{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:28:47Z","timestamp":1760297327727},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3681,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The inhibitory effects of nitric oxide (NO) on N\u2010methyl\u2010<jats:sc>D<\/jats:sc>\u2010aspartate (NMDA) receptor function have been proposed to be mediated <jats:italic>via<\/jats:italic> the interaction of this gas with a redox\u2010sensitive thiol moiety on the receptor. Here, we evaluated this suggested mechanism by examining the actions of various NO donors on native neuronal receptors as well as in wild\u2010type and cysteine\u2010mutated recombinant NMDA receptors expressed in Chinese hamster ovary (CHO) cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The NO donor N\u2010ethyl\u20102\u2010(1\u2010ethyl\u20102\u2010hydroxy\u20102\u2010nitrosohydraxino)ethanamine (NOC\u201012; 100\u2003\u03bc<jats:sc>M<\/jats:sc>) produced a rapid and readily reversible inhibition of whole\u2010cell currents induced by NMDA (30\u2003\u03bc<jats:sc>M<\/jats:sc>) in cultured cortical neurons. The inhibition was apparent at all holding potentials, though a more pronounced block was observed at negative voltages. The effects of NOC\u201012 disappeared when the donor was allowed to expire. A similar receptor block was observed with another NO\u2010releasing agent, S\u2010nitroso\u2010N\u2010acetylpenicillamine (SNAP; 1\u2003m<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The blocking effects of NO released by SNAP, 3\u2010morpholinosydnonimine (SIN\u20101; 1\u2003m<jats:sc>M<\/jats:sc>), and 3\u2010[2\u2010hydroxy\u20101\u2010(1\u2010methylethyl)\u20102\u2010nitrosohydrazino]\u20101\u2010propanamine (NOC\u20105; 100\u2003\u03bc<jats:sc>M<\/jats:sc>) on currents mediated by recombinant NR1\/NR2B receptors were virtually indistinguishable from those observed on native receptors. Furthermore, mutating cysteines 744 and 798 of NR1, which constitute the principal redox modulatory site of the NR1\/NR2B receptor configuration, did not affect the inhibition produced by NO.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The NR2A subunit may contribute its own redox\u2010sensitive site. However, the effects of NO on NR1\/NR2A receptors were very similar to those seen for all other receptor configurations evaluated. Hence, we conclude that NO does not exert its inhibition of NMDA\u2010induced responses <jats:italic>via<\/jats:italic> a modification of any of the previously described redox\u2010sensitive sites on the receptor.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>126<\/jats:bold>, 296\u2013300; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702295\">10.1038\/sj.bjp.0702295<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702295","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:54:15Z","timestamp":1106942055000},"page":"296-300","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Lack of interaction between nitric oxide and the redox modulatory site of the NMDA receptor"],"prefix":"10.1111","volume":"126","author":[{"given":"Elias","family":"Aizenman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William K","family":"Potthoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"95","volume-title":"Direct and allosteric control of glutamate receptors","author":"AIZENMAN E.","year":"1994"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90447-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90310-3"},{"key":"e_1_2_7_5_1","first-page":"21505","article-title":"Subunit\u2010specific interactions of cyanide with the N\u2010methyl\u2010D\u2010aspartate receptor","volume":"273","author":"ARDEN S.R.","year":"1998","journal-title":"Proc. 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