{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:51:15Z","timestamp":1761580275224},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3500,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Nitric oxide synthase\u2010containing nerve fibres are abundant within taenia of the guinea\u2010pig caecum, but there is little previous evidence supporting a direct role for nitric oxide (NO) in responses to enteric inhibitory nerve stimulation. In this study we have attempted to identify an NO\u2010dependent component of inhibitory transmission in isolated taenia coli.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Isometric tension was recorded in the presence of atropine and guanethidine (both 1\u2003\u03bc<jats:sc>M<\/jats:sc>). Tone was raised with histamine (1\u2003\u03bc<jats:sc>M<\/jats:sc>), and intrinsic inhibitory neurons stimulated using either a nicotinic agonist (1,1\u2010dimethyl\u20104\u2010phenylpiperazinium iodide; DMPP) or electrical field stimulation (EFS).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>DMPP (1\u2013100\u2003\u03bc<jats:sc>M<\/jats:sc>) produced concentration\u2010dependent biphasic relaxations, comprising an initial peak relaxation followed by a sustained relaxation. Responses to DMPP were antagonized by tetrodotoxin (1\u2003\u03bc<jats:sc>M<\/jats:sc>) or apamin (0.3\u2003\u03bc<jats:sc>M<\/jats:sc>) and abolished by hexamethonium (300\u2003\u03bc<jats:sc>M<\/jats:sc>). <jats:sc>L<\/jats:sc>\u2010nitro\u2010arginine (<jats:sc>L<\/jats:sc>\u2010NOARG; 100\u2003\u03bc<jats:sc>M<\/jats:sc>) and oxyhaemoglobin (2%) both significantly reduced sustained relaxations produced by DMPP.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>EFS (5\u2003Hz, 30\u2003s) also produced biphasic relaxations. Both <jats:sc>L<\/jats:sc>\u2010NOARG and an inhibitor of soluble guanylate cyclase (ODQ, 1\u201310\u2003\u03bc<jats:sc>M<\/jats:sc>) reduced the sustained component of EFS responses.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Two NO donors, sodium nitroprusside (SNP) and diethylenetriamine\u2010nitric oxide adduct (DENO), produced concentration\u2010dependent relaxations. Responses to SNP and DENO were antagonized by ODQ (1\u2003\u03bc<jats:sc>M<\/jats:sc>) and by apamin (0.3\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that NO contributes directly to a component of inhibitory transmission in guinea\u2010pig taenia coli. The actions of NO appear to be mediated <jats:italic>via<\/jats:italic> cyclic GMP synthesis, and may involve activation of small conductance calcium activated K<jats:sup>+<\/jats:sup> channels. A role for NO is most evident during sustained relaxations evoked by longer stimulus trains or chemical stimulation of intrinsic neurons.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>127<\/jats:bold>, 1495\u20131501; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702674\">10.1038\/sj.bjp.0702674<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702674","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:17:07Z","timestamp":1106943427000},"page":"1495-1501","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evidence that nitric oxide acts as an inhibitory neurotransmitter supplying taenia from the guinea\u2010pig caecum"],"prefix":"10.1111","volume":"127","author":[{"given":"C W R","family":"Shuttleworth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K M","family":"Sweeney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K M","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1966.sp007976"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014250"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020719"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(64)90003-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1970.tb10646.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00633202"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb17124.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701487"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00303090"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00381887"},{"key":"e_1_2_7_12_1","first-page":"184","article-title":"Potent and selective inhibition of nitric oxide\u2010sensitive guanylyl cyclase by 1H\u2010[1,2,4]oxadiazolo[4,3\u2010a]quinoxalin\u20101\u2010one","volume":"48","author":"GARTHWAITE J.","year":"1995","journal-title":"Mol. 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