{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:49:40Z","timestamp":1761540580283},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":3808,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The mechanisms involved in the protective effect of amylin (administered into the brain ventricle, i.c.v.) on gastric ulcers induced by the oral administration of ethanol 50% (EtOH, 1\u2003ml\/rat) or indomethacin (indomethacin, 20\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, at a dosing volume of 5\u2003ml) were investigated in rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The possible involvement of endogenous nitric oxide (NO) in the beneficial effect of amylin against EtOH\u2010induced ulcers was examined. The inhibitor of NO\u2010synthesis, N<jats:sup>G<\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine methyl ester (<jats:sc>L<\/jats:sc>\u2010NAME, 70\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, s.c.) was injected 30\u2003min before amylin (2.2\u2003\u03bcg\/rat, i.c.v.) followed by EtOH after a further 30\u2003min. Rats were sacrified 1\u2003h after EtOH. <jats:sc>L<\/jats:sc>\u2010NAME completely removed the protective effect of amylin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The interaction between amylin and gastric nonprotein sulfhydryl groups was studied. The rats were treated with N\u2010ethyl\u2010maleimide (NEM, 25\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, s.c.) 30\u2003min before amylin (2.2\u2003\u03bcg\/rat, i.c.v.) followed by EtOH 30\u2003min after or by indomethacin 5\u2003min after amylin. Rats were sacrified 1\u2003h or 6\u2003h respectively after EtOH or indomethacin. NEM counteracted the protective effect of amylin against EtOH\u2010induced ulcers but not against those provoked by indomethacin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>To determine whether amylin was able to promote ulcer healing, the peptide was injected 5\u2003min after EtOH or 1\u2003h after indomethacin. In the case of EtOH, the beneficial effect of amylin was lost whereas it was still effective on indomethacin\u2010induced ulcers.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results indicate that: the mechanisms involved in the antiulcer effects of amylin are different in these two types of gastric lesions probably because of the different etiopathology of various types of ulcers. Endogenous NO and nonprotein sulfhydryl groups are involved in the mucosal protective effects of amylin on EtOH and not on indomethacin\u2010induced ulcers. Furthermore the effectiveness of amylin against indomethacin\u2010induced lesions when administered after the ulcerogenic process has started suggests that amylin is involved not only in the protection but also in the healing mechanisms in this type of ulcer.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>125<\/jats:bold>, 23\u201328; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702029\">10.1038\/sj.bjp.0702029<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702029","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:36:52Z","timestamp":1106941012000},"page":"23-28","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Investigation on the mechanisms involved in the central protective effect of amylin on gastric ulcers in rats"],"prefix":"10.1111","volume":"125","author":[{"given":"F","family":"Guidobono","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Pagani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Ticozzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Sibilia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Netti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"113","article-title":"Pharmacological research on gefarnate, a new synthetic isoprenoid with antiulceration activity","volume":"147","author":"Adami R.","year":"1964","journal-title":"Int. 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