{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:48:33Z","timestamp":1791179313651,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T00:00:00Z","timestamp":1234224000000},"content-version":"vor","delay-in-days":3754,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Adenine nucleotides have been shown to stimulate insulin secretion by acting on P2 receptors of the P2Y type. Since there have been some discrepancies in the insulin response of different analogues of ATP and ADP, we investigated whether two different types of P2 receptors exist on pancreatic B cells. The effects of \u03b1,\u03b2\u2010methylene ATP, which is more specific for the P2X subtype, were studied <jats:italic>in vitro<\/jats:italic> in pancreatic islets and isolated perfused pancreas from rats, in comparison with the potent P2Y receptor agonist ADP\u03b2S.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In isolated islets, incubated with a slightly stimulating glucose concentration (8.3\u2003m<jats:sc>m<\/jats:sc>), \u03b1,\u03b2\u2010me ATP (200\u2003\u03bc<jats:sc>m<\/jats:sc>) and ADP\u03b2S (50\u2003\u03bc<jats:sc>m<\/jats:sc>) similarly stimulated insulin secretion; by contrast, under a non stimulating glucose concentration (3\u2003m<jats:sc>m<\/jats:sc>), \u03b1,\u03b2\u2010me ATP was still effective whereas ADP\u03b2S was not. In the same way, in islets perifused with 3\u2003m<jats:sc>m<\/jats:sc> glucose, \u03b1,\u03b2\u2010me ATP but not ADP\u03b2S induced a partial but significant reduction in the peak <jats:sup>86<\/jats:sup>Rb efflux induced by the ATP\u2010dependent potassium channel opener diazoxide.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the isolated pancreas, perfused with a non stimulating glucose concentration (4.2\u2003m<jats:sc>m<\/jats:sc>), ADP\u03b2S and \u03b1,\u03b2\u2010me ATP (5\u201350\u2003\u03bc<jats:sc>m<\/jats:sc>), administered for 10\u2003min, induced an immediate, transient and concentration\u2010dependent increase in the insulin secretion; their relative potency was not significantly different. In contrast, with a slightly stimulating glucose concentration (8.3\u2003m<jats:sc>m<\/jats:sc>), ADP\u03b2S was previously shown to be 100 fold more potent than \u03b1,\u03b2\u2010me ATP. Furthermore, at 4.2\u2003m<jats:sc>m<\/jats:sc> glucose a second administration of \u03b1,\u03b2\u2010me ATP was ineffective. In the same way, ADP\u03b2S was also able to desensitize its own insulin response. At 3\u2003m<jats:sc>m<\/jats:sc> glucose, \u03b1,\u03b2\u2010me ATP as well as ADP\u03b2S (50\u2003\u03bc<jats:sc>m<\/jats:sc>) induced a transient stimulation of insulin secretion and down regulated the action of each other.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results give evidence that pancreatic B cells, in addition to P2Y receptors, which potentiate glucose\u2010induced insulin secretion, are provided with P2X receptors, which transiently stimulate insulin release at low non\u2010stimulating glucose concentration and slightly affect the potassium conductance of the membrane.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>125<\/jats:bold>, 1368\u20131374; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702214\">10.1038\/sj.bjp.0702214<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702214","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:52:52Z","timestamp":1106941972000},"page":"1368-1374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Evidence for two different types of P2 receptors stimulating insulin secretion from pancreatic B cell"],"prefix":"10.1111","volume":"125","author":[{"given":"P","family":"Petit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Hillaire\u2010Buys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Manteghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Debrus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Chapal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M M","family":"Loubati\u00e8res\u2010Mariani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1987.tb11276.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12223.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1989.tb14631.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(88)90182-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(85)90001-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1981.tb16778.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(81)90522-7"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(83)90081-X"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(89)90018-9"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1987.tb11322.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/271271a0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(82)90036-3"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-25-10-1375"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.1994.tb00788.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701826"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(91)90494-B"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb14383.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(82)90330-2"},{"key":"e_1_2_6_20_1","first-page":"30","article-title":"P1\u2010 and P2\u2010purinoceptor subtypes: an update","volume":"303","author":"Kennedy C.","year":"1990","journal-title":"Arch. 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