{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:03:00Z","timestamp":1790420580560,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":3931,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The ionotropic purinoceptors in isolated Deiters' cells of guinea\u2010pig cochlea were characterized by use of the whole\u2010cell variant of the patch\u2010clamp technique.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Extracellular application of adenosine 5\u2032\u2010triphosphate (ATP) induced a dose\u2010dependent inward current when the cells were voltage\u2010clamped at \u221280 mV. The ATP\u2010induced current showed desensitization and had a reversal potential around \u22124 mV.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Increasing intracellular free Ca<jats:sup>2+<\/jats:sup> by decreasing the concentration of EGTA in the pipette solution reduced the amplitude of the ATP\u2010gated current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The order of agonist potency was: 2\u2010methylthioATP (2\u2010meSATP)&gt;ATP&gt;benzoylbenzoyl\u2010ATP (BzATP)&gt;\u03b1,\u03b2\u2010methyleneATP (\u03b1,\u03b2,meATP&gt;adenosine 5\u2032\u2010diphosphate (ADP)&gt;uridine 5\u2032\u2010triphosphate (UTP)&gt;adenosine 5\u2032\u2010monophosphate (AMP)=adenosine (Ad).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pretreatment with forskolin (10 \u03bc<jats:sc>M<\/jats:sc>), 8\u2010bromoadenosine\u20103\u2032,5\u2032\u2010cyclophosphate (8\u2010Br\u2010cyclic AMP, 1 m<jats:sc>M<\/jats:sc>), 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX, 1 m<jats:sc>M<\/jats:sc>) or phorbol\u201012\u2010myristate\u201013\u2010acetate (PMA, 1 \u03bc<jats:sc>M<\/jats:sc>) reversibly reduced the ATP\u2010induced peak current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results are consistent with molecular biological data which indicate that P2X<jats:sub>2<\/jats:sub> purinoceptors are present in Deiters' cells. In addition, the reduction of the ATP\u2010gated current by activators of protein kinase A and protein kinase C indicates that these P2X<jats:sub>2<\/jats:sub> purinoceptors can be functionally modulated by receptor phosphorylation.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1038\/sj.bjp.0701848","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:21:10Z","timestamp":1106940070000},"page":"337-344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["P2X receptors in cochlear Deiters' cells"],"prefix":"10.1111","volume":"124","author":[{"given":"Chu","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P","family":"Bobbin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018203"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(92)90032-2"},{"key":"e_1_2_6_4_1","first-page":"29","volume-title":"Hair Cells and Hearing Aids","author":"Bobbin R.P.","year":"1996"},{"key":"e_1_2_6_5_1","volume-title":"Otoacoustic Emmissions","author":"Bobbin R.P.","year":"1997"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/371519a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00128-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00333.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(97)00040-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb37657.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/377428a0"},{"key":"e_1_2_6_12_1","first-page":"1782","article-title":"Differences in effects of purinergic antagonists on P2x receptor in the cochlea","volume":"23","author":"Chen C.","year":"1997","journal-title":"Soc. 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