{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T12:42:54Z","timestamp":1782736974666,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3500,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The cardioprotective properties of inhibition of poly (ADP\u2010ribose) synthetase (PARS) were investigated in the isolated perfused heart of the rat. Hearts were perfused in the Langendorff mode and subjected to 23\u2003min total global ischaemia and reperfused for 60\u2003min.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Left ventricular function was assessed by means of an intra\u2010ventricular balloon. High energy phosphates were measured by <jats:sup>31<\/jats:sup>P\u2010NMR spectroscopy. Intracellular levels of NAD were measured by capillary electrophoresis of perchloric acid extracts of hearts at the end of reperfusion.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Reperfusion in the presence of the PARS inhibitor 1,5 didroxyisoquinoline (ISO, 100\u2003\u03bc<jats:sc>M<\/jats:sc>) attenuated the mechanical dysfunction observed following 1\u2003h of reperfusion; 27\u00b113 and 65\u00b18% recovery of preischaemic rate pressure product for control and 100\u2003\u03bc<jats:sc>M<\/jats:sc> ISO, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>This cardioprotection was accompanied by a preservation of intracellular high\u2010energy phosphates during reperfusion; 38\u00b12 vs 58\u00b14% (<jats:italic>P<\/jats:italic>&lt;0.05) of preischaemic levels of phosphocreatine (PCr) for control and 100\u2003\u03bc<jats:sc>M<\/jats:sc> ISO respectively and 23\u00b11 vs 31\u00b13% (<jats:italic>P<\/jats:italic>&lt;0.05) of preischaemic levels of ATP for control and 100\u2003\u03bc<jats:sc>M<\/jats:sc> ISO respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cellular levels of NAD were higher in ISO treated hearts at the end of reperfusion; 2.56\u00b10.45 vs 4.76\u00b11.12\u2003\u03bcmoles\u2003g<jats:sup>\u22121<\/jats:sup> dry weight (<jats:italic>P<\/jats:italic>&lt;0.05) for control and ISO treated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results demonstrate that the cardioprotection afforded by inhibition of PARS activity with ISO is accompanied by a preservation of high\u2010energy phosphates and cellular NAD levels and suggest that the mechanism responsible for this cardioprotection may involve prevention of intracellular ATP depletion.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>127<\/jats:bold>, 1518\u20131524; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702705\">10.1038\/sj.bjp.0702705<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702705","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:17:07Z","timestamp":1106943427000},"page":"1518-1524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["An inhibitor of poly (ADP\u2010ribose) synthetase activity reduces contractile dysfunction and preserves high energy phosphate levels during reperfusion of the ischaemic rat heart"],"prefix":"10.1111","volume":"127","author":[{"given":"John C","family":"Docherty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bozena","family":"Kuzio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn A","family":"Silvester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Bowes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Thiemermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.74.6.1424"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/3576299"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113621"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00221-1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702009"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.71.6.1334"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(86)90028-5"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1997.0496"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-2584-4_169"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0864"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.67.6.1453"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01906863"},{"key":"e_1_2_7_14_1","first-page":"187","article-title":"Relation between high energy phosphate and lethal injury in myocardial ischemia in the dog","volume":"92","author":"JENNINGS R.B.","year":"1978","journal-title":"Am. 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