{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T13:21:10Z","timestamp":1698585670966},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3681,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The adrenoceptor in a mouse pineal gland tumour cell line (PGT\u2010\u03b2) was identified and characterized using pharmacological and physiological approaches.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Adrenaline and noradrenaline, adrenoceptor agonists, stimulated cyclic AMP generation in a concentration\u2010dependent manner, but had no effect on inositol 1,4,5\u2010trisphosphate production. Adrenaline was a more potent activator of cyclic AMP generation than noradrenaline, with half maximal\u2010effective concentrations (EC<jats:sub>50<\/jats:sub>) seen at 175\u00b122\u2003n<jats:sc>M<\/jats:sc> and 18\u00b12\u2003\u03bc<jats:sc>M<\/jats:sc> for adrenaline and noradrenaline, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The addition of forskolin synergistically stimulated the adrenaline\u2010mediated cyclic AMP generation in a concentration\u2010dependent manner.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The pA<jats:sub>2<\/jats:sub> value for the specific \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptor antagonist ICI\u2010118,551 (8.7\u00b10.4) as an antagonist of the adrenaline\u2010stimulated cyclic AMP generation were \u223c3 units higher than the value for the \u03b2<jats:sub>I<\/jats:sub>\u2010adrenoceptor antagonist atenolol (5.6\u00b10.3).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Treatment of the cells with adrenaline and forskolin evoked a \u223c3 fold increase in the activity of serotonin N\u2010acetyltransferase with the peak occurring 6\u2003h after stimulation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest the presence of \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptors in mouse pineal cells and a functional relationship between the adenylyl cyclase system and the regulation of N\u2010acetyltransferase expression.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>126<\/jats:bold>, 399\u2013406; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702248\">10.1038\/sj.bjp.0702248<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702248","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:57:44Z","timestamp":1106942264000},"page":"399-406","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Pharmacological characterization of \u03b2<sub>2<\/sub>\u2010adrenoceptor in PGT\u2010\u03b2 mouse pineal gland tumour cells"],"prefix":"10.1111","volume":"126","author":[{"given":"Byung\u2010Chang","family":"Suh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee\u2010Don","family":"Chae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joo\u2010Ho","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyong\u2010Tai","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(93)90015-R"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.6979"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.181.4096.273-a"},{"key":"e_1_2_6_5_1","first-page":"1094","article-title":"Structural and conformational features determining selective signal transduction in the \u03b23\u2010adrenergic receptor","volume":"44","author":"BLIN N.","year":"1993","journal-title":"Mol. 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