{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:22:50Z","timestamp":1792113770742,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3622,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The receptors which mediate the effects of calcitonin gene\u2010related peptide (CGRP), amylin and adrenomedullin on the guinea\u2010pig vas deferens have been investigated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>All three peptides cause concentration dependant inhibitions of the electrically stimulated twitch response (pD<jats:sub>2<\/jats:sub>s for CGRP, amylin and adrenomedullin of 7.90\u00b10.11, 7.70\u00b10.19 and 7.25\u00b10.10 respectively).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>CGRP<jats:sub>8\u201337<\/jats:sub> (1\u2003\u03bc<jats:sc>M<\/jats:sc>) and AC187 (10\u2003\u03bc<jats:sc>M<\/jats:sc>) showed little antagonist activity against adrenomedullin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Adrenomedullin<jats:sub>22\u201352<\/jats:sub> by itself inhibited the electrically stimulated contractions of the vas deferens and also antagonized the responses to CGRP, amylin and adrenomedullin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>[<jats:sup>125<\/jats:sup>I]\u2010adrenomedullin labelled a single population of binding sites in vas deferens membranes with a pIC<jats:sub>50<\/jats:sub> of 8.91 and a capacity of 643\u2003\u2003fmol\u2003\u2003mg<jats:sup>\u22121<\/jats:sup>. Its selectivity profile was adrenomedullin&gt; AC187&gt;CGRP=amylin. It was clearly distinct from a site labelled by [<jats:sup>125<\/jats:sup>I]\u2010CGRP (pIC<jats:sub>50<\/jats:sub>=8.73, capacity=114\u2003\u2003fmol\u2003\u2003mg<jats:sup>\u22121<\/jats:sup>, selectivity CGRP&gt;amylin=AC187&gt;adrenomedullin). [<jats:sup>125<\/jats:sup>I]\u2010amylin bound to two sites with a total capacity of 882 fmol mg<jats:sup>\u22121<\/jats:sup>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Although CGRP has been shown to act at a CGRP<jats:sub>2<\/jats:sub> receptor on the vas deferens with low sensitivity to CGRP<jats:sub>8\u201337<\/jats:sub>, this antagonist displaced [<jats:sup>125<\/jats:sup>I]\u2010CGRP with high affinity from vas deferens membranes. This affinity was unaltered by increasing the temperature from 4\u00b0C to 25\u00b0C, suggesting the anomalous behaviour of CGRP<jats:sub>8\u201337<\/jats:sub> is not due to temperature differences between binding and functional assays.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>126<\/jats:bold>, 1276\u20131282; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702437\">10.1038\/sj.bjp.0702437<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702437","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:42:21Z","timestamp":1106941341000},"page":"1276-1282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Characterization of receptors for calcitonin gene\u2010related peptide and adrenomedullin on the guinea\u2010pig vas deferens"],"prefix":"10.1111","volume":"126","author":[{"given":"David R","family":"Poyner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian M","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A Elaine","family":"Tomlinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan G","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.65031131.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.19.11325"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb14991.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.130.2.906"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.5.2351"},{"key":"e_1_2_7_7_1","first-page":"R234","article-title":"Adrenomedullin\u2010(22\u201352) antagonises the vasodilator responses to calcitonin gene\u2010related peptide but not adrenomedullin in the cat","volume":"272","author":"CHAMPION H.C.","year":"1997","journal-title":"Am. 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