{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T19:34:37Z","timestamp":1773430477322,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":3469,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p><jats:italic>Systemic<\/jats:italic> application of U37883A, a blocker of ATP sensitive potassium (K<jats:sub>ATP<\/jats:sub>) channels, elicits diuresis and natriuresis without significantly altering urinary potassium excretion.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>To elucidate tubular sites of action upstream to the distal nephron, micropuncture experiments were performed in nephrons with superficial glomeruli of anaesthetized Munich\u2010Wistar\u2010Fr\u00f6mter rats during <jats:italic>systemic<\/jats:italic> application of U37883A (1, 5 or 15\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup> i.v.).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The observed eukaliuric diuresis and natriuresis in response to U37883A at 15\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup> was accompanied by an increase in early distal tubular flow rate (V<jats:sub>ED<\/jats:sub>) from 10\u201318\u2003nl\u2003min<jats:sup>\u22121<\/jats:sup> reflecting a reduction in fractional reabsorption of fluid up to this site (FR\u2010fluid) of 13%. The latter proposed an effect on water\u2010permeable segments such as the proximal tubule which could fully account for the observed reduction in fractional reabsorption of Na<jats:sup>+<\/jats:sup> up to the early distal tubule (FR\u2010Na<jats:sup>+<\/jats:sup>) of 8% and the increase in early distal tubular Na<jats:sup>+<\/jats:sup> concentration ([Na<jats:sup>+<\/jats:sup>]<jats:sub>ED<\/jats:sub>) from 35\u201351\u2003m<jats:sc>M<\/jats:sc> whereas [K<jats:sup>+<\/jats:sup>]<jats:sub>ED<\/jats:sub> was left unaltered.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In comparison, furosemide (3\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup> i.v.), which acts in the water\u2010impermeable thick ascending limb, elicited diuresis, natriuresis and kaliuresis which were associated with a fall in FR\u2010Na<jats:sup>+<\/jats:sup> of 10% with no change in FR\u2010fluid, and a rise in [Na<jats:sup>+<\/jats:sup>]<jats:sub>ED<\/jats:sub> from 42\u2013117\u2003m<jats:sc>M<\/jats:sc> and [K<jats:sup>+<\/jats:sup>]<jats:sub>ED<\/jats:sub> from 1.2\u20135.7\u2003m<jats:sc>M<\/jats:sc> with no change in V<jats:sub>ED<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Direct late proximal tubular fluid collections confirmed a significant inhibition of fluid reabsorption in proximal convoluted tubule in response to <jats:italic>systemic<\/jats:italic> application of U37883A.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings suggest that the diuretic and natriuretic effect upstream to the distal tubule in response to <jats:italic>systemic<\/jats:italic> application of U37883A involves actions on water\u2010permeable segments such as the proximal convoluted tubule.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>127<\/jats:bold>, 1811\u20131818; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702742\">10.1038\/sj.bjp.0702742<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702742","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:24:28Z","timestamp":1106943868000},"page":"1811-1818","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Eukaliuric diuresis and natriuresis in response to the K<sub>ATP<\/sub> channel blocker U37883A: Micropuncture studies on the tubular site of action"],"prefix":"10.1111","volume":"127","author":[{"given":"Dan Y","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hartmut","family":"Osswald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"Vallon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"F496","article-title":"Regulation of basolateral potassium channels in proximal tubule studied during continuous microperfusion","volume":"264","author":"BECK J.S.","year":"1993","journal-title":"Am. 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