{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T22:09:35Z","timestamp":1782166175911,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":3931,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We evaluated the effect of the nitric oxide (NO) donor CysNO (S\u2010nitroso\u2010<jats:sc>L<\/jats:sc>\u2010cysteine) and endogenous NO upon spontaneous contractility in non\u2010pregnant cynomolgus monkeys. We also assessed the role of intracellular guanosine 3\u2032,5\u2032\u2010cyclic monophosphate ([cyclic GMP]<jats:sub>i<\/jats:sub>) as a second messenger for NO in monkey uterine smooth muscle.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>CysNO reduced spontaneous contractility by 84% (<jats:italic>P<\/jats:italic>&lt;0.05) at maximal concentrations, and significantly elevated [cyclic GMP]<jats:sub>i<\/jats:sub> (<jats:italic>P<\/jats:italic>&lt;0.05). However, increases in [cyclic GMP]<jats:sub>i<\/jats:sub> were not required for CysNO\u2010induced relaxations; CysNO inhibited contractile activity despite the complete inhibition of guanylyl cyclase by methylene blue or LY83,583.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Analogues of cyclic GMP had no significant effect upon spontaneous contractile activity. <jats:sc>L<\/jats:sc>\u2010arginine produced a 62% reduction in spontaneous activity (<jats:italic>P<\/jats:italic>&lt;0.05) while <jats:sc>D<\/jats:sc>\u2010arginine had no effect. The competitive nitric oxide synthase (NOS) inhibitor N<jats:sup>\u03c9<\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine (<jats:sc>L<\/jats:sc>\u2010NOARG) not only blocked <jats:sc>L<\/jats:sc>\u2010arginine\u2010induced relaxations, but also significantly increased spontaneous contractile activity when added alone (<jats:italic>P<\/jats:italic>&lt;0.05); the inactive <jats:sc>D<\/jats:sc>\u2010enantiomer of NOARG had no such effect.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>While both endogenous NO and the NO donor CysNO relax monkey myometrium, this effect is not causally related to CysNO\u2010induced elevations in [cyclic GMP]<jats:sub>i<\/jats:sub>. The failure of cyclic GMP analogues to alter monkey uterine smooth muscle tension also argues against a role for [cyclic GMP]<jats:sub>i<\/jats:sub> in the regulation of uterine contractility. Not only do these findings argue for the existence of a functionally\u2010relevant NOS in the monkey uterus, but increases in contractile activity seen in the presence of NOS inhibitors suggest a role for NO in the moment\u2010to\u2010moment regulation of contractile activity in this organ.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1038\/sj.bjp.0701799","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:29:55Z","timestamp":1106940595000},"page":"63-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Nitric oxide regulation of monkey myometrial contractility"],"prefix":"10.1111","volume":"124","author":[{"given":"Karri A","family":"Kuenzli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain L O","family":"Buxton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.29.1.320"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/368850a0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(95)90500-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.7.6.7682524"},{"key":"e_1_2_6_6_1","first-page":"422","article-title":"Lack of correlation between cyclic GMP elevation and relaxation of nonvascular smooth muscle by nitroglycerin, nitroprusside, hydroxylamine and sodium azide","volume":"225","author":"Diamond J.","year":"1983","journal-title":"J. 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