{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:32:45Z","timestamp":1757701965319},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T00:00:00Z","timestamp":1234224000000},"content-version":"vor","delay-in-days":3968,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of nifedipine on both levcromakalim\u2010induced membrane currents and unitary currents in pig proximal urethra were investigated by use of patch\u2010clamp techniques (conventional whole\u2010cell configuration and cell\u2010attached patches).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Nifedipine had a voltage\u2010dependent inhibitory effect on voltage\u2010dependent Ba<jats:sup>2+<\/jats:sup> currents at \u221250\u2003mV (<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>=30.6\u2003n<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In current\u2010clamp mode, subsequent application of higher concentrations of nifedipine (30\u2003\u03bc<jats:sc>M<\/jats:sc>) caused a significant depolarization even after the membrane potential had been hyperpolarized to approximately \u221282\u2003mV by application of 100\u2003\u03bc<jats:sc>M<\/jats:sc> levcromakalim.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The 100\u2003\u03bc<jats:sc>M<\/jats:sc> levcromakalim\u2010induced inward current (symmetrical 140\u2003m<jats:sc>M<\/jats:sc> K<jats:sup>+<\/jats:sup> conditions, \u221250\u2003mV) was inhibited by additional application of three different types of Ca antagonists (nifedipine, verapamil and diltiazem, all at 100\u2003\u03bc<jats:sc>M<\/jats:sc>). In contrast, Bay K 8644 (1\u2003\u03bc<jats:sc>M<\/jats:sc>) possessed no activating effect on the amplitude of this glibenclamide\u2010sensitive current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>When 100\u2003\u03bc<jats:sc>M<\/jats:sc> nifedipine was included in the pipette solution during conventional whole\u2010cell recording at \u221250\u2003mV, application of levcromakalim (100\u2003\u03bc<jats:sc>M<\/jats:sc>) caused a significant inward membrane current which was suppressed by 5\u2003\u03bc<jats:sc>M<\/jats:sc> glibenclamide. On the other hand, inclusion of 5\u2003\u03bc<jats:sc>M<\/jats:sc> glibenclamide in the pipette solution prevented levcromakalim from inducing an inward membrane current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The levcromakalim\u2010induced K<jats:sup>+<\/jats:sup> channel openings in cell\u2010attached configuration were suppressed by subsequent application of 5\u2003\u03bc<jats:sc>M<\/jats:sc> glibenclamide but not of 100\u2003\u03bc<jats:sc>M<\/jats:sc> nifedipine.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that in pig proximal urethra, nifedipine inhibits the glibenclamide\u2010sensitive 43\u2003pS K<jats:sup>+<\/jats:sup> channel activity mainly through extracellular blocking actions on the K<jats:sup>+<\/jats:sup> channel itself.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>123<\/jats:bold>, 1601\u20131608; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0701777\">10.1038\/sj.bjp.0701777<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0701777","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:27:23Z","timestamp":1106940443000},"page":"1601-1608","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The effects of nifedipine and other calcium antagonists on the glibenclamide\u2010sensitive K<sup>+<\/sup> currents in smooth muscle cells from pig urethra"],"prefix":"10.1111","volume":"123","author":[{"given":"Noriyoshi","family":"Teramoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison F.","family":"Brading","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-198835040-00006"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1992.tb00466.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.109.2.169"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00584106"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374735"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(89)90580-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.5.C1119"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90004-4"},{"key":"e_1_2_6_10_1","first-page":"H1737","article-title":"Inhibition of transient outward K+ current by DHP Ca2+ antagonists and agonists in rabbit cardiac myocytes","volume":"260","author":"Gotoh Y.","year":"1991","journal-title":"Am. 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