{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T14:18:33Z","timestamp":1777645113182,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":3378,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Although the ORL1 receptor is clearly located within the spinal cord, the functional signalling mechanism of the ORL1 receptor in the spinal cord has not been clearly documented. The present study was then to investigate the guanine nucleotide binding protein (G\u2010protein) activation mediated through by the ORL1 receptor in the mouse spinal cord, measuring the modulation of guanosine\u20105\u2032\u2010<jats:italic>o<\/jats:italic>\u2010(3\u2010[<jats:sup>35<\/jats:sup>S]\u2010thio) triphosphate ([<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S) binding by the putative endogenous ligand nociceptin, also referred as orphanin FQ. We also studied the anatomical distribution of nociceptin\u2010like immunoreactivity and nociceptin\u2010stimulated [<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S autoradiography in the spinal cord.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Immunohistochemical staining of mouse spinal cord sections revealed a dense plexus of nociceptin\u2010like immunoreactive fibres in the superficial layers of the dorsal horn throughout the entire length of the spinal cord. In addition, networks of fibres were seen projecting from the lateral border of the dorsal horn to the lateral grey matter and around the central canal.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:italic>In vitro<\/jats:italic> [<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S autoradiography showed high levels of nociceptin\u2010stimulated [<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S binding in the superficial layers of the mouse dorsal horn and around the central canal, corresponding to the areas where nociceptin\u2010like immunoreactive fibres were concentrated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In [<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S membrane assay, nociceptin increased [<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S binding of mouse spinal cord membranes in a concentration\u2010dependent and saturable manner, affording maximal stimulation of 64.1\u00b12.4%. This effect was markedly inhibited by the specific ORL1 receptor antagonist [Phe<jats:sup>1<\/jats:sup>\u03a8 (CH<jats:sub>2<\/jats:sub>\u2010NH) Gly<jats:sup>2<\/jats:sup>] nociceptin (1\u201313) NH<jats:sub>2<\/jats:sub>. None of the \u03bc\u2010, \u03b4\u2010, and \u03ba\u2010opioid and other G\u2010protein\u2010coupled receptor antagonists had a significant effect on basal or nociceptin\u2010stimulated [<jats:sup>35<\/jats:sup>S]\u2010GTP\u03b3S binding.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings suggest that nociceptin\u2010containing fibres terminate in the superficial layers of the dorsal horn and the central canal and that nociceptin released in these areas may selectively stimulate the ORL1 receptor to activate G\u2010protein. Furthermore, the unique pattern of G\u2010protein activation in the present study provide additional evidence that nociceptin is distinct from the \u03bc\u2010, \u03b4\u2010 or \u03ba\u2010opioid system.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1999) <jats:bold>128<\/jats:bold>, 1300\u20131306; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702907\">10.1038\/sj.bjp.0702907<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702907","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T20:25:43Z","timestamp":1106943943000},"page":"1300-1306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Identification of the G\u2010protein\u2010coupled ORL1 receptor in the mouse spinal cord by [<sup>35<\/sup>S]\u2010GTP\u03b3S binding and immunohistochemistry"],"prefix":"10.1111","volume":"128","author":[{"given":"Minoru","family":"Narita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Mizoguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E","family":"Oji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Narita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nae J","family":"Dun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bang H","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Nagase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leon F","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960429)368:2<229::AID-CNE5>3.0.CO;2-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00561-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1977.sp011825"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(76)90042-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1970.33.2.293"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15969.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)80603-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701640"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1985.53.4.1059"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)90925-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(97)13414-0"},{"key":"e_1_2_7_13_1","first-page":"pp","volume-title":"Senspru Functions of the Skin in Prinates, with Special Reference to Man","author":"KUMAZAWA T.","year":"1976"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901620102"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.64010034.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00251-0"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901860202"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901860204"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/377532a0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)80235-1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902200306"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702330"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00395-1"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.76.233"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199802160-00005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(75)90005-6"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5237.792"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199605310-00007"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(97)13453-X"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199611250-00045"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971006)386:4<562::AID-CNE4>3.0.CO;2-0"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.16.7242"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199602290-00012"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(97)00807-0"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15618.x"},{"key":"e_1_2_7_36_1","first-page":"848","article-title":"Modulation by \u03bc\u2010opioid agonists of guanosine\u20105\u2032\u2010O\u2010(3\u2010[35S]thio)\u2010triphosphate binding to membranes from human neuroblastoma SH\u2010SY5Y cells","volume":"47","author":"TRAYNOR J.R.","year":"1995","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90422-0"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00557-5"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90181-3"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00160-8"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1152\/jn.1974.37.2.358","article-title":"Responses of primate spinothalamic tract neurons to natural stimulation of hindlimb","volume":"37","author":"WILLIS W.D.","year":"1974","journal-title":"J. Neurophysiol."},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)00186-8"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0702907","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0702907","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0702907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T00:02:03Z","timestamp":1698537723000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0702907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,11]]}},"alternative-id":["10.1038\/sj.bjp.0702907"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0702907","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11]]},"assertion":[{"value":"2009-01-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}