{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:54:07Z","timestamp":1761512047982},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":3378,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The role of protein kinase C (PKC) in colonic cellular injury in response to high concentrations of nitric oxide (NO) released from the donor, S\u2010nitroso\u2010N\u2010acetyl\u2010DL\u2010penicillamine (SNAP) was investigated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Addition of SNAP (0.1\u20131000\u2003\u03bc<jats:sc>M<\/jats:sc>) to the cellular suspension resulted in a dose\u2010dependent increase in the extent of damage to isolated colonic mucosal cells as assessed by Trypan blue dye uptake and release of the lysosmal enzyme, N\u2010acetyl\u2010\u03b2\u2010glucosaminidase. SNAP treatment also resulted in an increase in cellular total PKC activity. These increases were reduced or eliminated by pretreatment of the cells with the PKC antagonists staurosporine or GF 109203X or the NO scavenger, phenyl\u20104,4,5,5,\u2010tetramethylimidazoline\u20101\u2010oxyl 3\u2010oxide (PTIO).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>PKC\u2010\u03b1, PKC\u2010\u03b4, PKC\u2010\u03b5 and PKC\u2010\u03b6 were detected in colonic cellular lysates by immunoblotting. However, only PKC\u2010\u03b5 protein was increased in response to SNAP treatment. Furthermore, SNAP treatment resulted in activation of PKC\u2010\u03b5 by causing translocation of the enzyme from the cytosolic to membrane fraction of the cell. This effect was eliminated if cells were preincubated with the NO scavenger, PTIO.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The extent of cellular damage in response to addition of SNAP to the incubation medium was enhanced by coincubation with the PKC activator, phorbol 12\u2010myristate 13\u2010acetate (PMA; 1 and 10\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>PKC activity and the extent of cell damage in response to SNAP were reduced by preincubation of the cells with the peroxyl scavenger, ebselen (0.01\u201310\u2003\u03bc<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data suggest that the PKC\u2010\u03b5 isoform of the enzyme mediates NO\u2010induced damage to colonic mucosal cells. This response may occur, at least in part, due to peroxynitrite formation.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>128<\/jats:bold>, 1268\u20131274; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702891\">10.1038\/sj.bjp.0702891<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702891","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:25:43Z","timestamp":1106943943000},"page":"1268-1274","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The involvement of protein kinase C in nitric oxide\u2010induced damage to rat isolated colonic mucosal cells"],"prefix":"10.1111","volume":"128","author":[{"given":"B L","family":"Tepperman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B D","family":"Soper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.4.1620"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715769509147525"},{"key":"e_1_2_7_4_1","first-page":"A674","article-title":"The effect of protein kinase C activation on colonic epithelial cellular injury","volume":"116","author":"BROWN J.F.","year":"1999","journal-title":"Gastroenterology"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1988.24"},{"key":"e_1_2_7_6_1","first-page":"788","article-title":"Role of c\u2010jun in human myeloid leukemia cell apoptosis induced by pharmacological inhibitors of protein kinase C","volume":"49","author":"FREEMERMAN A.J.","year":"1996","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(96)00024-4"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.1.73"},{"key":"e_1_2_7_9_1","first-page":"1381","article-title":"Localization of protein kinase C isozymes in rat colon","volume":"6","author":"JIANG Y.H.","year":"1997","journal-title":"Cell Growth Diff."},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.272.5.G1109"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1996.1064"},{"key":"e_1_2_7_12_1","first-page":"5627","article-title":"Translocation and enhancement of phosphotransferase activity of protein kinase C following exposure in mouse epidermal cells to oxidants","volume":"49","author":"LARSSON R.","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01392-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(94)00295-X"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490360109"},{"key":"e_1_2_7_16_1","first-page":"371","article-title":"Cellular signalling in thymocyte apoptosis","volume":"4","author":"MCCONKEY D.J.","year":"1992","journal-title":"Semin. Immunol."},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01368-X"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4439(91)90040-G"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb14202.x"},{"key":"e_1_2_7_20_1","first-page":"L551","article-title":"TNF\u03b1 induces peroxynitrite\u2010mediated depletion of lung endothelial glutathione via protein kinase C","volume":"269","author":"PHELPS D.T.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.5.587"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(95)00074-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1996.0127"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365529209000074"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00616-4"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1156"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.274.5.L833"},{"key":"e_1_2_7_28_1","first-page":"24","article-title":"Activation of protein kinase C pathway contributes to hydrogen peroxide\u2010induced increase in epithelial permability","volume":"76","author":"SIFLINGER\u2010BIRNBOIM A.","year":"1992","journal-title":"Lab. Invest."},{"key":"e_1_2_7_29_1","first-page":"929","article-title":"The role of cyclic guanylate monophosphate in nitric oxide\u2010injured injury to rat small intestinal epithelial cells","volume":"284","author":"TEPPERMAN B.L.","year":"1998","journal-title":"J. Pharmacol. Exp. Therap."},{"key":"e_1_2_7_30_1","first-page":"1477","article-title":"Nitric oxide synthase activity, viability and cyclic GMP levels in rat colonic epithelial cells: Effect of endotoxin challenge","volume":"271","author":"TEPPERMAN B.L.","year":"1994","journal-title":"J. Pharmacol. Exp. Therap."},{"key":"e_1_2_7_31_1","first-page":"G214","article-title":"Nitric oxide synthase induction and intestinal epithelial cell viability in the rat","volume":"265","author":"TEPPERMAN B.L.","year":"1993","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_32_1","first-page":"G119","article-title":"Effects of calcium modifying agents on integrity of rabbit isolated gastric mucosal cells","volume":"261","author":"TEPPERMAN B.L.","year":"1991","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)81088-X"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(95)90077-2"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1993.1242"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(98)00105-7"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0702891","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0702891","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0702891","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T00:01:13Z","timestamp":1698537673000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0702891"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,11]]}},"alternative-id":["10.1038\/sj.bjp.0702891"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0702891","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11]]},"assertion":[{"value":"2009-01-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}