{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T13:08:20Z","timestamp":1754485700133},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3561,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We have studied the effects of \u03bc and \u03b4 opioids on intestinal function (permeability, PER; gastrointestinal transit, GIT), and their antagonism after the intracerebroventricular (i.c.v.) administration of specific antibodies (ABs) or antisense oligodeoxynucleotides (ODN) to \u03bc\u2010receptors (OR). Central versus peripheral site\/s of action of subcutaneous (s.c.) \u03bc\u2010opioids, were also assessed.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Male Swiss CD\u20101 mice were used. GIT was measured with charcoal and PER by the passage of <jats:sup>51<\/jats:sup>Cr\u2010EDTA from blood to lumen.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Morphine and fentanyl (i.c.v. and s.c.) inhibited GIT and PER in a dose\u2010related manner; they were more potent by i.c.v. route, both on GIT and PER (70 and 17 times for morphine and fentanyl). They also had a greater effect on GIT than PER (4.3 and 1.6 times). DPDPE had a lower potency than \u03bc\u2010agonists in all experiments, and no dose\u2010response could be obtained after s.c. administration on GIT.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Pretreatment with i.c.v. ABs (24\u2003h) or antisense ODN (5 days), decreased the effects (GIT and PER) of i.c.v. morphine and fentanyl, while those of DPDPE remained unchanged. The ABs did not alter the peripheral effects of \u03bc\u2010opioids.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results show that (i.c.v. or s.c.) \u03bc opioids produce dose\u2010related inhibitions of PER and GIT, being more potent by the i.c.v. route. Delta\u2010opioids had a greater effect on PER than GIT, while the opposite occurred for \u03bc\u2010agonists. Pretreatment with ABs or ODN to \u03bc\u2010OR, blocked the central effects of \u03bc (but not \u03b4) agonists on GIT and PER.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>127<\/jats:bold>, 397\u2013404; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702570\">10.1038\/sj.bjp.0702570<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702570","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:03:49Z","timestamp":1106942629000},"page":"397-404","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Antibodies and antisense oligodeoxynucleotides to \u03bc\u2010opioid receptors, selectively block the effects of \u03bc\u2010opioid agonists on intestinal transit and permeability in mice"],"prefix":"10.1111","volume":"127","author":[{"given":"Olga","family":"Pol","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Llu\u00eds","family":"Valle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"S\u00e1nchez\u2010Bl\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Garz\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita M","family":"Puig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"491","article-title":"Characterization of antinociception to opioid receptor selective agonists after antisense oligodeoxynucleotide\u2010mediated \u2018knock\u2010down\u2019 of opioid receptors in vivo","volume":"277","author":"BILSKY E.J.","year":"1996","journal-title":"J. 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