","article-title":"Calcitonin gene\u2010related peptide in the cardiovascular system: characterization of receptor populations and their (patho)physiological significance","volume":"48","author":"BELL D.","year":"1996","journal-title":"Pharmacol. 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