{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T03:41:58Z","timestamp":1788838918134,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T00:00:00Z","timestamp":1234224000000},"content-version":"vor","delay-in-days":3877,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,7]]},"abstract":"\n \n \n Since both histamine and 5\u2010hydroxytryptamine (5\u2010HT) can be released by murine mast cells, we investigated the possible role of these autacoids on airway hyperresponsiveness (AHR), eosinophil infiltration and serum\u2010IgE levels in a murine model of allergic asthma.<\/jats:p>\n <\/jats:list-item>\n \n \n Ovalbumin\u2010sensitized mice were exposed to either ovalbumin (2 mg ml\n \u22121<\/jats:sup>\n ) or saline aerosols on 8 consecutive days. Starting one day before the challenge, animals were injected i.p. twice a day with a 5\u2010HT\u2010type 1 (5\u2010HT\n 1<\/jats:sub>\n ) or type 2 (5\u2010HT\n 2<\/jats:sub>\n ) receptor antagonist (methiotepine, 1.25 or 2.0 mg kg\n \u22121<\/jats:sup>\n and ketanserin, 12 mg kg\n \u22121<\/jats:sup>\n , respectively) or a histamine\u2010type 1 (H\n 1<\/jats:sub>\n ) or type 2 (H\n 2<\/jats:sub>\n ) receptor antagonist (mepyramine, 12 or 20 mg kg\n \u22121<\/jats:sup>\n and cimetidine, 10 or 25 mg kg\n \u22121<\/jats:sup>\n , respectively). Furthermore, animals were injected with a combination of cimetidine and ketanserin or with an \u03b1\u2010adrenoceptor antagonist (phentolamine, 5 mg kg\n \u22121<\/jats:sup>\n ).\n <\/jats:p>\n <\/jats:list-item>\n \n \n In vehicle\u2010treated ovalbumin\u2010challenged animals airway responsiveness to intravenous injections of methacholine\n in vivo<\/jats:italic>\n was significantly (9 fold increase,\n P<\/jats:italic>\n <0.01) increased when compared to vehicle\u2010treated saline\u2010challenged animals. Furthermore, ovalbumin challenge of vehicle\u2010treated animals induced a significant increase in both eosinophil numbers in bronchoalveolar lavage (BAL) fluid (0\u00b10, vehicle\/saline and 15.0\u00b15.9\u00d710\n 4<\/jats:sup>\n cells vehicle\/ovalbumin,\n P<\/jats:italic>\n <0.05) and ovalbumin\u2010specific IgE levels in serum (157\u00b169 and 617\u00b1171 units ml\n \u22121<\/jats:sup>\n , respectively,\n P<\/jats:italic>\n <0.05) compared to saline\u2010challenged mice. Virtually no eosinophils could be detected in saline\u2010challenged animals after all different treatments.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Treatment with ketanserin or cimetidine resulted in a partial but significant decrease of the ovalbumin\u2010induced AHR compared to ovalbumin\u2010challenged controls (\n P<\/jats:italic>\n <0.05) and reduced eosinophil infiltration after ovalbumin challenge by 60% and 58%, respectively. The combination of cimetidine and ketanserin almost completely abolished AHR whereas eosinophilia was decreased by 49%. No effects of these antagonists were observed on IL\u201016 levels in BAL fluid or on serum antigen\u2010specific IgE levels. Treatment with either the H\n 1<\/jats:sub>\n \u2010receptor, the 5\u2010HT\n 1<\/jats:sub>\n \u2010receptor or the \u03b1\u2010adrenoceptor antagonist, did not decrease the observed ovalbumin\u2010induced airway responsiveness or eosinophilia in vehicle\u2010treated animals. Higher doses of either methiotepine (2.0 mg kg\n \u22121<\/jats:sup>\n ) or mepyramine (20 mg kg\n \u22121<\/jats:sup>\n ) did decrease ovalbumin\u2010induced eosinophil infiltration (by 67%,\n P<\/jats:italic>\n <0.05 and 73%, respectively), whereas no effects of these antagonists were observed on ovalbumin\u2010specific IgE levels in serum.\n <\/jats:p>\n <\/jats:list-item>\n \n From these data it can be concluded that both histamine and 5\u2010HT play a role in antigen\u2010induced AHR and eosinophilia in the mouse.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>","DOI":"10.1038\/sj.bjp.0701901","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T14:24:32Z","timestamp":1106922272000},"page":"857-864","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Modulation of airway hyperresponsiveness and eosinophilia by selective histamine and 5\u2010HT receptor antagonists in a mouse model of allergic asthma"],"prefix":"10.1111","volume":"124","author":[{"given":"J J.","family":"De Bie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P A J.","family":"Henricks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W W.","family":"Cruikshank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Hofman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E H.","family":"Jonker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F P.","family":"Nijkamp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J M.","family":"Van Oosterhout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.4049\/jimmunol.142.9.3171","article-title":"A new interpretation of the involvement of serotonin in delayed\u2010type hypersensitivity","volume":"142","author":"Ameisen J.\u2010C.","year":"1989","journal-title":"J. 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